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論文名稱 Title |
放養密度、內置物及飼料對白蝦不換水養殖之影響 Effect of different density, inbuilt objects and feeds on white shrimp (Litopenaeus vannamei) cultured in zero-exchange system |
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系所名稱 Department |
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畢業學年期 Year, semester |
語文別 Language |
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學位類別 Degree |
頁數 Number of pages |
75 |
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研究生 Author |
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指導教授 Advisor |
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召集委員 Convenor |
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口試委員 Advisory Committee |
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口試日期 Date of Exam |
2006-01-21 |
繳交日期 Date of Submission |
2006-02-07 |
關鍵字 Keywords |
白蝦、密度、內置物、不換水 density, Litopenaeus vannamei, zero-exchange system |
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統計 Statistics |
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中文摘要 |
不換水養殖系統因為不會任意的將富含營養鹽的養殖池水排出至環境中而造成環境的污染,且氮源可在池子中循環讓蝦子再利用,也可以降低換水所需的成本,減少抽取地下水,避免地層下陷的發生,故為一種可開發的養殖方式。但池水中的氨氮和亞硝酸濃度則因餵食高蛋白含量的飼料,會隨養殖時間的增加而累積增加,一般傳統養殖的解決方式就是換水,而不換水養殖系統則需利用硝化細菌來將毒性較高的氨氮及亞硝酸轉變為毒性較低的硝酸。 本報告在2噸FRP桶中以不換水的方式進行白蝦養殖試驗,並以供給充分的打氣使顆粒性有機物懸浮於水體中。試驗共分四個部分(1)內置物試驗:放置網套、網袋、掛網、珊瑚砂和無放置對照組。(2)密度試驗:100、200、300和400尾/m2。(3)飼料試驗:白蝦(protein 39%)、草蝦(protein 39%)和斑節蝦飼料(protein 54%)。(4)不換水及少量換水(35%池水/週)試驗。 試驗結果發現,內置物試驗中以網袋組有最大的白蝦產量、均重及最佳的飼料轉換率,但網套可以隔離最多的懸浮顆粒。白蝦平均體重隨密度的增加而降低,超過200尾/m2飼養密度後生長緩慢,以100尾/m2密度來飼養,雖可得到最大的白蝦平均體重,但以產量看來200尾/m2為最佳的飼養密度。飼料中含有較高的蛋白質,可提高蝦子對飼料的使用率,但高蛋白含量飼料的成本較高,如僅以蛋白質含量39%的草蝦飼料來餵養,一樣可以達到高產量。以不換水和少量換水兩試驗來做比較後發現,不換水試驗的水質條件,到試驗結束後,僅磷酸的濃度明顯較高,其他氨氮、亞硝酸及硝酸皆無明顯差異,故不換水養殖在水質方面亦可達到換水養殖的水準,最後存活率、平均體重及FCR和換水組無異,且得到比換水養殖更高的產量。 本研究最後歸納得知不換水系統中以密度200尾/m2來養殖白蝦,利用內置網袋及投餵蛋白質含量39%的草蝦飼料,為最佳的不換水養殖組合。 |
Abstract |
Zero-exchange system doesn’t discharge the nutrient-rich pool water to the environment, so it doesn’t cause the pollution. Besides, nutrients in the water can be re-used and the amount of water used can be reduced. In shrimp pond, ammonia and nitrite will accumulate during the period of cultivation because of feeding large amount of protein-rich feed. In traditional culture system, a great deal of water was used to solve the problem. In zero-exchange system, nitro-bacteria were used to convert ammonia and nitrite into nitrate, the need of water exchange was much-reduced. In this study, shrimps (Litopenaeus vannamei) were cultured in FRP tanks, and organic particles were suspended by strong aeration. There are four experiments (1) substrate: net-liners, net-pockets, mats, coral granules and blank control. (2) density: 100, 200, 300 and 400 /m2. (3) feeds: White shrimp feed (contain 39% protein), tiger shrimp feed (contain 39% protein) and Kuruma prawn feed (contain protein 54%). (4) zero-exchange water and change water (35% ponds of water / week). Net-pockets have the highest yield and final weigh and lowest FCR, and the net-liners isolate highest amount of the suspending particle. Shrimp final weight reduces as the stocking density increases, white shrimp grows slower when density excess 200 /m2. Feed contains higher protein can improve the efficiency of feed used by shrimp, but the cost of the feed become higher. Use the tiger shrimp feed (containing 39% protein) only can also reach high yield. Except orthophosphate, ammonia, nitrite and nitrate are not different from the exchange system. When compared with those of the zero-exchange system survival, final weigh and FCR are not different between two the treatments, but yield is higher in zero-exchange system. In conclusion, shrimp cultured in zero-exchange system with density of 200 /m2, substrate of net-pockets and feeds the contain 39% protein can reveal optimum yield. |
目次 Table of Contents |
中文摘要------------------------------------------------------------------------------------------I 英文摘要----------------------------------------------------------------------------------------III 總目錄--------------------------------------------------------------------------------------------V 圖目錄-------------------------------------------------------------------------------------------VI 表目錄------------------------------------------------------------------------------------------VII 壹、前言-------------------------------------------------------------------------------------------1 貳、材料方法-------------------------------------------------------------------------------------6 2.1 不同內置物對白蝦不換水養殖的影響-------------------------------------------------6 2.2不同飼養密度對白蝦不換水養殖的影響-----------------------------------------------7 2.3不同飼料蛋白質含量對白蝦不換水養殖的影響--------------------------------------8 2.4比較換水和不換水對白蝦養成的影響--------------------------------------------------8 2.5水質檢測方法--------------------------------------------------------------------------------9 2.6飼料檢測方法------------------------------------------------------------------------------13 參、結果 3.1不同內置物對白蝦不換水養殖的影響之結果---------------------------------------16 3.2不同飼養密度對白蝦不換水養殖的影響之結果------------------------------------18 3.3不同飼料蛋白質含量對白蝦不換水養殖的影響之結果---------------------------20 3.4比較換水和不換水對白蝦養成的影響之結果---------------------------------------22 肆、討論------------------------------------------------------------------------------------------24 4.1不同內置物對白蝦不換水養殖的影響之討論---------------------------------------24 4.2不同飼養密度對白蝦不換水養殖的影響之討論------------------------------------26 4.3不同飼料蛋白質含量對白蝦不換水養殖的影響之討論---------------------------27 4.4比較換水和不換水對白蝦養成的影響之討論---------------------------------------28 伍、參考文獻------------------------------------------------------------------------------------54 陸、附錄------------------------------------------------------------------------------------------63 |
參考文獻 References |
丁雲源,江英智,吳俊宗,1995。養殖用水循環利用技術資料輯。農委會漁業特 刊第五十一號。 王世經,張銘智,2000。超集約室外循環水系統養殖日本鰻。生物資源生物技術 2,51-56。 中華民國行政院環境保護署環境檢驗所,2005。硝酸(水中硝酸鹽氮檢測方法- 分光光度計法,NIEA W419.50A)。總懸浮固體、總固體(水中總溶解固體及 懸浮固體檢測方法-103℃∼105℃乾燥,NIEA W210.56A)。 行政院農委會漁業署漁業統計漁業年報,1995-2002,2004。 吳峙翰,2002。白蝦後期幼蟲培育效率之改善。國立台灣海洋大學水產養殖學 系碩士學位論文。 張浚銘,2005。不換水白蝦養殖技術之建立。國立中山大學海洋生物研究所碩 士論文。 張碧兒,1996。不同蛋白質含量飼料對石斑魚及草蝦氮排泄之影響。國立台灣 海洋大學水產養殖系研究所碩士學位論文。 陳弘成,1999。白蝦養殖與管理方式。養魚世界 273,p 66-68。 陳秀男,沈士新,冉繁華,林清龍,王俊順,黎錦超,2000。蝦類養殖技術實 用大全。觀賞魚雜誌社出版。台北,台灣。 劉文御,1988。南美厄瓜多養殖白蝦池有害之初步調查報告。中國水產 431,9-18。 劉文御,蘇茂森,徐崇仁,1999。白蝦專輯 行政院農委會水產試驗所養蝦推廣 手冊。 盧民益,黃美瑩,張錦宜,張振元,劉文御,徐崇仁,2000。白蝦室內高密度養 殖初步試驗。