論文使用權限 Thesis access permission:校內公開,校外永不公開 restricted
開放時間 Available:
校內 Campus: 已公開 available
校外 Off-campus:永不公開 not available
論文名稱 Title |
大豆下胚軸不定根誘發過程中過氧化同功酶的表現 Differential responses of peroxidases during the formation of adventitious root in hypocotyl cuttings of soybean |
||
系所名稱 Department |
|||
畢業學年期 Year, semester |
語文別 Language |
||
學位類別 Degree |
頁數 Number of pages |
42 |
|
研究生 Author |
|||
指導教授 Advisor |
|||
召集委員 Convenor |
|||
口試委員 Advisory Committee |
|||
口試日期 Date of Exam |
2009-01-13 |
繳交日期 Date of Submission |
2009-02-09 |
關鍵字 Keywords |
不定根、生長素、大豆、過氧化酶 adventitious root, Glycine max, auxin, peroxidase |
||
統計 Statistics |
本論文已被瀏覽 5688 次,被下載 4 次 The thesis/dissertation has been browsed 5688 times, has been downloaded 4 times. |
中文摘要 |
外加植物生長素(IAA, IBA, NAA)可以促進大豆下胚軸誘發不定根,不同的植物生長素促進發根的能力也有所不同,其中又以Indole-3-butyric acid (IBA)促進大豆下胚軸誘發不定根的能力最好。在不定根發育的誘導期(induction phase),外加的生長素會抑制陰性(pI 3.7) 和陽性 (pI 8.5) 過氧化酶活性的表現。而在不定根發育的末期,在外加IBA的大豆下胚軸中,pI 5.3過氧化酶活性會大幅表現,同時,過氧化氫的含量也有明顯上升的趨勢。之前的研究發現pI 8.5過氧化酶基因的啟動子含有兩個auxin response element (ARF/AuxRE和CATATGGMSAUR)。我的研究則是發現在pI 5.3 過氧化酶基因啟動子有一個auxin response element (ARF/AuxRE)。因此推測pI 5.3 過氧化酶可能和不定根形成的末期有關。pI 5.3 過氧化酶會藉由與auxin的結合產生過氧化氫,並調控auxin的訊息傳遞路徑來影響植物的生長。 |
Abstract |
The auxins, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA), promotes the formation of adventitious root in hypocotyls of soybean (Glycine max). IBA significantly promotes the formation of adventitious roots more than IAA and NAA . The activity of anionic pI 3.7 peroxidase (POX) and cationic pI 8.5 POX were inhibited by exogenous auxins during the induction of adventitious root on day 2. Besides, the activity of pI 5.3 POX was enhanced by IBA during the initiation stage on day 4. The increase of the activity of pI 5.3 POX was accompanied by the increase of H2O2 levels. In the previous researches, it shows that the promoter of pI 8.5 POX gene contains both ARF/AuxRE and CATATGGMSAUR motifs that are responded to auxins. In this studies, the pI 5.3 POX gene, which responses to auxins a day or two later, contains only ARF/AuxRE motif. The regulation of pI 5.3 POX gene is probably initiation phase-dependent. The results suggest that anionic pI 5.3 POX produces significant amount of H2O2 through the binding of auxin to POX and mediate the auxin signaling pathway leading to plant growth . |
目次 Table of Contents |
Chinese abstract……………………………………….2 English abstract………………………………………..3 Table of contents………………………………….……4 List of tables and figures………………………………5 Introduction…………………………………...………..6 Materials and methods……………………..…………10 Results………………………………………..………...16 Discussion……………………………………..………..19 References………………………………….…………...24 Tables and figures……………………………………...33 |
參考文獻 References |
Aeschbacher RA, Schiefelbein JW and Benfey PN(1994) The genetic and molecular basis of root development. Annu Rev Plant Physiol. Plant Mol. Biol. 45,25-45 Bailey-Serres J and Mittler R (2006) The Roles of Reactive Oxygen Species in Plant Cells. Plant Physiol. 141, 311 Berleth T and Sachs T (2001) Plant morphogenesis: long-distance coordination and local patterning. Curr. Opin. Plant Biol. 4, 57-62 Bolwell GP, Bindschedler LV, Blee KA, Butt VS, Davies DR, Gardner SL, Gerrish C and Minibayeva F (2002) The apoplastic oxidative burst in response to biotic stress in plants: a three-component system. J. Exp. Bot. 53, 1367-1376 Carpin S, Crevecoeur M, Greppin H and Penel C (1999) Molecular cloning and tissue-specific expression of an anionic peroxidase in zucchini. Plant Physiol. 120, 799-810 Chao IL, Cho CL, Chen LM, Liu ZH (2001) Effect of indole-3-butyric acid on the endogenous indole-3-acetic acid and lignin contents in soybean hypocotyl during adventitious root formation. J. Plant Physiol. 