根據世界衛生組織(WHO)的最新統計資料顯示，肝癌在全球常見的癌症中位居第六，而主要發生區域多位於亞洲及非洲。且根據衛生福利部統計的結果指出，肝癌是台灣癌症死因的第二位。肝癌目前的治療方式，主要有肝臟移植、手術切除、標靶治療等方式。由於這些肝癌治療的效果還是有限的，因此最有效的方式還是預防，而肝癌患者也需要其他能夠降低治療後癌症復發的藥物。先前已有文獻指出藻類富含許多對人體有益的物質，其中包含許多蛋白質、礦物質與類胡蘿蔔素及必需脂肪酸；而這些物質已有文獻指出具有抗癌及預防癌症等等的功效。因此我們利用先前已篩選出的一株淡水微藻進行本研究，在細胞實驗上，我們的研究發現該微藻萃取物能夠抑制腫瘤進程，在肝癌細胞中主要的機轉是經由阻斷第二型環氧化酶(COX-2)而抑制腫瘤微環境中的前列腺素(PGE2)以及肝癌衍生生長因子(HDGF)而影響肝癌細胞的功能。並於動物實驗的結果得知微藻萃取物在大鼠原位肝癌模式中具有預防的效果並且可以抑制肝腫瘤的生長。

According to WHO statistics shows that hepatocellular carcinoma (HCC) is the sixth most common cancer in the world, and the highest incidence of liver cancer was in Asia and Africa. In addition to the statistics from MOHW indicates that HCC is the second death cause of cancer. Current HCC therapies has liver transplant, surgical resection and target therapy, etc. However, these therapies of HCC still has limited, thus the most effective way is prevention, and the patient of liver cancer need more other drugs which reduce the risk of relapse. Previous studies has been indicated that microalgae are benefit for health which contains proteins, minerals, carotenoid and essential fatty acids. These materials also can prevent cancer and the effect of anti-cancer, thus we use previously screened freshwater microalga conduct this study. We found that green alga extract inhibits tumor progression and the mechanism is that green alga extract inhibited functions of hepatoma cells through reduced tumor micro-environmental prostaglandin E2 (PGE2) and hepatoma-derived growth factor (HDGF) in vitro. We also obtains that green alga extract has prevention efficacy and inhibition of tumor growth in rat orthotopic hepatoma model.

Verification letter from the Oral Examination Committee i
Acknowledgement ii
摘要 iii
Abstract iv
List of Tables and Figures vi
Introduction 1
Materials and methods 6
Results 15
Discussion 25
Figures and legends 31
Conclusion 64
Future perspectives 65
References 66
Appendix 72

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