斑鰭飛魚(Ｃypselurus poecilopterus)屬頜針魚目飛魚科，廣泛分佈於印度洋到西太平洋間，屬於大洋遷移性魚類，為台灣海域飛魚主要優勢種，亦為經濟魚種。本研究利用粒線體細胞色素b序列，分析斑鰭飛魚在台灣海域之族群遺傳結構，並根據前人文獻假設台灣海域斑鰭飛魚不只一個來源，而冬季則僅有一個來源。野外採樣於2010年12月到2012年1月，在宜蘭、花蓮、台東、屏東之定置漁網場，共採集85隻個體。以細胞色素b部分片段（1000 bp）進行分析，定序結果共有68個單倍基因型，有102個變異位，平均遺傳距離0.9%。親緣關係樹及TCS圖皆呈現兩個主要系群，系群一由夏季與冬季的單倍基因型組成，系群二僅由夏季的單倍基因型組成，也就是說夏季由兩個系群組成，冬季僅由一群，表示夏季有兩個來源，冬季則僅有一個來源。系群分佈與冬夏兩季洋流流向大致相關，兩個系群可能分別為隨著南海海流與南海暖流洄游之南海群及隨著黑潮洄游之黑潮群。在分子變方分析（AMOVA）中顯示，結果顯示變異分布在系群間（63%）顯著大於系群內的變異（37%），表示兩個系群間有顯著的遺傳分化。因此本研究結果支持台灣海域的斑鰭飛魚不只一個來源。

Ｃypselurus poecilopterus is a highly migratory fish and is widely distributed in the warm waters of the Indo-Pacific Ocean. It is a common flying fish of miniature size that resides in the waters near Taiwan. Previous studies suggested that C. poecilopterus around Taiwan come from more than one source, and the winter population should be from single source. In this study, I examined the genetic structure ofＣ. poecilopterus populations in the waters off Taiwan using mitochondrial cytochrome b sequences. A total of 85 individuals were collected from set nets in Pingtung, Taitung, Hualien, and Ilan. In total, I found 68 haplotypes of cytochrome b sequences, of which the nucleotide length was 1,000 bp, including 102 informative sites. The mean genetic distance was 0.9%. Two major clades were revealed in the phylogenetic trees and the TCS haplotype network. Clade 1 includes the summer and winter haplotypes, Clade 2 includes only the summer haplotypes. The relative positions of the haplotypes in the clades corresponded to the current flow directions of summer and winter. The haplotypes of Clade 1 may come from the South China Sea and migrate with the South China Sea surface current and South China Sea warm current. The haplotypes of Clade 2 may come from the waters of the East Philippines and migrate with the Kouroshio. Based on the AMOVA results, the variation among clades (63.7%) was significantly greater than the variations within clades (36.3%), suggesting that the two populations have significant genetic differentiation. Therefore, the results support that C. poecilopterus around Taiwan come from more than one source.

Contents

論文審書……………………i
誌謝……………………………ii
摘要……………………………iii
Abstract…………………………iv
Contents…………………………v
Figure Legend……………………vi
Table Legend……………………vii
Introduction………………………1
Materials and Methods…………6
Results………………………………10
Discussion…………………………13
Literature Cited……………………18
Figures…………………………………27
Tables…………………………………33

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