158, 1257-1262 Chen LM, Cheng JT, Chen EL, Yiu TJ and Liu ZH (2002) Naphthaleneacetic acid suppresses peroxidase activity during the induction of adventitious roots in soybean hypocotyls. J. Plant Physiol. 159, 1349-1354 Chen S and Schopfer P (1999) Hydroxyl-radical production in physiological reactions : A novel function of peroxidase . Eur. J. Biochem. 260, 726-735 Chen Z, Silva H and Klessig DF (1993) Active oxygen species in the induction of plant systemic acquired resistance induced by salicylic acid. Sci. 262, 1883-1886 Chou CH (2009) Effect of auxins on peroxidase gene in hypocotyl cuttings of soybean during adventitious root formation. NSYSU Biological Sciences Master’s Thesis De Klerk GJ, Brugge JT and Marinova S (1997) Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’. Plant Cell, Tissue and Organ Cult. 49, 39–44 Dunand C, Meye MD, Crevecoeur M and Penel C (2003) Expression of a peroxidase gene in zucchini in relation with hypocotyl growth. Plant Physiol. and Bio. 41, 805–811 Duroux L, Welinder KG (2003) The peroxidase gene family in plants: a phylogenetic overview. J. Mol. Evol. 57, 397–407 Ermel FF, Vizoso S, Charpentier JP, Jay-Allemand C, Catesson AM and Couee I (2000) Mechanisms of primordium formation during adventitious root development from walnut cotyledon explants. Planta 211,563-574 Gazaryana IG, Chubara TA, Mareevaa EA, Lagriminib LM,Van Huysteec RB and Thorneleyd RNF (1999) Aerobic oxidation of indole-3-acetic acid catalysed by anionic and cationic peanut peroxidase. Phytochemistry 51, 175-186 Gendreau E, Traas J, Demos T, Grandjean O, Caboche M and Hofte H (1997) Cellular basis of hypocotyl growth in Arabidopsis thaliana. Plant Physiol. 114, 295-305 Giuliano G, Pichersky E, Malik VS, Timko MP, Scolnik PA and Cashmore AR (1988) An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. Proc. Natl. Acad. Sci. USA 85, 7089-7093 Gonzalez LF, Perez F and Rojas1 MC (1999) Indole-3-Acetic Acid Control on Acidic Oat Cell Wall Peroxidases. J. Plant Growth Regul. 18:25–31 Goldfarb B, Hackett WP, Furnier GR, Mohn CA and Plietzsch A (1998) Adventitious root initiation in hypocotyl and epicotyl cuttings of eastern white pine (Pinus strobus) seedlings. Physiol. Plant. 102, 513-522 Guilfoyle TJ and Hagen G (2007) Auxin response factors. Curr. Opin. Plant Biol. 10, 453–460 Hagen G and Guilfoyle TJ (2002) Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Mol. Biol. 49, 373–385 Hatzilazarou SP, Syrosa TD, Yupsanisb TA, Bosabalidisc AM and Economo AS (2006) peroxidases, lignin and anatomy during in vitro and ex vitro rooting of gardenia (Gardenia jasminoides Ellis) microshoots. J. Plant Physiol. 163, 827-836 Higo K, Ugawa Y, Iwamoto M and Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Res. 27, 297-300 Hiraga S, Sasaki K, Ito H, Ohashi Y and Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol. 42, 462-468 Hobbie LJ (1998) Auxin: Molecular genetic approaches in Arabidopsis Plant Physiol. Biochem. 36, 91-102 Jiang ZY, Woollard AC, Wolff SP (1990) Hydrogen peroxide production during experimental protein glycation. FEBS Lett 268, 69-71 Johri S, Jamwal U, Rasool S, Kumar A, Verma V and Qazi GN (2005) Purification and characterization of peroxidases from Withania somnifera (AGB 002) and their ability to oxidize IAA. Plant Sci. 169, 1014-1021 Kaur S, Cheema SS, Chhabra BR and Talwar KK (2002) Chemical induction of physiological changes during adventitious root formation and bud break in grapevine cuttings. Plant Growth Regul. 