本研究探討維生素E或氧化狀況何者為影響石斑魚合成蓄積高度不飽和脂肪酸的主因，並使用硒作為除維生素E之外的抗氧化添加物。研究包含兩部分，實驗I飼料以酪蛋白混和魚粉做為蛋白來源，並含有約1% 飼料乾重的高度不飽和脂肪酸，分為添加維生素E和硒 (FSE)、只添加硒 (FS)、只添加維生素E (FE)和兩者均不添加 (F)四處理組。14週飼育結果顯示F組魚體粗脂肪及高度不飽和脂肪酸含量顯著低於FS、FE和FSE組。飼料添加維生素E顯著提高魚體EPA蓄積量，與硒交互作用也顯著。魚體肝臟TBARS濃度顯著受飼料添加維生素E或 (和)硒影響，以FS組最高、FSE組最低，但肝臟TBARS濃度變化與肝臟高度不飽和脂肪酸百分比之間未有相同趨勢。飼料同時添加維生素E和硒時肝臟與肌肉TBARS濃度最低，但飼料只添加維生素E時魚體高度不飽和脂肪酸含量最高，顯示維生素E除抗氧化功能外，可能對石斑魚的高度不飽和脂肪酸代謝還扮演其他角色。實驗II以不含高度不飽和脂肪酸的無維生素酪蛋白做為蛋白來源，分為飼料添加硒 (CS)和不添加 (C)兩處理組，探討抗氧化物對魚體高度不飽和脂肪酸含量的變化，是否由石斑魚合成所致。10週飼育結果顯示飼料添加硒顯著降低肝臟TBARS濃度，但對魚體高度不飽和脂肪酸含量則未有顯著影響。實驗後兩處理組魚體高度不飽和脂肪酸含量均低於初始魚體含量，顯示抗氧化物對石斑魚體高度不飽和脂肪酸含量的影響主要來自於飼料中高度不飽和脂肪酸的吸收或利用率不同所致。綜合兩實驗，維生素E是影響石斑魚高度不飽和脂肪酸含量的主因，當飼料不提供高度不飽和脂肪酸時，抗氧化物對石斑魚合成高度不飽和脂肪酸能力的影響非常有限。

The aim of the study was to investigate which of Vitamin E or oxidative status affect HUFA accumulation and synthesis in juvenile groupers. Two experiments were compared. In the experiment I, juvenile groupers were fed for 14 weeks one of the four HUFA-containing experimental diets: Diet FSE supplemented with both Vitamin E and Se, Diet FE supplemented with Vitamin E, Diet FS supplemented with Se, and Diet F supplemented with neither Vitamin E nor Se. The results of the growth trial showed no significant difference in growth performance. The F group had significantly lower crude lipids and HUFA contents than FS, FE, and FSE groups. Vitamin E supplementation resulted in a significantly increase in EPA accumulation. Its interaction with Se supplementation was significant. TBARS concentration in the liver were significantly affected by dietary Vitamin E and Se supplementation, highest in FS group and lowest in FSE group. There was no similar trend between the percentage of HUFA and concentration of TBARS in the liver. Fish fed Diet FE had the highest level of HUFA, but the FSE group that fed diet with both Vit. E and Se had the lowest TBARS concentration. In the experiment II, juvenile groupers were fed for 10 weeks, two HUFA-free experimental diets with (CS) or without (C) Se supplementation, to investigate which of biosynthesis or dietary residual affect the levels of HUFA by dietary antioxidant supplementation. Se supplementation resulted in a significant decrease in liver concentration of TBARS, but not in whole fish of HUFA levels. HUFA contents in the final fish of both C and CS groups whole body were significant lower than those of the initial fish. These results indicated Vitamin E, but not oxidative status, was the major factor affect if the level of HUFA in the grouper. When the diet was depleted in HUFA, the body HUFA levels in the groupers were derived from dietary residual, not from biosynthesis.

中文摘要…………………………………………………………………………………i
英文摘要…………………………………………………………………...……………ii
目錄………………………………………………………………………………..……iv
圖目錄…………………………………………………………………………………...v
表目錄………………………………………………………………………………......vi
文獻回顧………………………………………………………………………………...1
前言……………………………………………………………………….……………10
材料與方法………………………………………………………………….…………12
結果…………………………………………………………………………….………31
討論………………………………………………………………………………….…57
結論………………………………………………………………………………….…63
參考文獻………………………………………………………………………….……64

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