本篇論文利用磁性氧化鐵奈米粒子 (Iron Oxide Nanoparticles，Fe3O4 NPs) 的化學性質，配合修飾上聚苯乙烯磺酸 (Polystyrene sulfonic acid，PSS) 塗層包覆(PSS-Fe3O4 NPs)，做為吸附劑和非均相 Fenton reaction 催化劑以降解有機污染物。而接續的第二部分研究，利用 Fe3O4 NPs 結合三磷酸腺苷螢光試劑 (ATP-FL) 配合螢光儀檢測三磷酸腺苷 (ATP) 的濃度。
一、利用修飾聚苯乙烯磺酸塗層的四氧化三鐵奈米粒子降解環境水樣中的有機染料亞甲基藍與羅丹明B：
PSS-Fe3O4 NPs 擁有類似辣根過氧化物酶 （Horseradish peroxidase，HRP）的催化能力，可以在過氧化氫(H2O2)的存在下，催化與過氧化氫相關氧化反應中的過氧化物酶受質，進而產生比色或螢光信號。本實驗中用 PSS-Fe3O4 奈米粒子降解常見有機染料，亞甲基藍 (Methylene blue) 和羅丹明 B(Rhodamine B)。降解的過程以紫外光/可見光譜儀分析，染料吸收光譜的強度會隨著反應時間增加而下降。首先 PSS-Fe3O4 奈米粒子與溶液中有機染料進行吸附過程，接著在添加過氧化氫溶液後開始降解反應，反應兩個小時後可移除溶液中97.8 % 的亞甲基藍 (10 mL，50 μM) 或七個小時後移除96.6 % 的羅丹明B (10 mL，5 μM)。實驗結果顯示，1克的 PSS-Fe3O4 奈米粒子可降解 1.8 毫克的亞甲基藍或 2.7 毫克的羅丹明B。
二、利用磁性氧化鐵奈米粒子結合三磷酸腺苷螢光試劑檢測三磷酸腺苷：
在本篇研究中，利用 Fe3O4 奈米粒子表面的 Fe3+ 與 Fe2+ 對三磷酸腺苷螢光試劑 (BODIPY FL ATP，簡稱ATP-FL) 有螢光消光的現象，當加入三磷酸腺苷 (Adenosine triphosphate，ATP) 時會抑制消光的反應，使螢光訊號升高。因此隨著添加的 ATP 反應濃度越高螢光訊號也會隨之增高，我們藉由此方法來偵測 ATP ，最後可應用在血球真實樣品中 ATP 的濃度檢測。

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論文審定書 ....................................................................................................................... i
摘要 .................................................................................................................................. ii
目錄 ................................................................................................................................. iii
圖目錄 ............................................................................................................................. vi
縮寫表 ............................................................................................................................. ix
第一章利用修飾聚苯乙烯磺酸塗層的四氧化三鐵奈米粒子降解環境水樣中的有
機染料亞甲基藍與羅丹明B .......................................................................................... 1
一、前言 .......................................................................................................................... 1
1.1 磁性奈米粒子 ........................................................................................................... 1
1.2 染料 (Dye) ................................................................................................................ 2
1.3 染料污水的處理方法 ............................................................................................... 2
1.4 高級氧化處理程序 .................................................................................................... 2
1.5 Fenton 氧化法程序 .................................................................................................... 3
二、實驗部分 .................................................................................................................. 6
2.1 藥品 ........................................................................................................................... 6
2.2 儀器裝置 .................................................................................................................. 7
2.3 磁性氧化鐵奈米粒子之合成與修飾 ...................................................................... 11
2.3.1 合成Bare Fe3O4 奈米粒子 ........................................................................... 11
2.3.2 合成PSS-Fe3O4 奈米粒子 ........................................................................... 11
2.4 染料樣品製備與檢測步驟 ..................................................................................... 12
2.4.1 標準樣品配製 ............................................................................................... 12
2.4.2 真實水樣配製與檢測步驟 ........................................................................... 12
三、結果與討論 ............................................................................................................ 13
3.1 PSS-Fe3O4 奈米粒子之性質探討 ........................................................................... 13
3.2 最佳化反應條件之探討 ......................................................................................... 20
3.2.1 PSS-Fe3O4 奈米粒子吸附能力探討 ............................................................ 20
3.2.2 吸附時間最佳化探討 ................................................................................... 20
3.2.3 PSS-Fe3O4 奈米粒子—Fenton 氧化法降解反應之過氧化氫濃度最適化 21
3.3 PSS-Fe3O4 與 Fe3O4 奈米粒子降解能力比較 ..................................................... 25
3.4 降解染料亞甲基藍與羅丹明B 的反應 ............................................................... 27
3.5 使用自來水樣品之降解染料反應 ......................................................................... 29
3.6 PSS-Fe3O4 NPs 降解染料反應之重複使用性探討 ............................................... 31
四、結論 ........................................................................................................................ 33
五、參考文獻 ................................................................................................................ 34
第二章利用磁性氧化鐵奈米粒子結合三磷酸腺苷螢光試劑檢測三磷酸腺苷 ...... 39
一、前言 ........................................................................................................................ 39
1.1 三磷酸腺苷（Adenosine triphosphate，ATP） 簡介 ........................................ 39
1.2 偵測方法—螢光消光 ............................................................................................. 40
二、實驗部分 ................................................................................................................ 43
2.1 藥品與溶液配製 ..................................................................................................... 43
2.2 儀器裝置 ................................................................................................................. 45
2.3 合成磁性氧化鐵奈米粒子 (Bare Fe3O4 奈米粒子) ........................................... 46
2.4 ATP 回復 Fe3O4 奈米粒子與 ATP-FL 之系統消光之檢測步驟 ....................... 47
2.4.1 標準樣品配製 ................................................................................................ 47
2.4.2 真實血球樣品之ATP 檢測 .......................................................................... 47
三、結果與討論 ............................................................................................................ 48
3.1 利用磁性氧化鐵奈米粒子結合三磷酸腺苷螢光試劑檢測三磷酸腺苷 ............. 48
3.2 最佳化條件探討 ..................................................................................................... 50
3.2.1 反應 pH 值探討 .......................................................................................... 50
3.2.2 緩衝溶液種類探討 ....................................................................................... 50
3.2.3 緩衝溶液濃度探討 ....................................................................................... 54
3.2.4 Fe3O4 奈米粒子用量探討 ............................................................................ 54
3.2.5 反應時間探討 ............................................................................................... 57
3.2.6 ATP-FL 螢光試劑濃度探討 ......................................................................... 57
3.3 消光機構探討 ......................................................................................................... 60
3.4 選擇性探討 ............................................................................................................. 62
3.5 ATP 標準品定量 ..................................................................................................... 64
3.6 血球樣品中 ATP 濃度定量 .................................................................................. 64
四、結論 ........................................................................................................................ 67
五、參考文獻 ................................................................................................................ 68

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