37, 63-68 Kawano T (2003) Role of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. Plant Cell Rep. 21, 829-937 Kim YH, Kim CY, SongWK, Park DS, Kwon SY, Lee HS, Bang JW and Kwak SS (2008) Overexpression of sweetpotato swpa4 peroxidase results in increased hydrogen peroxide production and enhances stress tolerance in tobacco. Planta 227, 867-881 Klotz KL and Lagrimini LM (1996) Phytohormone control of the tobacco anionic peroxidase promoter. Plant Mol. Biol. 31, 565-573 Kollarova K, Liskova D, Kakoniova D and Lux A (2004) Effect of auxins on Karwinskia humboldtiana root cultures. Plant Cell, Tissue and Organ Cult. 79, 213-22 Lagrimini LM (1996) in Plant Peroxidase : Biochemistry and Physiology, pp.235-242 Lagrimini LM and Rothstein S (1987) Tissue specificity of tobacoo peroxidase isozymes and their induction by wounding and tobacoo mosaic virus infection. Plant Physiol. 84, 438-442 Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y, Rouze P and Rombauts S (2002) PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res. 30, 325-327 Lindroth AM, Saarikoski P, Flygh G, Clapham D, Gronroos R, Thelander M, Ronne H and Arnold S von (2001) Two S- adenosylmethionine synthetase-encoding genes differentially expressed during adventitious root development in Pinus contorta. Plant Mol. Biol. 46, 335-346 Ljung K, Bhalerao RP and Sandberg G (2001) Sites and homeostatic control of auxin biosynthesis in Arabidopsis during vegetative growth. Plant J. 28(4), 465-474 Liszkay A, Kenk B, and Schopfer P (2003) Evidence for the involvement of cell wall peroxidase in the generation of hydroxyl radicals mediating extension growth. Planta 217, 658-667 Liu ZH, Hsiao IC and Pan YW (1996) Effect of naphthaleneacetic acid on endogenous indole-3-acetic acid, peroxidase and auxin oxidase in hypocotyl cuttings of soybean during root formation. Bot. Bull. Acad.Sin. 37, 247-253 Ludwig-Müller J (2000) Indole-3-butyric acid in plant growth and development. Plant Growth Regul. 32, 219-230 Ludwig-Müller J, Vertocnik A and Town CD (2005) Analysis of indole-3-butyric acid-induced adventitious root formation on Arabidopsis stem segments. J. Exp. Bot. 56, 2095-2105 McClure BA, Guilfoyle T (1989) Rapid redistribution of auxin- regulated RNAs during gravitropism. Science 243, 91-93 McDonald MP and Visser EJW (2003) A study of the interaction between auxin and ethylene in wild type and transgenic ethylene-insensitive tobacco during adventitious root formation induced by stagnant root zone conditions. Plant Boil. 5, 550-556 Miao Y, Lv D, Wang P, Wang XC, Chen J, Miao C and Songa CP (2006) An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses. Plant Cell 18, 2749-2766 Mika A, Minibayeva F, Beckett R and Luthje S (2004) Possible functions of extracellular peroxidases in stress-induced generation and detoxification of active oxygen species. Phytochem. Rev. 3, 173- 193 Nag S, Saha K and Choudhuri MA (2001) Role of auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rooting. J. Plant Growth Regul. 20, 182-194 Nordstrom AC, Jacobs FA and Eliasson L (1991) Effect of exogenous indole-3-acetic acid and indole-3-butyric acid on internal levels of the respectiv auxins and their conjugation with aspartic acid during adventitious root formation in pea cuttings. Plant Physiol. 96, 856-861 Pan R and Tian X (1999) Comparative effect of IBA, BSAA and 5,6-Cl2-IAA-Me on the rooting of hypocotyl in mung bean. Plant Growth Regul. 