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博碩士論文 etd-0408117-194004 詳細資訊
Title page for etd-0408117-194004
論文名稱
Title
以氮化鋁鎵/氮化鎵高電子遷移率場效電晶體生物感測器偵測胰臟癌CEA及CA19-9抗原分子
Detection of pancreas cancer markers CEA and CA19-9 antigen by AlGaN/GaN high electron mobility transistor biosensor
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
65
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2017-01-11
繳交日期
Date of Submission
2017-05-08
關鍵字
Keywords
CA19-9、生物感測器、氮化鋁鎵/氮化鎵高電子遷移率場效電晶體、CEA
CA19-9, biosensor, CEA, AlGaN/GaN HEMT
統計
Statistics
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中文摘要
氮化鋁鎵/氮化鎵高電子遷移率場效電晶體具有電子遷移高、靈敏度高、高效率等優點,因此這類材料適合用來偵測帶電量極少生物分子,可以利用這種材料發展生物感測晶片,達到便利、減少檢體、多重檢測等優點。本研究希望將氮化鋁鎵/氮化鎵高電子遷移率場效電晶體應用於偵測生物分子,做出快速篩檢、小尺寸、便利的生物感測器。
氮化鋁鎵/氮化鎵高電子遷移率場效電晶體是由三五族氮化物組成,其中氮化鋁鎵與氮化鎵的異質接面處會形成二維電子氣結構。當生物分子修飾在電晶體表面上時,會改變二維電子氣體的載子分布進而影響載子傳導特性。我們利用LabVIEW軟體量測即時電流曲線圖,判斷生醫晶片的電導變化。
本研究偵測CEA抗原分子及CA19-9抗原分子,用以增加生物感測晶片的可信度。利用兩個感測區分別修飾不同抗體,當帶有兩種抗原的液體流經感測區時,及可分別偵測。
Abstract
AlGaN / GaN high electron mobility transistors (HEMTs) have the advantages of high electron mobility, high sensitivity, and high efficiency. Therefore, HEMTs are suitable for the detection of biomolecules with very little charge and can utilize HEMTs to develop biosensor. The aim of this study is to investigate the application of AlGaN / GaN HEMT in the detection of biomolecules, and to make rapid screening, small size and convenient biosensor.
AlGaN/GaN HEMT hetero structure interface form a two-dimensional electron gas (2DEG). The bio-molecules bind on the HEMT surface will change the electron concentration of 2DEG. When biomolecules bind with the AlGaN/GaN HEMT, the electron concentration in the 2DEG structure will change. We use LabVIEW software to measure the immediate current and the conductance change.
In this study, CEA and CA19-9 antigen cancer tumor markers are detected to increase the reliability of the biosensor. Two sensing areas are respectively modified with different antibodies. When the liquid within two antigens flows through the sensing areas, and can be detected separately.
目次 Table of Contents
論文審定書 i
摘要 ii
Abstract iii
圖目錄 vi
第一章 序論 1
1.1 生物感測器介紹 1
1.2 AlGaN/GaN高電子遷移率電晶體 (High Electron Mobility Transistor, HEMT) 2
1.3 腫瘤標誌物 4
1.4 生物晶片量測原理 6
第二章 儀器介紹及架設 7
2.1 光學微影系統 (Mask Aligner and Exposure System) 7
2.2 雙電子束蒸鍍系統 (Dual E-beam Evaporator, E-beam Evaporator) 8
2.3 快速退火系統 (Rapid Thermal Annealing System, RTA System) 9
2.4 高密度電漿化學氣相沈積系統 (High Density Plasma Chemical Vapor Deposition, HDP CVD) 10
2.5 感應耦合式電漿蝕刻系統 (Inductive Couple Plasma Etcher, ICP Etcher) 11
2.6 電性量測系統 12
2.7 即時偵測系統 (Lock-in Amplifier) 13
2.8 掃描式電子顯微鏡 (Scanning Electron Microscopy, SEM) 16
2.9 X射線繞射分析儀 (X-Ray Diffraction, XRD) 17
第三章 製程流程 18
3.1 生物晶片製程 18
3.2 微流體通道製程 23
3.3 生物分子表面修飾 25
第四章 實驗結果與討論 28
4.1 HEMT基板結構分析結果 28
4.2 快速熱退火結果 31
4.3 生物晶片製程閘極控制結果 32
4.4 感測區表面修飾結果 40
4.5 流體背景測試結果 43
4.6 流體實驗結果 45
第五章 結論與未來展望 48
附錄一 電性量測系統介紹 49
附錄二 即時量測系統介紹 50
附錄三 樣品表 53
Reference 54
參考文獻 References
1. Gold P, Freedman SO. (1965). Specific carcinoembryonic antigens of the human digestive system. J Exp Med, 121, 439.
2. Tran, T. T., & Mulchandani, A. (2016). Carbon nanotubes and graphene nano field-effect transistor-based biosensors. TrAC - Trends in Analytical Chemistry, 79, 222–232.
3. Ping, Jinglei et al. (2015). “Quantifying the Effect of Ionic Screening with Protein-Decorated Graphene Transistors.” Biosensors and Bioelectronics 89: 689– 92.
4. Li, Chao et al. (2005). “Complementary Detection of Prostate-Specific Antigen Using In2O3 Nanowires and Carbon Nanotubes.” Journal of the American Chemical Society 127(36): 12484–85.
