花鰍屬(Cobitis)魚類為生活於完全淡水之底棲性魚類，在台灣地區，中華花鰍普遍分布在中央山脈以西的水系，以及東半部的宜蘭縣、台北縣的水系中。台灣與中國南部地區之花鰍被認為是同一物種，即中華花鰍(Cobitis sinensis)，此為Sauvage和Dabry de Thiersant於1874年以四川揚子江之花鰍所發表的種名。花鰍屬只有少數的形態鑑別特徵可供區別，因此本研究除了詳細檢視外型特徵鑑定物種分類，再輔以DNA分子資訊重建親緣關係作分析則更顯重要。此外另再比較台灣地區淡水河流域與高屏溪流域之中華花鰍的生殖生態，觀察生殖模式的相關性。
由外部形態觀察及mtDNA D-loop序列的分析結果，可將台灣地區之中華花鰍種群及海峽鄰近大陸地區中華花鰍分為三個類群，即為 (1) 台灣西部與北部群 (NT) (Cobitis sinensis) (2) 台灣南部群 (ST) (C. sp1.) (3) 福建南部群 (SF) (C. sp2.)。研究中發現雄魚胸鰭骨板有相當明顯的種別分化，NJ tree與MP tree的分子類源關係樹狀圖所支持的支系群是一致的，顯示此樹狀圖結構應是整體趨勢之類源關係樹狀圖。
台灣南部水系花鰍生殖生態部分的研究發現，高屏溪之花鰍(C. sp1.) GSI指數於五月份時開始上升，九月底時下降，高峰期為五個月，上升趨勢約比溫度開始上升的三月份較晚，比雨季開始的七月份早。生殖腺成熟與卵徑分布皆與GSI指數有同步變化。

Chinese spined loach, Cobitis sinensis complex are primary benthic fishwater fishes with inhabiting on the substratum of small pebbles or coarse sand in mountain streams. In Taiwan, C. sinensis complex has been recorded from most drainages of western side of the Central Mountain Ridge including from Ilan, Taipei, even to Pingtung County. In Taiwan, all Cobitis populations have been considered as C. sinensis which types assigned from the Szechuan province, Yangtsi River basin by Sauvage and Dabry de Thiersant (1874). Due to very limited diagnostic morphological features can be detected and studied in detail, it’s more important for reconstructing the molecular phylogenetic relationship by DNA sequences. We also study in the reproductive biology by the samples in Kao-ping River, and to compare the realationship by the samples in Tan-shuei River.
By morphological analysis and mtDNA D-loop sequences clustering, we can recognize the C. sinensis complex of Taiwan and southern China into three group: (1) northern and middle Taiwan (NT) (as typical C. sinensis); (2) three River basins in southern Taiwan (ST) (C. sp1); (3) other samples from the Fujian provinces (ST) (C. sp2). They can be distinguished by very distinct differentiations of the scale of Canestrini. The type of NJ tree and MP tree are the overall same topology, mean that the trees of result are the stable phylogenetic tree of the whole C. sinensis complex.
By the result of reproductive biological study, GSI index rise in May and drop off in September, the high peak time is close to five months. The increasing tendency of GSI is later than the temperature rising, but earlier than the rainfall begin in July. They share the similar trend among the comparison of the mature of gonad、the distribution of egg diameter and GSI index.

謝辭………………………………………………………………………… i
中文摘要…………………………………………………………………… ii
英文摘要…………………………………………………………………… iv
目錄………………………………………………………………………… vi
圖目錄……………………………………………………………………… vii
表目錄……………………………………………………………………… viii
附錄目錄…………………………………………………………………… ix
壹、前言…………………………………………………………………… 1
貳、研究材料與方法……………………………………………………… 5
參、結果…………………………………………………………………… 11
肆、結論…………………………………………………………………… 25
伍、討倫…………………………………………………………………… 26
參考文獻…………………………………………………………………… 32
附圖………………………………………………………………………… 41
附表………………………………………………………………………… 59
附錄………………………………………………………………………… 66
個人履歷…………………………………………………………………….76

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