中文摘要
耐輻射奇異球菌 (Deinococcus radiodurans) 對離子輻射及紫外線具有高度的耐受性 , 且對錳離子有很強的攝取性。於生長靜止期初期時加入二價錳離子會誘導細胞的再次分裂 , 產生二次生長的情形 (Mn-CD效應)。雖然此菌對糖類的利用效率不佳 , 然而確實可以代謝葡萄糖和果糖。我們發現耐輻射奇異球菌對於糖類的分解具有糖解路徑 (glycolysis) 及pentose phosphate pathway (PPP) 代謝路徑 , 且會受到錳離子的影響而改變其代謝路徑的走向和比例。在糖解路徑中我們以aldolase酵素活性做指標 , 而在PPP路徑中則以transketolase酵素活性作為指標, 結果顯示錳離子有促進glycolysis分解葡萄糖的效應 , 但是在不同的培養基中其酵素活性結果不一定相似。而PPP在各種培養基中生長時, 亦會有不一樣的活性表現。

ABSTRACT


Deinococcus radiodurans is extremely resistant to ionizing and UV irradiation. The addition of manganese(Ⅱ) into stationary-phase culture can trigger a new cycle of cell division ( Mn-CD effect ). Although D.radiodurans can hardly utilize sugar , but it does can incorporate glucose and fructose into the cell mass. We found that D. radiodurans can metabolize sugar by glycolysis ( EMP ) and pantose phosphate pathway ( PPP ). The activity of both pathways would be altered by the addition of manganese(Ⅱ). The marker enzymes of the PPP ( transketolase ) and EMP pathway ( aldolase ) were also analyzed. Results show that manganese(Ⅱ) can induce the hydrolysis of glucose by activating the EMP pathway. However , the induction effect various with different cultural conditions. In analyzing the PPP pathway , the activities of transketolase were also various with different cultural conditions.

目錄

中文摘要 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

英文摘要 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

壹、 前言. . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

貳、 材料與方法. . . . . . . . . . . . . . . . . . . . . . . . 17

參、 結果. . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

肆、 討論. . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

伍、 參考資料. . . . . . . . . . . . . . . . . . . . . . . . . .32

陸、 圖表. . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

伍、參考資料

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