5. Yang, Ning et al. (2015). “Carbon Nanotube Based Biosensors.” Sensors and Actuators, B: Chemical 207(PartA): 690–715.
6. Lee, Min-Ho et al. (2010). “Measurements of Serum C-Reactive Protein Levels in Patients with Gastric Cancer and Quantification Using Silicon Nanowire Arrays.” Nanomedicine, nanotechnology, biology, and medicine 6(1): 78–83.
7. Presnova, Galina et al. (2016). “Biosensor Based on a Silicon Nanowire Field-Effect Transistor Functionalized by Gold Nanoparticles for the Highly Sensitive Determination of Prostate Specific Antigen.” Biosensors and Bioelectronics 88: 1– 7.
8. Kwon, Insu et al. (2011). “Extended-Gate Metal Oxide Semiconductor Field Effect Transistor-Based Biosensor for Detection of Deoxynivalenol.” Japanese Journal of Applied Physics 50: 06GL08.
9. Oh, Yunkwang et al. (2016). “Journal of Industrial and Engineering Chemistry Metal Oxide Semiconductor Field-Effect Transistor ( MOSFET ) -Based Direct Monitoring of p53 in Spiked Serum.” Journal of Industrial and Engineering Chemistry 37: 95–100.
10. Guo, Zhibo, Lai Wang, Zhibiao Hao, and Yi Luo. (2013). “Modeling and Experimental Study on Sensing Response of an AlGaN/GaN HEMT-Based Hydrogen Sensor.” Sensors and Actuators, B: Chemical 176: 241–47.
11. Myers, M., Khir, F. L. M., Podolska, A., Umana-Membreno, G. A., Nener, B., Baker, M., & Parish, G. (2013). Nitrate ion detection using AlGaN/GaN heterostructure-based devices without a reference electrode. Sensors and Actuators, B: Chemical, 181, 301–305.
12. Brazzini, T., A. Bengoechea-Encabo, M. A. Sánchez-García, and F. Calle. (2013). “Investigation of AlInN Barrier ISFET Structures with GaN Capping for pH Detection.” Sensors and Actuators, B: Chemical 176: 704–7.
13. Huang, C.-C., Lee, G.-Y., Chyi, J.-I., Cheng, H.-T., Hsu, C.-P., Hsu, Y.-R., … Wang, Y.-L. (2013). AlGaN/GaN high electron mobility transistors for protein-peptide binding affinity study. Biosensors & Bioelectronics, 41, 717–22.
14. Espinosa, Nayeli, Stefan U. Schwarz, Volker Cimalla, and Oliver Ambacher. (2015). “Detection of Different Target-DNA Concentrations with Highly Sensitive AlGaN/GaN High Electron Mobility Transistors.” Sensors and Actuators, B: Chemical 210: 633–39. http://dx.doi.org/10.1016/j.snb.2015.01.019.
15. Kamisawa, T., Wood, L. D., Itoi, T., & Takaori, K. (2016). Pancreatic cancer. Lancet (London, England), 388(10039), 73–85.
16. L. Clark Jr., & C. Lyons. (1962). Electrode systems for continuous monitoring in cardiovascular surgery. Ann. NY Acad. Sci., 102, 29-45.
17. Lee, K. J., Yi, S. W., Chung, M. J., Park, S. W., Song, S. Y., Chung, J. B., & Park, J. Y. (2013). Serum CA 19-9 and CEA levels as a prognostic factor in pancreatic adenocarcinoma. Yonsei Medical Journal, 54(3), 643–649.
18. Wu, L., Huang, P., Wang, F., Li, D., Xie, E., Zhang, Y., & Pan, S. (2015). Relationship between serum CA19-9 and CEA levels and prognosis of pancreatic cancer. Annals of Translational Medicine, 3(21), 328.
19. Goonetilleke, K. S., & Siriwardena, A. K. (2007). Systematic review of carbohydrate antigen (CA 19-9) as a biochemical marker in the diagnosis of pancreatic cancer. European Journal of Surgical Oncology, 33(3), 266–270.
20. Mcpherson, Richard A, and Matthew R Pincus. (2011). 14 Henry ’ S Clinical Diagnosis and Management by Laboratory Methods.
21. Bergquist, J. R., Puig, C. A., Shubert, C. R., Groeschl, R. T., Habermann, E. B., Kendrick, M. L., … Truty, M. J. (2016). Carbohydrate antigen 19-9 elevation in anatomically resectable, early stage pancreatic cancer is independently associated with decreased overall survival and an indication for neoadjuvant therapy: A national cancer database study. Journal of the American College of Surgeons, 223(1), 52–65.
22. T. Malati. (2007). Tumor markers: An overview. Ind. J. Clinical Biochemistry. 22, Issue 2, 17-31.
23. Pavai, P., & Yap, S. (2003). The clinical significance of elevated levels of serum CA. Medical Journal of Malaysia., 58(5), 667–72.
24. Ballehaninna, U. K., & Chamberlain, R. S. (2012). The clinical utility of serum CA 19-9 in the diagnosis, prognosis and management of pancreatic adenocarcinoma: An evidence based appraisal. Journal of Gastrointestinal Oncology, 3(2), 105–119.
25. Kozlowski LP (2016) IPC - Isoelectric Point Calculator. Biology Direct 11:55.
26. 陳力輔, 氮化鎵異質結構場效電晶體之研究, (2005).
27. Baur, B., Steinhoff, G., Hernando, J., Purrucker, O., Tanaka, M., Nickel, B., & Eickhoff, M. (2005). Chemical functionalization of GaN and AlN surfaces. Applied Physics Letters, 87(26), 1–3.
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