養魚世界 285,69-77。 Avnimelech, Y., 1999. C/N ratio as a control element in aquaculture systems. Aquaculture 176, 227– 235. Avnimelech, Y., Diab, S., Kochva, M., Mokady, S., 1992. Control and utilization of inorganic nitrogen intensive fish culture ponds. Aquac. Fish. Manage. 23, 421–430. Avnimelech, Y., Kochva, M., Diab, S., 1994. Development of controlled intensive aquaculture systems with a limited water exchange and adjusted C to N ratio. Isr. J. Aquac.-Bamidgeh 46, 119– 131. Blackburn, T. H., Henriksen, K.,1983. Nitrogen cycling in different types of sediments from Danish water. Limnology Oceanography 28, 477-493. Boyd, C.E., Yoo, K.H., 1994. Hydrology andWater Supply for Pond Aquaculture. Chapman and Hall, New York,pp. 439–449. Bratvold, D., Lu, J., Browdy, C. L., 1999. Disinfection, Microbial community establishment and shrimp production in a prototype biosecure pond. Journal of the world aquaculture society 30, 422-432. Browdy, C.L., Bratvold, D., Stokes, A.D., McIntosh, R.P., 2001. Perspectives on the application of closed shrimp culture systems. In: Browdy, C.L., Jory, D.E. (Eds.), The New Wave, Proceedings of the Special Session on Sustainable Shrimp Culture, Aquaculture 2001. The World Aquaculture Society, Baton Rouge, USA, pp. 20–34. Burford, M.A., Williams, K.C., 2001. The fate of nitrogenous waste from shrimp feeding. Aquaculture 198 : 79-93. Burford, M.A., Thompson, P.J., McIntosh, R.P., Bauman, R.H., Pearson, D.C., 2003. Nutrient and microbial dynamics in high-intensity, zero-exchange shrimp ponds in Belize. Aquaculture 219, 393–411. Castille, Jr.F.L., Lawrence, A.L., 1981. The effect of salinity on the osmotic, sodium, and chloride concentrations in the hemolymph of euryhaline shrimp of the genus Penaeus. Comp. Biochem. Physiol. 68 , 75– 80. Chen, J.C., Chen, S.F., 1992. Effects of nitrite on growth and molting of Penaeus monodon juveniles. Comp. Biochem. Physiol. 101 , 453– 458. Chen, J.C., Chin, T.C., 1998. Joint action of ammonia and nitrite on tiger prawn, Penaeus monodon, postlarvae. J. World Aquacult. Soc. 19, 143–148. Chen, J.C., Kou, Y.Z., 1992. Effect of ammonia on growth and molting of Penaeus japonicus juveniles. Aquaculture 104, 249-260 Chen, J.C., Lei, S.C., 1990. Toxicity of ammonia and nitrite to Penaeus monodon juveniles. J. World Maricult. Soc. 21 , 300–306. Colt, J., Armstrong, D., 1981. Nitrogen toxicity to fish, crustaceans and molluscs. Bio-engineering Symposium for Fish Culture. American Fisheries Society, Bethesda, MD, pp. 34–47. Cowan, V.J., Lorenzen, K., Funge-Smith, S.J., 1999. Impact of culture intensity and monsoon season on water quality in Thai commercial shrimp ponds. Aquac. Res. 30, 123– 133. Cruz-Suarez, L.E., Antimo-Perez, J.S., Luna-Mendoza, N., Ricque-Marie, D., 2001. Effect of two different vegetable animal protein proportions on the optimum dietary protein/energy ratio for Book of Abstracts, World Aquaculture Society. P, 148. Davis, D.A., Arnoid, C.R., 1998. The design, management and production of a recirculating raceway system for the production of marine shrimp. Aquacultural Engineering 17, 193-211. Eng, C.T., Paw, J.N., Guarin, F.Y., 1989. The environmental impact of aquaculture and the effects of pollution on