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博碩士論文 etd-0020115-004447 詳細資訊
Title page for etd-0020115-004447
論文名稱
Title
耐熱新藻種之篩選與鑑定:新穎扁藻的Omega-3脂肪酸及胡蘿蔔素合成初探
Production of long chain omega-3 fatty acids and carotenoids in tropical areas by a new heat-tolerant microalga Tetraselmis sp. DS3
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
43
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2015-01-07
繳交日期
Date of Submission
2015-01-20
關鍵字
Keywords
葉黃素、微藻、耐熱、Omega-3脂肪酸、β-胡蘿蔔素、Tetraselmis
heat-tolerance, microalgae, Tetraselmis sp., Omega-3 fatty acid, lutein, β-carotene
統計
Statistics
本論文已被瀏覽 5842 次,被下載 181
The thesis/dissertation has been browsed 5842 times, has been downloaded 181 times.
中文摘要
暫不公開
Abstract
暫不公開
目次 Table of Contents
致謝 i
摘要 ii
Abstract iii
目錄 iv
表目錄 vi
圖目錄 vii
一、前言 + 1
二、材料和方法 4
2.1微藻樣本、養殖方法及逆境處理條件 4
2.2掃描式電子顯微鏡(Scanning electron microscopy, SEM) 5
2.3 DNA的萃取 5
2.4聚合酶鏈鎖反應(Polymerase Chain Reaction, PCR) 6
2.5定序與序列分析 7
2.6 Tetraselmis sp. DS3於高溫處理下的死亡率測試 7
2.7 Tetraselmis sp. DS3於高溫處理下的葉綠素含量測試 7
2.8 Tetraselmis sp. DS3的雙相變溫養殖 8
2.9總油脂的提取和油脂組成分析 8
2.10高效能液相層析法(High Performance Liquid Chromatography, HPLC) 9
2.11統計分析 11
三、結果與討論 12
3.1耐熱微藻的分離與鑑定 12
3.2 Tetraselmis sp. DS3的耐熱測試 12
3.3 Tetraselmis sp. DS3於逆境下的生長情況 14
3.4 Tetraselmis sp. DS3在不同逆境下生物量、脂質含量及脂肪酸分析 15
3.5 Tetraselmis sp. DS3在不同逆境下的色素分析 16
四、結論 18
五、參考文獻 29
附錄 34
參考文獻 References
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