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博碩士論文 etd-0904112-181200 詳細資訊
Title page for etd-0904112-181200
論文名稱 Title |
直接式電噴灑探針質譜法應用在植物系統中化學成份改變的研究 Applications of Direct Electrospray Probe Mass Spectrometry in Studying Change of Chemical Compounds in Plants |
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系所名稱 Department |
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畢業學年期 Year, semester |
語文別 Language |
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學位類別 Degree |
頁數 Number of pages |
84 |
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研究生 Author |
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指導教授 Advisor |
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召集委員 Convenor |
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口試委員 Advisory Committee |
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口試日期 Date of Exam |
2012-08-23 |
繳交日期 Date of Submission |
2012-09-04 |
關鍵字 Keywords |
大氣壓力質譜法、直接式電噴灑探針、取樣探針、植物農藥吸收、辣椒素分佈、馬鈴薯表皮毒素 potato surface, capsaicin distribution, pesticide absorption, sampling probe, direct electrospray probe, ambient mass spectrometry |
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統計 Statistics |
本論文已被瀏覽 5697 次，被下載 1656 次 The thesis/dissertation has been browsed 5697 times, has been downloaded 1656 times. |
中文摘要 |
大氣游離源 ( Ambient Ionization Source ) 為現今質譜技術發展的主流之一，其優點為快速與簡單操作，因此近年有許多相關技術的開發與發表，甚至許多商業化的產品推出，而直接式電噴灑探針 ( Direct Electrospray Probe )技術亦是大氣游離源的一支，強調無前處理步驟的快速細微結構直接採樣分析。 本研究改良了直接式電噴灑探針使其更適合真實樣品的快速分析，省略了原本提供電噴灑液所需要的管線與注射針幫浦，改為在直接式電噴灑探針上增加電噴灑溶液儲存槽，以重力方式使電噴灑溶液自然流出，減少取樣時管線拆裝的限制，電壓施加方式改由以質譜儀提供，探針部份以電刷片接地增加操作時安全性，最後最佳化其位置增加穩定性。 本研究中將其技術應用在植物的分析上，分析不同成熟度的辣椒果實，成功的在不同成熟度的辣椒中測得辣椒素的訊號，推測辣椒素生成部位；也應用在分析蕃茄植株吸收農藥納乃得之過程，成功在各部位得到納乃得訊號；最後分析馬鈴薯表面毒素要出芽處的分佈，驗證了直接式電噴灑探針在非平面表面採樣與空間分析上的能力。 |
Abstract |
Ambient mass spectrometry is one of the mainstream techniques in modem mass spectrometry development. The developing purpose of ambient mass spectrometry is improving the analysis efficiency and simplifying operation process. So that, there are many techniques about ambient mass spectrometry had been published and commercialized. And direct electrospray probe mass spectrometry (DEP/MS) is one of ambient mass spectrometry techniques which is emphasized its rapid analysis and high sampling space resolution. In this research we improved the DEP system for more suitable in rapid analysis. We rule of the use of Syringe pump and its tube. Replace it by using solution tank so that we can save the time cost by disassemble syringe pump and tube when sampling. We also change the electric field by different high voltage applied mode for safety concern. In this study DEP/MS were used in plants analysis applications: capsaicin in peppers, systematic pesticide absorption in tomato and toxic compounds distribution in potato surface. |
目次 Table of Contents |
目錄 論文審定書 ......................................................... I 謝誌 .............................................................. II 中文摘要 .......................................................... IV 英文摘要 .......................................................... V 目錄 .............................................................. VI 圖目錄 ...................................................................................................................... VII 表目錄 ........................................................................................................................ XI 壹、緒論 ........................................................................................................................... 1 一、前言 ................................................................................................................. 1 二、傳統真空游離技術 ............................................................................................ 5 三、現代有機質譜游離源 ( Modern Organic Mass Ionization Source ) ................ 7 四、大氣質譜游離源 ( Ambient Mass Ionization Source ) .................................. 13 五、論文目標 .......................................................................................................... 24 貳、實驗 .......................................................................................................................... 25 一、實驗藥品與試劑 .............................................................................................. 25 二、儀器裝置 .......................................................................................................... 26 三、藥品配置 ........................................................................................................ 28 四、分析操作流程 .................................................................................................. 28 參、結果與討論 ........................................................................................................... 29 一、直接式電噴灑探針的改良 .............................................................................. 29 二、以直接式電噴灑探針偵測辣椒中之辣椒素 .................................................. 42 三、以直接式電噴灑探針偵測植物吸收現象 ...................................................... 51 四、以直接式電噴灑探針偵測馬鈴薯表面毒素 .................................................. 65 肆、結論 .......................................................................................................................... 68 伍、參考資料 ................................................................................................................ 70 |
參考文獻 References |
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