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博碩士論文 etd-0223106-154023 詳細資訊
Title page for etd-0223106-154023
論文名稱
Title
蚵岩螺Arginine Kinase同功異構酵素基因型頻率與潮位之相關性
The study og arginine kinase allozyme of Thais clavigera in relation to tides
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
62
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2006-01-23
繳交日期
Date of Submission
2006-02-23
關鍵字
Keywords
蚵岩螺、Ark基因型頻率、潮位
Thais clavigera, arginine kinase, tide
統計
Statistics
本論文已被瀏覽 5683 次,被下載 1949
The thesis/dissertation has been browsed 5683 times, has been downloaded 1949 times.
中文摘要
由過去的族群遺傳研究得知,蚵岩&#63761;在臺灣西海岸為同一族群,但是其arginine kinase (Ark) 基因型頻&#63841;在各地點有&#63847;同的現象,這種基因型頻&#63841;的差&#63842;主要發生在較大體型&#63761;上。雖然造成Ark 基因型有差&#63842;的因子有很多,溫&#64001;和乾燥是其中重要的二個影響因子,而潮位差&#63842;和此二因子有直接相關,因此,本研究以蚵岩&#63761;Ark 基因型頻&#63841;是否因潮位&#63847;同而有差&#63842;為題進&#64008;實驗,實驗分三部分進&#64008;:一、探討蚵岩&#63761;Ark 基因型頻&#63841;隨體型與潮位的變化情形;二、探討&#63847;同Ark 基因型的蚵岩&#63761;對於室內環境乾燥時間&#63847;同的選擇性;三、&#63847;同Ark 基因型酵素活性之檢測。實驗一發現台西蚵岩&#63761;的Ark 基因型頻&#63841;分佈比&#63925;在8 月份體型25-35mm 的個體在&#63847;同潮位有差&#63842;,高潮位Ark63/100 較多(0.47∼0.56),低潮位則是Ark63/63 較多(0.49∼0.66)。整體而言,小體型(<25mm)
的Ark63/63 基因型的比&#63925;較高,大體型(>35mm)則是Ark63/100 較多,顯示隨著體型的改變Ark 基因型頻&#63841;有差&#63842;。實驗二則顯示蚵岩&#63761;&#63847;同Ark 基因型對乾燥時間&#63847;同的水位環境有選擇性,室內高水位區以Ark63/100 佔多&#63849;(0.48),室內低水位區則是Ark63/63 較多(0.54),此結果和野外台西8 月體型為25-35mm 個
體的分佈&#63994;況&#63952;似:野外高潮位與室內高水位&#64038;是Ark63/100 較多,而野外低潮位與室內低水位則是Ark63/63 較多。實驗三的結果顯示三種Ark 基因型的酵素活性沒有顯著差&#63842;,&#63847;過三種溫&#64001;(18℃、24℃、30℃)下Ark63/63 的活性趨勢&#64038;&#63862;高於Ark63/100 與Ark100/100。而蚵岩&#63761;Ark 基因型頻&#63841;隨著潮位與體型的&#63847;同而改變,可能是受環境乾燥時間、溫&#64001;以及生殖耗能等因素影響所造成。
Abstract
Previous studies in the population genetics of the oyster drill Thais clavigera show that snails from the west coast of Taiwan belong to the same population. However, the
genotype frequency of arginine kinase (Ark) in large snails is different among sites in the west coast. In general, changes of temperature and desiccation caused by tidal cycle are the most important twp environmental factors affect genetic variation of intertidal organisms. In the present study, the relationship between the frequency of Ark genotype and tide was evaluated through the following experiments. It has been found that the frequencies of Ark genotype of snails, collected in August with the size range of 25-35mm, were correlated to tides. A higher grequency of Ark63/100 (0.47~0.56) was in the upper intertidal and a higher frequency of Ark63/63 (0.49~0.66) was at the lower end. Moreover, the genotype frequency also varied with size, i.e. more Ark63/63 in
small snails (<25mm) and more Ark63/100 in large snails (>35mm). Laboratory desiccative experiments indicated that snails with genotype of Ark63/100 preferred in the areas of high water level and Ark63/63 in areas of low water level. The laboratory results were consistent with the field data. To compare the enzyme activity, there was no
difference among the genotypes of Ark63/63, Ark63/100 and Ark100/100at temperatures of 18, 24 and 30℃. In summary, the frequencies of Ark genotype varied with tide and size
and factors such as temperature, desiccation and reproduction may play important roles in the relationship.
目次 Table of Contents
謝辭…………………………………………………… Ⅰ
中文摘要……………………………………………… Ⅱ
英文摘要……………………………………………… Ⅲ
目&#63807;…………………………………………………… Ⅳ
表目&#63807;………………………………………………… Ⅴ
圖目&#63807;………………………………………………… Ⅵ
附表目&#63807;……………………………………………… Ⅶ
附圖目&#63807;……………………………………………… Ⅷ
一、緒&#63809;……………………………………………… 1
二、材&#63934;與方法……………………………………… 6
三、結果……………………………………………… 10
四、討&#63809;……………………………………………… 16
&#63851;考文獻……………………………………………… 22
個人&#63967;&#63884;……………………………………………… 54
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