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論文名稱 Title |
肺癌及食道癌檢體中BRAF致癌基因突變之分析 Analysis of BRAF gene mutation in lung cancer and esophageal cancer |
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系所名稱 Department |
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畢業學年期 Year, semester |
語文別 Language |
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學位類別 Degree |
頁數 Number of pages |
39 |
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研究生 Author |
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指導教授 Advisor |
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召集委員 Convenor |
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口試委員 Advisory Committee |
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口試日期 Date of Exam |
2006-05-29 |
繳交日期 Date of Submission |
2006-06-05 |
關鍵字 Keywords |
肺癌、致癌基因、食道癌 BRAF gene, esophageal cancer, Lung cancer |
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統計 Statistics |
本論文已被瀏覽 5681 次,被下載 2281 次 The thesis/dissertation has been browsed 5681 times, has been downloaded 2281 times. |
中文摘要 |
RAF-MEK-ERK是控制細胞增殖、分化和存亡的重要訊息傳遞路徑。最近的報導指出,B-RAF的突變在人類癌症中有很高的發生率, B-RAF發生突變的exon 11和 exon 15位置分別在glycine-rich loop和活化作用區。在B-RAF突變中, V600E是在人類癌症中最常發現的突變位點,包括黑色素細胞癌及甲狀腺癌。在此次的論文研究,我們取得86例的癌症,其中包括62例的肺癌與24例的食道癌,進行聚合酶連鎖反應(PCR)放大BRAF exon 11和15以及對DNA序列進行突變之分析。在分析的結果中我們並沒有在這兩個exon中發現任何的突變,這顯示BRAF的突變在肺癌及食道癌中可能較少,而這樣的結論還需要更多的樣品來分析證實。 |
Abstract |
The RAF-MEK-ERK is an important signaling pathway that controls cellular proliferation, differentiation and survival. Recent reports indicate that R-RAF is mutated at a high frequency in human cancer. The mutations are clustered in the glycine-rich loop and activation segment which are encoded by exon 11 and exon 15, respectively. Among these mutations, V600E is the most prevalent found in varieties of human cancers, include melanoma and thyroid carcinomas. In this thesis, we analyzed 86 human cancer specimens, including 62 lung cancers and 24 esophageal cancers, for the mutation of exons 11 and 15 by PCR and direct DNA sequencing. However, we can not detect any mutation in these two exons in these clinical samples, these results suggest indicating that BRAF mutation might be rare and analysis of larger sample size is needed to confirmed this conclusion. |
目次 Table of Contents |
目 錄 中文摘要 ………………………………………………………………1 英文摘要 ………………………………………………………………2 背景介紹 ………………………………………………………………3 材料……………………………………………………………………15 方法……………………………………………………………………17 結果……………………………………………………………………19 討論……………………………………………………………………21 參考資料………………………………………………………………23 圖表……………………………………………………………………27 |
參考文獻 References |
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