螢光奈米鑽石(FND ,fluorescent nano-diamond)為一種性質優秀的螢光標定材料，與染料分子與螢光蛋白相比其擁有較佳的光穩定性，與量子點相比其對於生物沒有毒性，FND內由兩個氮與一個空缺(vacancy)結合而成的結構被稱為H3缺陷結構，可發出綠光所以被稱為gFND，而製作gFND需要較高含量不純物氮的鑽石，因此也容易產生其他的缺陷結構，影響H3缺陷結構的發光效率。本論文中，想要利用光譜的方法來分析並瞭解H3缺陷結構的發光行為，包括使用螢光與散射光影像來瞭解個別鑽石顆粒發光的均勻性，以螢光光譜來取得所有可發光缺陷結構的螢光強度，進而了解鑽石顆粒中所含的不同缺陷結構的比例，並以螢光生命期的測量來瞭解H3缺陷結構的發光行為。

Fluorescent nano-diamond(FND) is an unique fluorescence bio-labeling materials, which exhibit good fluorescence yield, excellent photostability, and non-toxicity. The emission color of FND is determined by the defect centers in the diamond crystal. When the defect center composed of one vacancy and two nearest-neighborhood nitrogen substitutes, it forms a H3 center. H3 center has a zero-phonon line at 496nm , and a broadband green emission around 530 nm,. When the FND contains lots of H3 centers, the emission color is green, hence it’s called green FND(gFND).
Since H3 centers composed of two nitrogen substitutes, it is naturally to fabricate the gFNDs by diamonds with high nitrogen substitutes. However, H3 center is not the only products when the diamond contains many nitrogen substitutes, and high density of vacancies. Other type of defect centers (NV-, NV0, …) exhibit lower energy gap, and quench the emission of H3 centers.
In this thesis, it aims to study the spectroscopic homogeneity of the gFNDs. Comparing the intensity of the scattering images and the corresponding fluorescence images, it provides the information of the relation between particle size and the density of color centers. Furthermore, images with different color filters are compared to provide the information of the composition of defect structures. Fluorescence lifetime image is performed for the emission dynamics of the nano-particle. The results indicate that the decay lifetime has an relation to the emission intensity. When the nano-particle contains more color centers, it quenches the emission from H3 centers more.

中文摘要 i
Abstract ii
目錄 iii
圖目錄 iv
第一章 緒論 1
第二章 鑽石簡介 3
2-1鑽石的性質 3
2-2鑽石缺陷所造成的發光性質 4
2-2.1 鑽石的分類 5
2-2.2 鑽石中氮所造成的缺陷 6
2-4螢光奈米鑽石的應用 11
第三章 實驗理論分析 13
3-1 螢光簡介 13
3-1-1 螢光的機制 13
3-1-2 螢光量子效率(fluorescence quantum yield) 18
3-1-3 螢光生命期(fluorescence lifetime) 18
第四章 單分子螢光量測方式與實驗架構 20
4-1 單分子螢光量測方式 20
4-2 利用光波干涉相的量測方式 22
4-3 實驗架構 24
4-4 實驗所用樣品 27
第五章 實驗步驟與結果 28
5-1 樣品準備 28
5-2 實驗結果與討論 29
5-2-1兩種尺寸gFND的螢光分布情形與散射光分布情形的比較 29
5-2-2 140nm粒徑 gFND的螢光光譜 31
5-2-1 140nm 粒徑gFND的螢光分布情形 33
5-2-4 140nm 與30nm 粒徑gFND的H3缺陷結構的螢光生命周期 36
第六章 結論 44
參考文獻 45

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