本研究係使用戶外養殖系統，進行藻株優異性比較，結果證實Scenedesmus obliquus CNW-N在相同外在培養環境下，比其他兩藻種成長速率更佳。故以Scenedesmus obliquus CNW-N作為廢水養殖實驗之藻株。
廢水中含有許多汙染物，換個角度來看，這些汙染物是植物生長所必須之營養鹽。為進行廢水再利用最佳配方之比較研究，本研究分兩階段實驗。首先利用廢水添加市售農用肥料(添加量：1g/L)與DM配方做比較，於兩種不同營養鹽作為培養基下，DM配方之生長速率平均為117.5(mg/L/day)，優於中水添加市售農用肥料，其生長速率為95.0(mg/L/day)，證實DM配方優於市售肥料。隨後為提升添加市售農用肥料之生長速率，分析市售農用肥料之營養鹽含量，並與DM配方作等量比較，當肥料添加量為0.5083g/L時，其氮量相等，其餘磷、鉀、鎂等營養鹽需額外補充。並以此為根據進行第二次實驗，由實驗數據證實，DM配方之生長效率平均為88.33(mg/L/day)，中水+肥料+磷鉀鎂等營養鹽生長效率為89.17(mg/L/day)，證實後者與前者生長速率相當，但成本低廉。故建議未來可以使用中水+肥料+磷鉀鎂等營養鹽配方方式進行大規模之養殖。

In this study, the use of outdoor cultivation system for algae strains performance comparison, the results confirm that at the same environmental conditions, the growth rate of Scenedesmus obliquus CNW-N train is better than the other two species. Thus, the Scenedesmus obliquus CNW-N was chosen as the only candidate for testing the wastewater aquaculture experiments.
From agricultural point of view, wastewater contains a verity of nutrient which is necessary for plants growing. In order to identify the best formula added in wastewater, this study includes two phase experiments. Firstly, the growth rate testing of the wastewater adding commercial agricultural fertilizer (adding amount 1g/L) comparing with DM formula. The results indicated that the growth rate of the DM was 117.5 mg/L/day better than wastewater adding commercially available agricultural fertilizer whose growth rate was 95.0 mg/L/day. To improve the growth rate of the case of wastewater adding fertilizer, a nutrient content analysis of commercial agricultural fertilizer has been carried out to compare the same amount of DM recipe. It found that when the fertilizer dosage 0.5083g/L, it will generate the same amount of nitrogen, but phosphorus, potassium, and magnesium are insufficient and must be supplied through other supplement source. Based on this equal nutrient condition, the second experiment was conducted and found that the average growth rate of the set of DM formula was 88.33 mg/L/day, while the set of wastewater + fertilizer + extra-nutrient-supplement was 89.17 mg/L/day. This results confirmed that the growth rate of the latter is slightly better that the former, but the price is considerably cheaper, and is recommended for the large-scale farming system.

目錄
摘要 i
Abstract ii
目錄 iii
圖次 vii
表次 x
第一章 緒論 1
1.1前言 1
1.2研究目的 2
第二章 文獻回顧 4
2.1藻類演化與分類 4
2.2藻類生長 7
2.2.1二氧化碳對藻類之影響 7
2.2.2 pH值對藻類之影響 8
2.2.3光對藻類之影響 9
2.2.4營養鹽對藻類之影響 10
2.2.5溫度對藻類之影響 14
2.3微藻培養系統 14
2.4微生物的生長週期 17
2.5放流水標準 19
第三章 實驗材料及方法 20
3.1中山大學汙水處理廠簡介 20
3.2實驗器材與設備 21
3.2.1光反應器 21
3.2.2進氣系統 22
3.2.3二氧化碳鋼瓶 23
3.2.4流量計 24
3.2.5 LI-COR光度計 24
3.2.6烘箱 24
3.2.7滅菌釜 25
3.3 藻種來源與養殖放大流程 26
3.3.1室內養殖 27
3.3.2室外養殖 27
3.4實驗分析方法 28
3.4.1分光光度計之吸光度 29
3.4.2微藻濃度分析 30
3.4.3 OD與Biomass關係曲線 33
3.5實驗步驟 34
第四章 實驗結果與討論 37
4.1藻株成長優異性比較 37
4.2營養鹽配方比較實驗(ㄧ) 40
4.2.1實驗條件 40
4.2.2市售農用肥料與DM配方營養鹽比較 41
4.2.3實驗過程營養鹽之變化量 41
4.2.4實驗期間光照及氣溫變化 43
4.2.5不同條件下微藻成長狀態分析 45
4.3營養鹽配方比較實驗(二) 46
4.3.1實驗條件 46
4.3.2市售農用肥料與DM配方主要營養鹽差異之比較 47
4.3.3實驗過程營養鹽之變化量 47
4.3.4實驗期間光照及氣溫變化 48
4.3.5各實驗組微藻成長結果 50
4.4 PH值與微藻生長關係 51
4.5 中水營養鹽成分對產率之影響 53
4.6 實驗(二)營養鹽配方成本比較 54
第五章 結論與建議 56
5.1 結論 56
5.2 建議 57
參考文獻 58
附錄A放流水標準 64
附錄B 營養鹽配方計算及成本估算 81

參考文獻
1. 黃春蘭. 2003. 水質學. 藝軒圖書出版社.
