本研究主要是要探討多元胺 ，吲哚乙酸和激勃素對蘿蔔( Raphanus sativus L. cv. Luh Chin) 根伸長生長之效應。將蘿蔔的種苗外加spermidine 或spermine 0.01 mM培養一小時後，再加清水培養24小時，其根部的伸長量比外加0.1、1 或2 mM的結果較長。當以1 mM的spermidine 或spermine 處理5分鐘時，其結果則比對照組長，但是隨著處理時間增加，根伸長量卻減少了。putrescine以相同的處理條件卻沒有得到明顯效果。利用0.01、0.1、1、2 mM的spermidine抑制劑 ( cyclohexylamine ) 外加處理則都會抑制蘿蔔根之生長，抑制的效果和cyclohexylamine的劑量成正比。當同時外加1 mM spermine及IAA時，是在IAA10‾¹ × 6 nM的根伸長較佳，相對於spermine 加 IAA 10‾² × 6 nM或 1× 6 nM，但是皆比外加1mM spermine效果好，另外同時外加1 mM spermidine及IAA則是10‾² × 6 nM比IAA 10‾¹× 6 nM 或 1× 6 nM根之伸長還多，而同時外加spermidine或spermine及GA3均是在GA3 10‾²× 3 μM有最佳根伸長生長之效果，然而此處的任何處理還是都比對照組根之長度短，因此，不管是IAA或GA3均無法恢復根被spermine或spermidine抑制的結果。內生性游離態spermidine和spermine的分析結果，同時外加spermine和GA3的內生性spermidine和spermine含量則是隨著GA3濃度升高，有上升趨勢。同時加spermine及IAA則是在IAA10‾¹ × 6 nM得到最少的內生性spermine和spermidine。同時外加spermidine及IAA或GA3所得到的內生性spermidine並沒有明顯差異，僅在GA3 10‾²×3 μM有較高的spermidine，但是和外加1mM spermidine的內生性spermidine含量比較，則均為較低之結果。然而同時外加spermidine及IAA或GA3所得到的spermine則無明顯差異。

The effects of polyamines, indole-3-acetic acid ( IAA ) and gibberellic acid ( GA3 ) on root elongation in radish ( Raphanus sativus L. cv. Luh Chin ) were studied. Incubation of radish seedlings in spermine or spermidine at 0.01 mM for 1hour, and then transferred to deionized water for 24 hours at 25℃ in the dark promoted root elongation as compared with concentration at 0.1 or 2 mM. When roots were treated with spermine or spermidine at 1 mM for 5 minutes, and then transferred to deionized water for 24 hours, root length increased significantly compared with controls. However, root length reduced gradually with increasing treatment times. Putrescine did not affect root elongation when treated in the same manner as spermine or spermidine. Exogenous spermidine synthesis inhibitor ( cyclohexylamine ) at 0.01, 0.1, 1 or 2 mM to the roots inhibited root elongation. The inhibition of root elongation was parallel to cyclohexylamine doses. Root length increased when spermine at 1 mM plus IAA at 10‾¹ × 6 nM was applied for 1 hour, and then transferred to deionized water for 24 hours compared with spermine at 1 mM plus IAA 10‾² or 1× 6 nM. Root length was longer when treated with spermidine at 1 mM plus IAA at 10‾²× 6 nM than at 10‾¹ or 1× 6 nM. Root treated with spermine at 1 mM plus GA3 at 10‾²×3 μM Resulted in a longer root than treated with spermine at 1 mM plus GA3 at 10‾¹ or 1× 3 μM. Roots treated with spermidine at 1 mM plus GA3 at 10‾²×3 μM promoted root elongation. However, any treatments of spermine or spermidine in combination with IAA or GA3 significantly reduced the root length when compared with controls. Furthermore, either IAA or GA3 could not restore the inhibitory effects of root elongation caused by spermine or spermidine treatment at 1 mM for 1 hour and then transferred to deionized water for 24 hours. Endogenous spermidine and spermine contents after exogenous spermine plus GA3 treatment increased by increasing GA3 concentrations. But endogenous spermidine and spermine contents was the least in spermine 1 mM plus IAA 10‾¹ × 6 nM treatment. However, endogenous spermidine contents after exogenous spermidine plus IAA or GA3 application reduced significantly when compared with controls. But there is no significant difference of spermidine content between different exogenous IAA doses. In contrast, spermidine content maintained at a high level in spermidine at 1 mM plus GA3 at 10‾²×3 μM as compared with other spermidine plus GA3 combinations. However, endogenous spermine contents were not affected by exogenous spermidine plus IAA or GA3.

縮寫表………………………………………………………………3
中文摘要……………………………………………………………4
英文摘要……………………………………………………………6
前言…………………………………………………………………8
材料方法……………………………………………………………12
結果…………………………………………………………………19
討論…………………………………………………………………23
圖表…………………………………………………………………29
參考文獻……………………………………………………………42

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