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博碩士論文 etd-0223104-191503 詳細資訊
Title page for etd-0223104-191503
論文名稱
Title
台灣西海岸蚵岩螺族群Ark (arginine kinase)同功酵素之時空變化
Temporal and Spatial Variations of Arginine Kinase Allozyme Among Populations of Thais clavigera From the West Coasts of Taiwan
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
46
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2004-01-30
繳交日期
Date of Submission
2004-02-23
關鍵字
Keywords
蚵岩螺、族群遺傳、時空變化、同功酵素
temporal and spatial variation, arginine kinase, Thais clavigera, population genetics
統計
Statistics
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The thesis/dissertation has been browsed 5696 times, has been downloaded 2386 times.
中文摘要
前蚵岩螺族群遺傳研究顯示arginine kinase (Ark)基因型頻率在香山、台西、布袋、七股等四地點有分化的現象。本研究探討地點間Ark基因頻率的差異是否源自各地點有不同之補充群,以蛋白質電泳技術來檢測蚵岩螺個體的Ark基因型,探討蚵岩螺Ark基因型頻率在空間上(香山、台西、布袋、七股)及時間上(體型:<15mm, 15-20mm, 20-30mm, 30-40mm, >40mm及年間:2001, 2003)的變化。結果顯示補充進入各地點的補充群(<15mm)Ark基因型頻率(ark63/63, ark63/100, ark100/100),除布袋外皆無顯著差異,而在體型15-20mm類群ark63/63頻率有由南自北漸增的現象,分別為七股(0.21)<布袋(0.33)<台西(0.60)<香山(0.66),推測可能與對高溫或乾燥之耐受力有關;而雌雄間Ark基因型頻率差異最顯著在台西30-40mm類群,但Ark基因型頻率地點間差異則與雌螺雄化程度無關。
Abstract
The variation of Arginine kinase (Ark) genotype frequency among populations of Thais clavigera has been reported in previous study. However, the difference which is originated from self-seeding or differential selection after recruitment is not understood. To answer this question, the spatial (i.e. Shainsan, Taisi, Budai, and Chiku) and temporal (sell length: <15mm, 15-20mm, 20-30mm, 30-40mm, and >40mm; year: 2001 and 2003) variations of Ark allozyme were examined by starch gel electrophoresis. There was no significant difference in the ratio of Ark genotypes (ark63/63, ark63/100, ark100/100) in the size range of <15mm among Shainsan, Taisi and Chiku. The result indicated that the populations of T. clavigera is from the same recruitment. In the size range of 15-20mm, the frequency of ark63/63 increased from southern to northern populations, i.e. 0.21(Chiku) < 0.33(Budai) < 0.60(Taisi) < 0.66(Shainsan), respectively. It is proposed that the variation of Ark genotypes among populations is resulting from stress of temperature or dessication. A significant difference in the ratio of Ark genotypes was found in the population of Taisi with the size range of 30-40mm. Besides, the frequency of Ark genotypes among populations was not related to the degree of imposex.
目次 Table of Contents
目錄章次 頁次
中文摘要……………………………………………………………Ⅲ
英文摘要……………………………………………………………Ⅳ
目錄…………………………………………………………………Ⅴ
表目錄………………………………………………………………Ⅵ
圖目錄………………………………………………………………Ⅶ
一、緒論……………………………………………………………1
二、材料與方法……………………………………………………5
三、結果……………………………………………………………8
四、討論……………………………………………………………14
參考文獻……………………………………………………………21
個人履歷……………………………………………………………46
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