戴奧辛降解菌--門多薩假單胞菌菌株，已自戴奧辛污染土壤中，經篩選純化得到。本研究探討菌株對多氯戴奧辛/呋喃、多溴戴奧辛/呋喃、多溴二苯醚、多氯聯苯及多環芳香族碳氫化合物之生物降解能力。本研究以高解析度氣相層析質譜儀(HRGC/HRMS) 及化學活性冷光表現法 (CALUX)，進行類戴奧辛化合物之含量分析。結果顯示，菌株能在厭氧狀態下液態培養液中，降解多氯戴奧辛/呋喃，並已確認主要的中間代謝產物。生物反應器結果顯示，菌株能在好氧狀態下泥漿相中，有效降解多氯戴奧辛/呋喃，實驗結果顯示，60天後八氯戴奧辛及呋喃，降解率分別為97% 及98%。泥漿相生物反應器中，戴奧辛濃度之總毒性當量由5,823降至1,198 pg-TEQ/g，其降解去除率為79% 。菌株在好氧狀態下液態培養液中，亦能對多溴戴奧辛/呋喃、多溴二苯醚、多氯聯苯及多環芳香族碳氫化合物進行生物降解。透過本研究確認菌株具備生物整治技術之潛力，未來能應用於類戴奧辛化合物污染土壤之整治。

The dioxin-degrading bacterium Pseudomonas mendocina strain NSYSU (NSYSU strain) has been isolated from dioxin-contaminated soil by selective enrichment techniques. In this study, the NSYSU strain was investigated for its capability to biodegrade polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs), polybrominated dibenzo-p-dioxins/dibenzofurans (PBDD/Fs), polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs). High-resolution gas chromatography-mass spectrometry (HRGC/HRMS) and a Chemically Activated Luciferase Gene Expression (CALUX) by Xenobiotic Detection Systems (XDS) bioassay were performed to determine the concentration of dioxin-like compounds. The results indicate that the NSYSU strain could degrade PCDDs and PCDFs under anaerobic conditions in liquid cultures. The main intermediates of the dechlorination process were identified. The results of the bioreactor test indicate that the NSYSU strain could also degrade PCDDs and PCDFs effectively in soil slurries under aerobic conditions. Results from the bioreactor experiment show that approximately 98 and 97% of OCDF and OCDD were degraded in 60 days, respectively. The dioxin concentrations in soil slurry decreased from 5,823 to 1,198 pg-TEQ/g, and that resulted in a 79% of total dioxin removal. The NSYSU strain also could biodegrade PBDD/Fs, PBDEs, PCBs and PAHs effectively under aerobic conditions in liquid cultures. These first findings suggest that the strain has the potential to be an effective tool for the bioremediation of soils contaminated with dioxin-like compounds.

Index
Certificate …..………………………………………………………… i
Acknowledgements …………………………………………………… ii
Abstract (Chinese) ..……………………………………………….….. iii
Abstract ..….……………………………………..……………………. iv
Chapter 1 Introduction …………………………………………………… 1
Chapter 2 Material and Method .……………………………….………… 9
2.1 Chemicals ………………………………………………………… 9
2.2. Incubation conditions for P. mendocina strain NSYSU..…………9
2.3. Anaerobic degradation of PCDDs and PCDFs by P. mendocina
strain NSYSU ……………………………………………………10
2.4. Collection of dioxin-contaminated soil .……….………………… 11
2.5. Bioreactor test………………………… .…………………………11
2.6. Aerobic degradation of dioxin-like compounds by P. mendocina
NSYSU .……………………………………..……………………12
2.7. Chemically activated luciferase gene expression (CALUX)
bioassay for soil samples.…………………………………………13
2.8. HRGC/HRMS analysis………………………….…………………14
2.9. PCR analysis and microbial/gene identification ..…………………15
Chapter 3. Results and Discussion .………………………………………16
3.1. The growth of P. mendocina strain NSYSU……………………….16
3.2. Anaerobic degradation of PCDDs and PCDFs by P. mendocina
strain NSYSU……………………………………………………...16
3.3 Bioreactor test ….…………………………….…………………….. 18
3.4. CALUX bioassay ………………………………………………….. 21
3.5. Aerobic degradation of dioxin-like compounds ..………………….. 21
3.6. PCR analysis and microbial/gene identification …………………... 22
Chapter 4. Conclusions…………………………………………….……..… 24
References .………………………………………………………….………26
Figure Captions ……………………………………………………………..33
Table Captions ………………………………………………………………44
Appendix ……………………………………………………………………51
Patent 1.
Microbial reagents for scavenging dioxin pollutants present in a contaminated medium and methods of using the same ……………..………..………...52
Patent 2.
Inducement method for enhancing degradation efficiency of dioxin-degrading dacteria …………………..…………………………..…………………...116

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