最近由於梅雨季節所帶來的暴雨而造成水處理廠水源受到大腸桿菌及有機物污染，這些水質項目之濃度均高於飲用水水源水質標準，故會影響處理水質。為了降低水處理廠加氯消毒所衍生之消毒副產物(Disinfection By-Products, DBPs)，有必要於前處理去除原水中大腸桿菌及有機物。
本研究運用臭氧反應槽串聯及生物活性碳管柱，以二種系統(以T1、T2表示)之現場模組，探討去除原水中大腸桿菌及有機物之效能。使用培養在地菌種及使用該地區之原水馴養生物活性碳以提高生物降解有機物的效率。本研究所使用的原水取自高屏溪河川並實驗中以生物可利用有機碳(AOC) 代表生物降解，AOC中P17此菌種之產率為2.41×106 CFU /μg acetate-C，NOx此菌種之產率為7.82×106 CFU / μg acetate-C。
結果顯示，臭氧對大腸桿菌群及總菌落數在5分鐘以內之去除率已高達99％，表示臭氧對大腸桿菌群及總菌落數為一有效的前處理方法。
TOC、DOC、AOC等原水中有機物，去除率約40至80％。生物活性碳(BAC)的關鍵去除有機物操作條件為停留時間及臭氧濃度。當原水以足夠臭氧量串聯BAC時，因有機物之去除率可比原水直接進入BAC高出10至20％。因此，前臭氧處理可以有效提升BAC對有機物生物降解效能。

Because of the heavy rain in plum rain season recently, the water source from water treatment factory was polluted by E coli and organic. The concentration of these water quality items is higher than the standard of drinking water source quality. So, it cannot be supplied to public. In order to reduce the DBPs that caused of interaction between the remaining chlorine and organic when disinfection of water treatment plant is adding chlorine, it is very important to eliminate E coli and organic using pretreatment before the water treatment plant.
We use the module of 2 processes of will ozone reactor and biological activated carbon column, the research mainly will discuss the elimination for E coli and organic efficiency from raw water. We cultivate local bacteria from the raw in biological activated carbon to increase the efficiency of AOC. For the results of this experiment, it regards creature usable carbon as AOC. For AOC, the productive yield of bacteria P17 is 2.41x106 CFU /μg acetate-C, while the productivity of bacteria NOX is 7.82×106 CFU /μg acetate-C. The above AOC values in raw water was sampling from Kao-Ping River. Therefore, the yields can be treated as AOC of Kao-Ping River.
The results indicated that elimination rate of E coli reach to 99% in five minutes for the influence of ozone to E coli and total colony number. It indicates ozone is the good pre-treatment method for removal of E coli and total colony.
TOC, DOC and AOC are three indicators of stands for the organic in the raw water. The elimination rate of these organic is ranging from 40 to 80%. The key operation conditions of BAC are the empty bed contact time and the concentration of ozone. We found the removal efficiency is more 10 to 20% in the way of ozone combined BAC, than the way of only BAC for treating raw water. It can be seen the ozone pre-treatment can increase the removal efficiency of AOC in followed BAC.

摘要 i
ABSTRACT .iii
目錄 v
圖目錄 x
表目錄 xii
第一章 前言 1
1-1 研究緣起 1
1-2 研究目的 2
1-3研究內容 3
第二章 文獻回顧 4
2-1 水中有機物的種類與來源 4
2-1-1水中背景有機物之性質 5
2-1-2水中背景有機物之結構分析 7
2-1-3　水中生物可分解有機質之測定 9
2-2 臭氧氧化之原理及程序 11
2-2-1臭氧物理化學性質 11
2-2-2 臭氧的自解機制 12
2-2-3 PH 值對臭氧反應特性之影響 14
2-2-4臭氧對於生物分解性的影響 15
2-2-5臭氧在給水場之應用 16
2-3 活性碳 20
2-4 高屏溪攔河堰概況 22
第三章 研究方法 24
3-1 研究流程之規畫 24
3-2 臭氧處理程序實驗法 25
3-3 生物活性碳(BAC)程序實驗方法 .26
3-4 水質分析項目與方法 27
3-4-1水溫與氫離子濃度指數 29
3-4-2導電度 29
3-4-3總溶解固體物 29
3-4-4濁度 29
3-4-5總有機碳 30
3-4-6溶解性有機碳 30
3-4-7生物可利用有機碳 30
3-4-7-1 純菌菌液的預先培養 30
3-4-7-2 菌種活化、培養與保存 34
3-4-9-3 菌種純種鑑定與特性分析 37
3-4-7-4 AOC器皿之清洗方式 40
3-4-7-5 P17與NOX生長曲線與產率之求得 41
3-4-7-6 水樣AOC之分析方式 43
3-4-8氨氮 44
3-4-9化學需氧量 44
3-4-10大腸桿菌群 44
3-4-11總菌落數 45
3-4-12臭氧濃度 45
3-5實驗分析儀器設備 46
第四章 結果與討論 48
4-1AOC之菌種(P17及NOX)生長曲線及產率值(YIELD) 48
4-2生物活性碳(BAC)預前的微生物馴養 55
4-2-1 TOC及DOC濃度之變化 55
4-3臭氧對原水水質之處理效率 59
4-3-1臭氧濃度及溶解度測試 59
4-3-2臭氧對原水水質之處理效能 62
4-3-2-1 COD之去除率 62
4-3-2-2臭氧對原水大腸桿菌群及總菌落數之處理效能 65
4-4現場模組對經臭氧與未經臭氧串聯生物活性碳對TOC及DOC處理效能 68
4-4-1總有機碳(TOC)去除率 70
4-4-2-1比較，T1與T2在對DOC去除率 70
4-4-2溶解性有機碳(DOC) 73
4-4-2-1比較，T1與T2在對DOC去除率 73
4-5現場模組對經臭氧與未經臭氧串聯生物活性碳對AOC處理效能 76
4-5-1綜合T1與T2對AOC去除率之比較 76
第五章結論與建議 80
5-1結論 80
5-2建議 .81
參考文獻 82

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