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博碩士論文 etd-0601111-234235 詳細資訊
Title page for etd-0601111-234235
論文名稱
Title
四種柵藻(Desmodesmus)之篩選與定性:光合作用、耐熱性及油脂生產
Isolation and characterization of four Desmodesmus green microalgae: Photosynthesis, Heat tolerance and Oil production
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
37
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2011-06-01
繳交日期
Date of Submission
2011-06-01
關鍵字
Keywords
生質柴油、微藻、耐熱性、缺氮、光系統效率
Biodiesel, Microalgae, Thermo-tolerance, Nitrogen starvation, Photosystem efficiency
統計
Statistics
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中文摘要
微細藻類在作為生產生質柴油的原料上具有很大的潛力。熱帶及亞熱帶地區比溫帶地區有更多的太陽能可以用來培養微藻,然而戶外強光導致培養液的溫度升高,對目前大多數已知的微藻是有害的。就建立產業而言,理想中的微藻應具備有耐熱性、高生長率和高油脂含量的特性。台灣南部位於亞熱帶地區,本研究篩選並分離出四株新的綠藻,這四株藻類是屬於Senedesmaceae科中Desmodesmus屬的成員。在這四株藻中有兩株在45℃小時的熱處理下, 高溫所造成的死亡率只在5到13%之間,缺氮處理下有兩株的油脂含量超過乾重之50%。另外,在缺氮處理之下四個物種的油脂累積和光系統效率呈現正相關(R2=0.90)。
Abstract
Microalgae hold a great potential to serve as feedstocks for biodiesel production. Tropical and subtropical zones have more solar energy than temperate areas for microalgal culturing. However, outdoor high irradiance results in high temperature in the culturing medium, which is damaging if not lethal to most known microalgal species. New microalgae with heat tolerance, high growth rates and high lipid contents are desirable to establish this industry. Four new green microalgae were isolated in southern Taiwan, located in the subtropical zone. All four species are members of the genus Desmodesmus in the family Senedesmaceae. Two of the four species survived at 45℃for 24 hours, with 5 to 13% mortality rates caused by the heat. Lipid contents of two species reached over 50% in dry biomass under nitrogen starvation. Oil accumulation in tion. Oil accumulation in tion. Oil accumulation in tion. Oil accumulation in the four species positively correlates with their photosystem efficiencies during stress treatments (R2=0.90).
目次 Table of Contents
Preface i
Acknowledgements i
摘要 ii
Abstract iii
Contents iv
List of Figures v
List of Tables vi
1. Introduction 1
2. Materials and Methods 3
3. Results 9
4. Discussion 13
5.Conclusions 16
6. References 17
Figures 20
Tables 26
Appendix 29
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