2. 郝道猛. 1992. 生態學概論. 科學圖書大庫. 徐氏基金會出版. 台北市.
3. 鄭恆琪. 1995. 環境因子控制chlorella minutissima之化學及脂肪酸. 農業化學研究所, 碩士, 國立台灣大學. 台北市.
4. 張乃尤. 2011. 藻類生長的影響因子-以豬場廢水培養液為例. 環境工程與科學研究所, 碩士, 國立屏東科技大學. 屏東縣內埔鄉.
5. 蔡安益. 1997. 台灣西南沿海水域冬季枯水期營養鹽限制因素之研究-營養鹽添加實驗. 漁業科學學系, 碩士, 國立臺灣海洋大學. 基隆市.
6. 黃巧旻. 2011. 探討固定化微藻攝取營養鹽與生產油脂之研究. 水資源及環境工程學系, 碩士, 淡江大學. 新北市.
7. 謝誌鴻. 2009. 微藻培養與微藻油脂生產之研究. 化學工程學系, 博士, 國立成功大學. 台南市.
8. 周延耀. 2003. 碳源的添加對等邊金藻增值的影響. 海洋生物研究所, 碩士, 國立中山大學. 高雄市.
9. 李雪甄. 2009. 降低pH值對鹼性土壤磷有效性及型態的影響. 農藝學系, 碩士, 國立嘉義大學. 嘉義市.
10. 賀詩欣. 2012. 建立本土微藻Scenedesmus obliquus CNW-N之最適化微藻二氧化碳固定與生質酒精生產程序. 化學工程學系, 博士, 國立成功大學. 台南市.
11. 陳鍠文. 2013. 戶外直立式光生物反應器日照衰減之研究. 海洋環境及工程學系, 碩士, 國立中山大學. 高雄市
12. 范萬釗, 李錫霖. 2003. 從中水回收再利用探討節水技術與污水資源化 水資源之永續發展. 土木工程研討會, pp.c19~c28.
13. 潘崇良. 2012. BioEnergyToday 生質能源趨勢. 國立台灣海洋大學食品科學系. 基隆市.
14. 教育部數位教學資源入口網，教科書，第三章 養分與能量https://isp.moe.edu.tw/
15. 台灣海洋生態資訊學網. http://study.nmmba.gov.tw/01_room/encyclopedia.aspx?enc_rid=125&res_objno=ossary0125
16. Andersen, T. F., and Andersen, Q., 2006, “Effects of CO2 concentration on growth of filamentous algae and Littorella uniflora in a Danish softwater lake,”Aquatic Botany, Vol. 84, pp.267-271.
17. Apt,KE;Behrens,PW. 1999.Commercial developments in microalgal biotechnology.J.Phycol.35(2):215-226.
18. Becker,E.W.,1994,Microalgae ：biotechnology and microbiology,University of Cambridge Press,New York,pp.293
19. Chen, F. 1996. High cell density culture of microalgae in heterotrophic growth. Trends Biotechnol. 14(11):421-426.
20. Fernandez, FGA; Sevilla, JMF; Perez, JAS; Grima, EM; Chisti, Y.2001. Airlift-driven external-loop tubular photobioreactors for outdoor production of microalgae: assessment of design and performance. Chem. Eng.Sci. 56(8):2721-2732.
21. Fredeen, A. L., Rao, I. M., and Terry, N., 1989, “Influence of phosphate nutrition on growth and carbon partitioning in Glycine max,”Plant Physiology,Vol.89,pp.225-230.
22. Ho, S.H.,Chen, C.Y.,Yeh, K.L.,Chen, W.M.,Lin, C.Y.,and Chang, J.S., 2010, “Characterization of photosynthetic carbon dioxide fixation ability of Indigenous Scenedesmus obliquus isolates,”Biochemical Engineering Journal, Vol. 53, pp.57-62.