coastal aquaculture development in southeast Asia. Mar. Pollut. Bull. 20, 335– 343. Fast, W.A., Menasveta, P., 2000. Some recent issues and innovations in merine shrimp pond culture. Reviews in Fisheries science 8 , 151-233. Greenberg, A.E., Clesceri, L.S., Eaton, A.D., 1992. Standard Methods for The Examination of Water and Wastewater-4500-P E. Ascorbic Acid Method, p 4-115. Hepher, B., 1988. Nutrition of Pond Fish. Cambridge Univ. Press, Cambridge, UK, 388 pp. Hopkins, J.S., Hamilton, R.D., Sandifer, P.A., Browdy, C.L., Stokes, A.D., 1993. Effect of water exchange rate on production, water quality, effluent characteristics and nitrogen budgets of intensive shrimp ponds. J. World Aquacult. Soc. 24, 304–320. Hopkins, J.S., Sandifer, P.A., Browdy, C.L., 1995. Effect of two feed protein levels and feed rate combinations on water quality and production of intensive shrimp ponds operated without water exchange. J. World Aquacult. Soc. 26 , 93-97. Jackson, C., Preston, N., Thompson, P.J., Burford, M., 2003. Nitrogen budget and effluent nitrogen components at an intensive shrimp farm. Aquaculture 218, 397-411. Juliette, L.Y., Hyman, M.R., Arp, D.J., 1993. Inhibition of ammonia oxidation in Nitromonas europaea by sulfur compounds: thioethers are oxidized to sulfoxides by ammonia monooxygenase. Appl. Environ. Microbiol. 59, 3718–3722. Kautsky, N., Rönnbäck, P., Tedengren, M., Troell, M., 2000. Ecosystem perspectives on management of disease in shrimp pond farming. Aquaculture 191, 145– 161. Kureshy, N., Davis, D.A., 2002. Protein requirement for maintenance and maximum weight gain for the Pacific white shrimp,Litopenaeus vannamei. Aquaculture 204, 125-143. Liao, I.C.,1989. Penaeus monodon culture in Taiwan : through two decades of growth .Int. J. Aq. Fish. Technol. 1, 16-24. Lin, M.N., Ting, Y.Y., Tzeng, B.S., Liu, C.Y., 1989. Penaeid Parental Shrimp Rearing: Culture of the Third Generation in Penaeus vannamei. J. Fish. Soc. Taiwan. 17 , 125-132. Lin, Y.C., Chen, J.C., 2001. Acute toxicity of ammonia on Litopenaeus vannamei Boone Juveniles at different salinity levels. J. Exp. Mar. Biol. Ecol. 259,139-148. Lin, Y.C., Chen, J.C., 2003.Acute toxicity of nitrite on Litopenaeus vannamei (Boone) juveniles at different salinity levels. Aquaculture 224, 193–201. Martinez-Cordova, L.R., Villarreal-Colmenares, H., Porchas-Cornejo, M.A., Naranjo-Paramo, J., Aragon-Noriega, A., 1997. Effect of aeration rate on geowth, survival and yield of white shrimp Penaeus vannamei in low water exchange ponds. Aquacultural Engineering 16, 85-90. McIntosh, D., Samocha, T.M., Jones, E.R., Lawrence A.L., Horowitz S., Horowitz, A., 2001. Effects of two commercially available low-protein diets (21% and 31%) onwater and sediment quality, and on the production of Litopenaeus vannamei in an outdoor tank system with limited water discharge. Aquacultural Engineering 25, 69-82. Menz, A., Blake, B.F., 1980. Experiments on the growth of Penaeus vannamei Boone. J. Exp. Mar. Biol. Ecol. 48, 99–111. Moss, S.M., Divakaran, S., Kim, B.G., 2001. Stimulating effects of pond water on digestive enzyme activity in the Pacific white shrimp, Litopenaeus vannamei (Boone). Aquaculture 199,311-321. Moss, S.A., Pruder, G.D., 