Title page for etd-0206106-214758


[Back to Results | New Search]

URN etd-0206106-214758
Author HUI-JEN MIN
Author's Email Address n922050005@student.nsysu.edu.tw
Statistics This thesis had been viewed 5221 times. Download 6892 times.
Department Institute of Biomedical Sciences
Year 2005
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Microglial conditioned medium inhibited the dopamine- and Zn2+- induced PC12 cell death
Date of Defense 2006-01-16
Page Count 49
Keyword
  • zinc
  • parkinson's disease
  • conditioned medium
  • microglia
  • dopamine
  • Abstract Microglia have the potential to produce specific neurotrophic molecules in response to injury and brain diseases. Activated microglia are seen after brain injury or in neurological disease, such as Parkinson’s disease (PD). PD is a progressive neurodegenerative disorder. Although its cause remains unknown, it is believed that enhanced oxidative stress is a major component in the pathogenesis of nigral cell death in PD. Previous results have shown that DA induces apoptosis of dopaminergic neurons in a time- and concentration- dependent manner. In addition, a number of studies have shown that Zn2+ may enter the cell to reach toxic concentrations and that Zn2+ concentration is higher in the striatum of the postmortem brains of PD patients than that of the control brains. We have previously shown that Zn2+ synergistically enhanced the dopamine- and H2O2- induced PC12 cell death. To study the role of microglia in the cell death, I have examined the effect of conditioned medium from a human microglia cell line on the PC12 cell death induced by dopamine and Zn2+. The result shows that conditioned medium inhibits the PC12 cell death and the phosphorylation of JNK induced by dopamine and Zn2+ is diminished by the conditioned medium. It appears that the factor(s) that are responsible for the protection is heat-stable because the conditioned medium heated in 70℃for 30 minutes still has the ability to protect the cell death. Cell death induced by A23187 and C2-ceramide, but not by staurosporine can be protected by the conditioned medium. Results from this study suggest that the microglia secrete some factors which can protect neuron.
    Advisory Committee
  • Wen-Chun Hung - chair
  • Long-Sen Chang - co-chair
  • Ching-Jiunn Tseng - advisor
  • Lung-Sen Kao - advisor
  • Files
  • etd-0206106-214758.pdf
  • indicate access worldwide
    Date of Submission 2006-02-06

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have more questions or technical problems, please contact eThesys