23. Hu, Q; Guterman, H; Richmond, A. A flat inclined modular photobioreactor for outdoor mass cultivation of photoautrophs. Biotechnol. Bioeng. 51(1): 51-60.1996a
24. Kaplan, D., Cohen, Z., and Abeliovich, A., 1986, “Optimal growth conditions for Isochrysis galbana .Biomass,”Vol.9,pp.37-48.
25. Kozlowska-Szerenos, B., Zielin’ski, P., and Maleszewski, S., 2000,“Involvement of glycolate metabolism in acclimation of Chlorella vulgaris cultures to low phosphate supply,”Plant Physiology Biochemistry, Vol.38pp.727-734
26. Laliberte, G; Delanoue, J. 1993. Autotrophic, heterotrophic, and mixotrophic growth of chlamydomonas-humicola(chlorophyceae) on acetate. J. Phycol. 29(5):612-620.
27. Morita, M; Watanabe, Y; Saiki, H. 2000. Investigation of photobioreactor design for enhancing the photosynthetic productivity of microalgae. Biotechnol. Bioeng. 69(6):693-698.
28. Pulz, O. 2001.Photobioreactors: production systems for phototrophic microorganisms. Applied Microbiology and Biotechnology, 57:287-293.
29. Maio, X., and Wu, Q., 2006, “Biodiesel production from heterotrophic microalgal oil,”Bioresource Technology Vol. 97 ,pp. 841-846.
30. Mimura, T., 1995,“Homeostasis and transport of inorganic phosphate in plants,”Plant Cell Physiology, Vol.36,pp.1-7.
31. Morris, I. 1980, The Physiological Ecology of Phytoplankton, Blackwell Scientific Publication, Oxford, pp. 625.
32. Raven,P.H.,Johnson,G.B., 2002, “Biology”,McGraw-Hill,sixth edition.
33. Rubio, FC; Fernandez, FGA; Perez, JAS; Camacho, FG; Grima, FM. 1999.Prediction of dissolved oxygen and carbon dioxide concentration profiles in tubular photobioreactors for microalgal culture. Biotechnol. Bioeng. 62(1): 71-86.
34. Sah, J. G., and Peng, Y. H.,2009, “Study on the growth conditions of fresh water microalgae for CO2 bio-fixation,”The 4th Academic Seminar of National Pingtung University of Sceince and Technology and Beijing University
35. Sato, T; Usui, S; Tsuchiya, Y; Kondo, Y. 2006.Invention of outdoor closed type photobioreactor for microalgae. Energy Conv.Manag.47(6):791-799.
36. Terry,K.L.& Raymond,L.p.1985.System ─ design for the autotrophic production of microalgae.Enzyme and Microbial Technology,7：474-487
37. Theodorou, M. E.,Elrifi, I. R., Turpin, D. H., and Plaxton, W. C., 1991,“Effects of phosphorus limitation on respiratory metabolism in the green alga Selenastrum minutum,”Plant Physiology, Vol.95,pp.1089-1095.
38. Travieso,L.,Beni’tez,F.,Sa’nchez,E.,Borja,R.,Mart’inc,A.,and Colmenarejo,M.F.,2006,“Batch mixed culture of Chlorella vulgaris using settled and diluted piggery waste ,”Ecological Engineering,Vol.28,pp.158-165.
39. Turner, M. A.,Howell, E. T., Robinson, G.G. C., Brewster, J. F., Sigurdson, L. J., and Findlay, D. L., 1995, “Growth characteristics of bloom-forming filamentous green algae in the littoral zone of an experimentally acidified lake,”Canadian Journal of Fisheries and Aquatic Science, Vol. 52, pp.2251-2263.
40. Wen, ZY; Chen, F. 2003.Heterotrophic production of eicosapentaenoic acid by microalgae. Biotechnol.Adv.21(4):273-294.
41. Yang, Y. F., Feng, C. P., Inamori Y., and Maekawa, T., 2004, “Analysis of energy conversion characteristics in liquefaction of algae,”Resources, Conservation and Recycling, Vol. 43,pp.21-33.
42. Yoon, J. H., Shin, J. H., and Pank, T. H., 2008, “Characterization of factors influencing the growth of Anabaena variabilis in a bubble column,”Bioresource technology,Vol.99,pp.1204-1210.
43. Zittelli, GC; Pastorelli, R; Tredici,MR. 2000.A Modular Flat Panel Photobioreactor(MFPP) for indoor mass cultivation of Nannochloropsis sp.under artificial illumination. J. Appl. Phycol. 12(3-5):521-526.