摘 要

耐輻射奇異球菌具有高度的抗輻射性，是株革蘭氏陽性、絕對好氧的四聯球菌，其營養方式是屬於化學異營性。在細胞生長靜止期初期加入2.5 μM的二價錳離子，可誘導細胞再次生長分裂(Mn-CD效應)，為此菌特有的現象。本實驗利用complex medium培養，並添加各種醣類作為碳源，來探討本菌對於各種單醣與雙醣所產生的反應，發現無論是否添加錳離子，果糖的比生長速率均大於葡萄糖的比生長速率；但培養基中含錳離子濃度過高時，則會抑制此菌生長，隨錳離子濃度增高，lag phase亦隨之延長。另外我們利用chemically defined medium培養此菌，發現碳源為glucose、fructose、mannose、maltose、sucrose時生長情況較為良好，且均有Mn-CD效應的發生。而galactose、lactose、mannitol，則生長情況差，亦無Mn-CD效應。











I

Abstract

The highly radioresistant Deinococcus radiodurans is a heterotrophic Gram positive aerobic tetrad. The addition of 2.5 uM Mn(II) into the early stationary-phase culture would trigger a new round of cell division (Mn-CD effect) which is specific to this unique bacterium. In order to understand the reactions of this bacterium to different carbohydrates, we culture the bacterium by using complex media with various monosaccharides and disaccharides.
No matter in the present of Mn(II) or not, the specific growth rate of fructose–grown cells was always higher than those of glucose-grown cells. Cell growth would be inhabited by excess Mn(II). Along with the increasing of Mn(II), the culture showed a prolonged lag phase. In chemically defined media, this bacterium grew well by using glucose, fructose, mannose, maltose or sucrose as it’s sole carbon source and performed the Mn-CD growth by adding Mn(II).








II

目 錄

中文摘要……………………………………………….I
英文摘要………………………………………………II
壹、前言……………………………………………….1
貳、材料與方法………………………………………13
參、結果………………………………………………19
肆、討論………………………………………………25
伍、參考文獻…………………………………………32
陸、圖表………………………………………………39

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