1995.Characterization of organic particles associated with rapid growth in juvenile white shrimp, Penaeus vannamei Boone, reared under intensive culture conditions. J. Exp Mar. Biol. Ecol. 187, 175-191. Moss, S.A., Pruder, G.D., Samocha, T.M., 1999. Environmental management and control: controlled ecosystem and biosecure shrimp growout systems. In: Bullis, R.A., Pruder, G.D. (Eds.), Controlled and Biosecure Production Systems, Preliminary Proceedings of a Special Integration of Shrimp and Chicken Models, 27–30 April. Sydney, Australia. World Aquaculture Society. pp, 87–91. Moss, S.M., Reynolds, W.J., Mahler, L.E., 1998. Design and Economic Analysis of a Protogype Biosecure Shrimp Growout Facility. In: Moss, S.M. (Ed.), Proceedings of the US Marine Shrimp Farming Program Biosecurity Workshop, 14 February 1998, Hawaii, United States. The Oceanic Institute, Hawaii, United States. pp, 5–14. Naylor, R.L., Goldburg, R.J., Mooney, H., Beveridge, M., Clay, J., Folke, C., Kautsky, N., Lubchenco, J., Primavera, J., Williams, M., 1998. Nature’s subsidies to shrimp and salmon farming. Science. 282, 883–884. Parry, G., 1960 . Excretion In: Waterman, T.H. (Editor), The physiology of Crustacea. Academic press. New York. I, 341-366. Parsons, T.R., Maita, Y., Lalli, C.M., 1984. A Manual of Chemical and Biological Methods for Seawater Analysis-Ditermination of Nitrite, p7~p9. Ditermination of Ammonia (Alternative Method), p14~17. Penaflorida, V. D., 1999. Interaction between dietary levels of calcium and phosphorus on growth of juvenile shrimp, Penaeus monodon. Aquaculture 172: 281–289 Pérez Farfante, I., Kensley, B., 1997. Penaeid and sergestoid shrimps and prawns of the world: keys and diagnoses. Mémoires du Muséum National D’Histoire Naturelle, Paris. 233 pp. Ponce-Palafox, J., Martinez-Palacios, C.A., Ross, L.G., 1997. The effect of salinity and temperature on the growth and survival rates of juvenile white shrimp, Penaeus vannamei Boone, 1931. Aquaculture 157, 107–115. Primavera, J.H., 1998. Tropical shrimp farming and its sustainability. Page 257-289 in S. S. De Silva, editors. Tropical Mariculture. Academic Press, San Diego. Pruder, G.D., 2004. Biosecurity: application in aquaculture. Aquacultural Engineering 32, 3–10. Regnault, M., 1987. Nitrite excretion in marine and fresh-water crustacean. Biol. Rev., 62, 1-24. Sandifer, P.A., Hopkins, J.S., 1996. Conceptual design of a sustainable pond-based shrimp culture system. Aquacultural Engineering 15, 41– 52. Schuur, A.M., 2003.Evaluation of biosecurity applications for intensive shrimp farming. Aquacultural Engineering 28, 3 -20. Taiwan Fisheries Bureau (1987). Fisheries Yearbook of Taiwan Area. Department of Agriculture and Forestry, Provincial Government of Taiwan, 236pp. (in Chinese) Thakur, D.P., Lin, C.K., 2003. Water quality and nutrient budget in closed shrimp (Penaeus monodon) culture systems. Aquacultural Engineering 27, 159–176. Timmons, M.B., Losordo, T.M., 1994. Aquaculture Water Reuse Systems: Engineering Design and Management. Elsevier, Amsterdam. William, S. 1984. Official methods of analysis of the Association of Official Analytical Chemists. (AOAC). |
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