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博碩士論文 etd-0611101-115121 詳細資訊
Title page for etd-0611101-115121
論文名稱
Title
萘乙酸對大豆下胚軸不定根誘發過程中組織內過氧化酶和木質素之影響
Effect of naphthaleneacetic acid on peroxidase and lignin in hypocotyl cutting of soybean during root formation
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
30
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2001-05-31
繳交日期
Date of Submission
2001-06-11
關鍵字
Keywords
不定根、萘乙酸、過氧化酶、木質化作用
peroxidase, naphthaleneacetic acid, lignification, Adventitious root, Glycine max
統計
Statistics
本論文已被瀏覽 5643 次,被下載 1947
The thesis/dissertation has been browsed 5643 times, has been downloaded 1947 times.
中文摘要
人工合成生長素,萘乙酸,在促進大豆下胚軸誘發不定根時,其下胚軸內部陰性和陽性過氧化酶活性降低、過氧化酶 ( pI 4.4,pI 8.3和pI 9.4 ) 基因的表現減少以及木質素含量降低。所以我們推測萘乙酸在促進發根的同時,可能抑制過氧化酶基因的表現。另外,過氧化酶的活性和木質素含量降低,推測除了陰性過氧化酶參與木質化作用之外,陽性過氧化酶可能與木質素的合成亦有密切的關係。
Abstract
Synthetic auxin, naphthaleneacetic acid ( NAA ), promoted rooting in soybean ( Glycine max ) hypocotyls. The activities of anionic peroxidases (PODs, pI 3.5 and pI 3.7 ) and cationic PODs ( pI 8.3, pI 9.1 and pI 9.4 ) in soybean hypocotyls were significantly inhibited by exogenous NAA during the induction of adventitious roots. In addition, the reduction in the activity of PODs ( pI 4.4, pI 8.3 and pI 9.4 ) were correlated with a reduction of their corresponding transcription activity. The PODs patterns and transcription in the NAA-treated hypocotyls demonstrated the specific effect of NAA on POD ioszymes, indicating that NAA might suppress PODs genes expression during the adventitious root formation. The decline of PODs activity in the NAA-treated hypocotyls was accompanied by the decrease of lignin contents. We suggest that both of anionic and cationic PODs might be in part responsible for lignin synthesis in soybean hypocotyls.
目次 Table of Contents
TABLE OF CONTENTS

Chinese abstract -------------------------------- 3

English abstract -------------------------------- 4

Table of contents ------------------------------- 5

List of tables and figures ---------------------- 6

Introduction ------------------------------------ 7

Materials and Methods --------------------------- 10

Results ----------------------------------------- 15

Discussion -------------------------------------- 18

Reference --------------------------------------- 20

Tables and figures ------------------------------ 24


參考文獻 References
References
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