b-catenin蛋白扮演著細胞黏附結構分子與轉錄作用中共同活化因子之雙重角色，分別與E-cadherin蛋白鍵結形成黏附結構，或與TCF/LEF轉錄因子結合進行標的基因轉錄之活化。在正常情形下，細胞質中游離態的b-catenin會經由proteosome-dependent之機轉而降解，在惡性腫瘤細胞中，常因b-catenin失去調節，而造成b-catenin的異常堆積，進而形成癌症。本論文即在探討b-catenin在子宮頸癌細胞中形成堆積之原因及其與E-cadherin、p53蛋白，在不同FIGO (International Federation of Obstetrics and Gynecology)期別中表現情形之相關性。
藉由70例不同期別之侵入性子宮頸癌病理石臘包埋檢體，分別以b-catenin抗體及p53抗體進行免疫組織化學染色分析，並且針對其基因CTNNB1之exon 13進行直接定序分析。實驗結果顯示，有58例檢體(82.8%)之b-catenin表現於細胞質或細胞核，而未發現任何突變在CTNNB1 exon 13上，然而b-catenin的表現強度與轉移與否無關，但與腫瘤大小呈正相關(p=0.008)，且與E-cadherin表現強度成反向相關(p=0.027)，另外與p53之表現強度呈現顯著相關(p=0.013)，因此在子宮頸癌細胞中b-catenin的異常堆積，應與CTNNB1之exon 13是否突變無關，而異常的p53可能扮演著造成b-catenin異常堆積的重要因子。

b-catenin plays a dual role as a structural component of adherens junctions and as a transcriptional coactivator through its interactions with E-cadherin and TCF/LEF transcription factors, respectively. Normally, free b-catenin in cytoplasma is regulated by proteosome-dependent degradation system. In malignant tumor cell , deregulation of b-catenin degradation results in its aberrant accumulation, and leading to cancer.
The goals of this study were to explore the reason of aberrant b-catenin accumulation in cervical carcinoma and evaluate the correlation between b-catenin、E-cadherin and p53 in different FIGO stage.
Seventy paraffin embedded specimen with different FIGO stage were included in this study. Immunohistochemical staining was performed using anti-b-catenin polyclonal antibody and anti-p53 polyclonal antibody respectively and direct sequencing methods to analyze the mutation of CTNNB1 exon 13. The results showed 58 cases (82.8%) displayed cytoplasmic/nuclear b-catenin and no mutations in exon 13 of b-catenin gene, whereas no significant correlation between b-catenin expression level and tumor metastasis. However, b-catenin expression intensity had significant correlation with tumor size (p=0.008) and inversely correlated with E-cadherin (p=0.027) in different FIGO stage. The other way, the p53 staining intensity was significant correlated with b-catenin expression intensity (p=0.013) . Therefore, we suggest that mutations of CTNNB1 exon 13 may not be a reason for aberrant b-catenin accumulation in cervical carcinoma and aberrant p53 may play an important factor in accumulation of b-catenin.

中文摘要………………………………………………………………1
英文摘要………………………………………………………………2
背景介紹………………………………………………………………3
實驗目的………………………………………………………………8
材料與方法……………………………………………………………10
實驗結果………………………………………………………………19
討論……………………………………………………………………23
參考文獻………………………………………………………………27
圖表……………………………………………………………………31

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