本研究以本土分離純化而得之斜生柵藻Scenedesmus obliquus CNW-N，在戶外以柱狀光生化反應器（Column Photobioreactor）進行栽培。由於批次養殖無法滿足大規模生產的要求，因此本研究嘗試以半連續式系統，並搭配不同來源的水、不同種類的營養鹽及不同型式的養殖法進行實驗。
野外培養的過程，微藻被其他微生物感染是導致養殖過程失敗的主要因子。而氯化消毒為操作成本低又可確實消毒的方法，因此本研究利用氯化消毒法為基礎，建構而成一套可完整操作的半連續式微藻養殖方式。結果顯示在四天的回收週期下，利用自來水搭配肥料培養可得最佳的生物質產率，其每日單位體積下平均產率為58.75 mg dry weight/L。
研究中亦顯示生活汙水處理廠的放流水亦可做為養殖水源，在消毒並適度添加營養後其產率與生長速度相近於以自來水搭配肥料養殖。但放流水中生物無法完全去除，因此到養殖後期易因其他微生物孳生干擾而難以繼續進行培養。結論，利用半連續式系統以兩種不同的培養方式進行戶外大量養殖，研究結果顯示皆可穩定生產微藻。

Scenedesmus obliquus CNW-N, a native algal species obtained from southern Taiwan(Ho et al., 2010) is utilized to cultivate microalgae by using column photobioreactors. Previous studies have indicated that batch culture is not capable to satisfy the requirements of large-scale production of algae. Therefore, this study attempts to develop a semi-continuous culture system to produce large quantity of microalgae using different sources of water, kinds of nutrient and types of methods.
The microbial contamination is one of the major factors leading to the failure of microalgae cultivation. Chlorination is a cost effective and reliable method in disinfection the medium of microalgae. Thus it has been established a feasible semi-continuous cultivation procedure by using Chlorination as the foundation in the study. The experimental results reveal that using tap water with fertilizer could achieve the best biomass yield: average daily yield is 58.75 mg dry weight/L in the four-day recovery period.
This article also shows that effluent coming from sewage treatment plant can be used as breeding water whose yield and growth rate is comparable to the tap water with fertilizer farming after disinfection and adding nutrients. But microorganism existing in the effluent cannot be totally removed, so that S. obliquus CNW-N is hardly to re-cultivated. In conclusion, the reliability has been achieved by utilizing a semi-continuous culture system in two types of methods to cultivate mass microalgae at outdoor condition.

摘要 i
ABSTRACT ii
目錄 iii
圖目錄 viii
表目錄 xi
第一章 緒論 1
1.1 研究動機 1
1.2 研究目的 3
1.3 研究架構 5
第二章 文獻回顧 6
2.1 影響微藻生長之化學因子 6
2.1.1 二氧化碳 6
2.1.2 氮源 6
2.1.3 磷 7
2.2 光合作用 8
2.3 微藻培養方法 9
2.3.1 批次培養 9
2.3.2 連續式培養 9
2.3.3 半連續式培養 10
2.4 水質消毒 11
2.4.1 消毒方法 11
2.4.1.1 化學消毒 11
2.4.1.2 紫外線消毒 12
2.4.2 氯化消毒法 12
2.5 汙水特性 13
2.5.1 汙水定義 13
2.5.1.1 公共汙水之無機物 13
1. 鹽類 13
2. 鹼度 14
3. 氮、磷 14
4. 硫 14
2.5.1.2 公共汙水之有機物 15
1. 碳水化合物 15
2. 蛋白質及氮化合物 15
3. 脂質 16
第三章 材料與方法 17
3.1 藻種 17
3.2 戶外養殖系統建立 19
3.2.1 養殖設備介紹 20
3.2.1.1 光生化反應槽；培養微藻 20
3.2.1.2 回收槽: 儲水與回收 21
3.2.1.3 供氣系統：提供攪拌動力與碳源 21
3.3 養殖操作流程及步驟 23
3.3.1 操作流程 23
3.3.2 操作步驟 24
1. 器材清潔與培養水消毒 24
2. 接種 25
3. 營養鹽 26
4. 碳源 27
3.3.2.1 微藻回收 27
3.4 環境數據測定與生長量測 28
3.4.1 光度計率定 28
3.4.1 濁度計率定 31
3.4.2 溫度監測 34
3.4.3 水質監測 34
3.5 實驗分析方法 35
3.5.1 微藻濃度分析方法 35
3.5.2 生物質量估算 37
3.5.3 比生長速率計算 37
第四章 結果與討論 38
4.1 批次養殖實驗 38
4.1.1 目的及養殖條件 38
4.1.2 成果討論 38
4.2 半連續式養殖實驗 43
4.2.1 養殖之目的及實驗條件 43
4.2.2 養殖期間之環境因子變化 44
4.2.3 四日回收週期之養殖結果 45
4.2.4 六日回收週期之養殖結果 50
4.2.5 pH值監控結果 54
4.2.6 生物質變化監測結果 56
第五章 結論與建議 57
5.1 結論 57
5.2 建議 58
參考文獻 60
附錄A 國立中山大學汙水處理廠簡介 65
A.1. 處理設備簡介 65
A.2. 汙水處理廠前處理 65
A.3. 汙水處理廠後處理 66
A.4. 放流水水質分析 67
附錄B 氯化消毒劑之計量與還原 68
附錄C 半連續式養殖之無機性營養鹽消耗 70
C.1 四天回收組 70
C1.1 200L光反應器 70
C1.2 300L光反應器 72
C.2 六天回收組 74
C2.1 200L光反應器 74
C2.2 300L光反應器 75
附錄D 實驗設備 78

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