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論文名稱 Title |
蚵岩螺Arginine Kinase同功異構酵素基因型頻率與潮位之相關性 The study og arginine kinase allozyme of Thais clavigera in relation to tides |
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系所名稱 Department |
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畢業學年期 Year, semester |
語文別 Language |
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學位類別 Degree |
頁數 Number of pages |
62 |
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研究生 Author |
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指導教授 Advisor |
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召集委員 Convenor |
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口試委員 Advisory Committee |
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口試日期 Date of Exam |
2006-01-23 |
繳交日期 Date of Submission |
2006-02-23 |
關鍵字 Keywords |
蚵岩螺、Ark基因型頻率、潮位 Thais clavigera, arginine kinase, tide |
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統計 Statistics |
本論文已被瀏覽 5683 次,被下載 1949 次 The thesis/dissertation has been browsed 5683 times, has been downloaded 1949 times. |
中文摘要 |
由過去的族群遺傳研究得知,蚵岩螺在臺灣西海岸為同一族群,但是其arginine kinase (Ark) 基因型頻率在各地點有不同的現象,這種基因型頻率的差異主要發生在較大體型螺上。雖然造成Ark 基因型有差異的因子有很多,溫度和乾燥是其中重要的二個影響因子,而潮位差異和此二因子有直接相關,因此,本研究以蚵岩螺Ark 基因型頻率是否因潮位不同而有差異為題進行實驗,實驗分三部分進行:一、探討蚵岩螺Ark 基因型頻率隨體型與潮位的變化情形;二、探討不同Ark 基因型的蚵岩螺對於室內環境乾燥時間不同的選擇性;三、不同Ark 基因型酵素活性之檢測。實驗一發現台西蚵岩螺的Ark 基因型頻率分佈比例在8 月份體型25-35mm 的個體在不同潮位有差異,高潮位Ark63/100 較多(0.47∼0.56),低潮位則是Ark63/63 較多(0.49∼0.66)。整體而言,小體型(<25mm) 的Ark63/63 基因型的比例較高,大體型(>35mm)則是Ark63/100 較多,顯示隨著體型的改變Ark 基因型頻率有差異。實驗二則顯示蚵岩螺不同Ark 基因型對乾燥時間不同的水位環境有選擇性,室內高水位區以Ark63/100 佔多數(0.48),室內低水位區則是Ark63/63 較多(0.54),此結果和野外台西8 月體型為25-35mm 個 體的分佈狀況類似:野外高潮位與室內高水位都是Ark63/100 較多,而野外低潮位與室內低水位則是Ark63/63 較多。實驗三的結果顯示三種Ark 基因型的酵素活性沒有顯著差異,不過三種溫度(18℃、24℃、30℃)下Ark63/63 的活性趨勢都略高於Ark63/100 與Ark100/100。而蚵岩螺Ark 基因型頻率隨著潮位與體型的不同而改變,可能是受環境乾燥時間、溫度以及生殖耗能等因素影響所造成。 |
Abstract |
Previous studies in the population genetics of the oyster drill Thais clavigera show that snails from the west coast of Taiwan belong to the same population. However, the genotype frequency of arginine kinase (Ark) in large snails is different among sites in the west coast. In general, changes of temperature and desiccation caused by tidal cycle are the most important twp environmental factors affect genetic variation of intertidal organisms. In the present study, the relationship between the frequency of Ark genotype and tide was evaluated through the following experiments. It has been found that the frequencies of Ark genotype of snails, collected in August with the size range of 25-35mm, were correlated to tides. A higher grequency of Ark63/100 (0.47~0.56) was in the upper intertidal and a higher frequency of Ark63/63 (0.49~0.66) was at the lower end. Moreover, the genotype frequency also varied with size, i.e. more Ark63/63 in small snails (<25mm) and more Ark63/100 in large snails (>35mm). Laboratory desiccative experiments indicated that snails with genotype of Ark63/100 preferred in the areas of high water level and Ark63/63 in areas of low water level. The laboratory results were consistent with the field data. To compare the enzyme activity, there was no difference among the genotypes of Ark63/63, Ark63/100 and Ark100/100at temperatures of 18, 24 and 30℃. In summary, the frequencies of Ark genotype varied with tide and size and factors such as temperature, desiccation and reproduction may play important roles in the relationship. |
目次 Table of Contents |
謝辭…………………………………………………… Ⅰ 中文摘要……………………………………………… Ⅱ 英文摘要……………………………………………… Ⅲ 目錄…………………………………………………… Ⅳ 表目錄………………………………………………… Ⅴ 圖目錄………………………………………………… Ⅵ 附表目錄……………………………………………… Ⅶ 附圖目錄……………………………………………… Ⅷ 一、緒論……………………………………………… 1 二、材料與方法……………………………………… 6 三、結果……………………………………………… 10 四、討論……………………………………………… 16 參考文獻……………………………………………… 22 個人履歷……………………………………………… 54 |
參考文獻 References |
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