Title page for etd-0119110-124444


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URN etd-0119110-124444
Author Guan-Hsien Lee
Author's Email Address No Public.
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Department Institute of Biomedical Sciences
Year 2009
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Molecular epidemiology of norovirus gastroenteritis in children
Date of Defense 2009-09-22
Page Count 53
Keyword
  • norovirus
  • reverse transcription
  • gastroenteritis
  • Abstract The noroviruses are important pathogen of epidemic and sporadic gastroenteritis in all age group and show great genetic diversity.
    The aim of the present study was to describe the prevalence and genetic diversity of noroviruses among children hospitalized with acute sporadic gastroenteritis in Kaohsiung, Taiwan.
    Fecal samples were collected from hospitalized pediatric patients with sporadic gastroenteritis below age of 18 years during a 2-year period (2007 to 2008). Norovirus RNA was detected by reverse transcription-polymerase chain reaction and comfirmed by sequence analysis. Two different sets of primers were used. Region C primers target shell domain at 5’ end of capsid gene and region D primers target highly variable P2 subdomain at 3’ end of capsid gene.
    Noroviruses were identified in 5 of 39 (12%) rotavirus-negative specimens using region C primers. Using region D primers only one among these 5 samples could yield PCR product, which showed concordant noroviral genotype. 3 (10%, n=30) specimens from children below age of 5 years tested positive. All these 5 patients had symptoms of vomiting and 3 had fever. All PCR products were sequenced and showed 2 strains of genogroup 1 (G 1.4) and 3 strains of genogroup2 (G 2.4).
    To our knowledge, this is the first report that demostrated G1.4 genotype norovirus from Taiwan. Norovirus accounted for 10% of sporadic non-bacterial gastroenteritis cases among hospitalized children below 5 years of age in Kaohsiung, Taiwan.
    Advisory Committee
  • Long-Sen Chang - chair
  • Yung-Feng Huang - co-chair
  • Hurng-Wern Huang - advisor
  • Files
  • etd-0119110-124444.pdf
  • indicate in-campus access in a year and off_campus not accessible
    Date of Submission 2010-01-19

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