27, 91-98 Passardi F, Penel C and Dunand C (2004) Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci. 9, 534-540 Passardi F, Cosio C, Penel C and Dunand C (2005) Peroxidases have more functions than a Swiss army knife. Plant Cell Rep. 24, 255-265 Passardi F, Tognolli M , Meyer M De, Penel C and Dunand C (2006) Two cell wall associated peroxidases from Arabidopsis influence root elongation. Planta 223, 965-974 Redman J, Whitcraft J, Johnson C and Arias J (2002) Abiotic and biotic stress differentially stimulate as-1 element activity in Arabidopsis. Plant Cell Rep. 21, 180-185 Rival A, Bernard F and Mathieu Y (1997) Changes in peroxidase activity during in vitro rooting of oil palm (Elaeis guineensis Jacq.). Scientia Horticulturae 71, 103-112 Rombauts S, Dehais P, Montagu MV and Rouz P (1999) PlantCARE, a plant cis-acting regulatory element database. Nucleic Acids Res. 27, 295-296 Rout GR, Samantaray1 S and Das P (2000) In vitro rooting of Psoralea corylifolia Linn: Peroxidase activity as a marker. Plant Growth Regul. 30, 215-219 Rout GR (2006) Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) Kuntze and associated biochemical changes. Plant Growth Regul. 48, 111-117 Savitsky PA, Gazaryan IG, Tishkov VI, Lagrimini LM, Ruzgas T and Gorton L (1999) Oxidation of indole-3-acetic acid by dioxygen catalysed by plant peroxidases : specificity for the enzyme structure. Biochem. J. 340, 579-583 Schopfer P (2001) Hydroxyl radical-induced cell-wall loosening in vitro and in vivo: implications for the control of elongation growth. The Plant J. 28, 679–688 Schopfer P, Liszkay A, Bechtold M, Frahry G and Wagner (2002) Evidence that hydroxyl radicals mediate auxin-induced extension growth. Planta 214, 821–828 Schopfer P, Liszkay A (2006) Plasma membrane- generated reactive oxygen intermediates and their role in cell growth of plants. BioFactors. 28, 73-81 Sedira M (2006) Studies of Adventitious Root Formation in Woody Species. Swedish University of Agricultural Sciences Doctoral thesis Simonovicova M, Huttova J , Mistrık B, Siroka B and Tamas L (2004) Peroxidase mediated hydrogen peroxide production in barley roots grown under stress conditions. Plant Growth Regul. 44, 267-275 Sorin C, Bussell JD, Camus I, Ljung K, Kowalczyk M, Geiss G, McKhann H, Garcion C, Vaucheret H, Snadberg G and Bellini C (2005) auxin and light control of adventitious rooting in Arabidopsis require ARGONAUTE1. Plant cell 17, 1343-1359 Syros T, Yupsanis Y, Zafiriadis H and Economou A (2004) Activity and isoforms of peroxidases, lignin and anatomy, during adventitious rooting in cuttings of Ebenus creticaL. J. Plant Physiol. 161, 69-77 Tognolli M, Penel C, Greppin H and Simon P (2002) Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana. Gene 288, 129-138 Ulmasov T, Liu ZB, Hagen G and Guilfoyle TJ (1995) Composite structure of auxin response elements. Plant Cell 7, 1611–1623 Ulmasov T, Murfett J, Hagen G and Guilfoyle TJ (1997) Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell 9, 1963–1971 Valerio L, De Meyer M, Penel C and Dunand C (2004) Expression analysis of the Arabidopsis peroxidase multigenic family. Phytochemistry 65(10), 1331-1342 Vatulescu AD, Fortunato AS, Sa MC, Amancio S, Ricardo CPP and Jackson PA (2004) Cloning and characterisation of a basic IAA oxidase associated with root induction in Vitis vinifera. Plant Physiol. and Biochem. 42, 609-615 Welinder KG (1992) Plant peroxidases: structure-function relationship. In Plant Peroxidases (Penel, C. et al., eds), pp.1-24, University of Geneva, Switzerland. |
電子全文 Fulltext |
本電子全文僅授權使用者為學術研究之目的,進行個人非營利性質之檢索、閱讀、列印。請遵守中華民國著作權法之相關規定,切勿任意重製、散佈、改作、轉貼、播送,以免觸法。 論文使用權限 Thesis access permission:校內公開,校外永不公開 restricted 開放時間 Available: 校內 Campus: 已公開 available 校外 Off-campus:永不公開 not available 您的 IP(校外) 位址是 18.217.60.35 論文開放下載的時間是 校外不公開 Your IP address is 18.217.60.35 This thesis will be available to you on Indicate off-campus access is not available. |
紙本論文 Printed copies |
紙本論文的公開資訊在102學年度以後相對較為完整。如果需要查詢101學年度以前的紙本論文公開資訊,請聯繫圖資處紙本論文服務櫃台。如有不便之處敬請見諒。 開放時間 available 已公開 available |
QR Code |