論文使用權限 Thesis access permission:校內校外完全公開 unrestricted
開放時間 Available:
校內 Campus: 已公開 available
校外 Off-campus: 已公開 available
論文名稱 Title |
萘乙酸對大豆下胚軸不定根誘發過程中組織內過氧化酶和木質素之影響
Effect of naphthaleneacetic acid on peroxidase and lignin in hypocotyl cutting of soybean during root formation |
||
系所名稱 Department |
|||
畢業學年期 Year, semester |
語文別 Language |
||
學位類別 Degree |
頁數 Number of pages |
30 |
|
研究生 Author |
|||
指導教授 Advisor |
|||
召集委員 Convenor |
|||
口試委員 Advisory Committee |
|||
口試日期 Date of Exam |
2001-05-31 |
繳交日期 Date of Submission |
2001-06-11 |
關鍵字 Keywords |
不定根、萘乙酸、過氧化酶、木質化作用 peroxidase, naphthaleneacetic acid, lignification, Adventitious root, Glycine max |
||
統計 Statistics |
本論文已被瀏覽 5643 次,被下載 1947 次 The thesis/dissertation has been browsed 5643 times, has been downloaded 1947 times. |
中文摘要 |
人工合成生長素,萘乙酸,在促進大豆下胚軸誘發不定根時,其下胚軸內部陰性和陽性過氧化酶活性降低、過氧化酶 ( pI 4.4,pI 8.3和pI 9.4 ) 基因的表現減少以及木質素含量降低。所以我們推測萘乙酸在促進發根的同時,可能抑制過氧化酶基因的表現。另外,過氧化酶的活性和木質素含量降低,推測除了陰性過氧化酶參與木質化作用之外,陽性過氧化酶可能與木質素的合成亦有密切的關係。 |
Abstract |
Synthetic auxin, naphthaleneacetic acid ( NAA ), promoted rooting in soybean ( Glycine max ) hypocotyls. The activities of anionic peroxidases (PODs, pI 3.5 and pI 3.7 ) and cationic PODs ( pI 8.3, pI 9.1 and pI 9.4 ) in soybean hypocotyls were significantly inhibited by exogenous NAA during the induction of adventitious roots. In addition, the reduction in the activity of PODs ( pI 4.4, pI 8.3 and pI 9.4 ) were correlated with a reduction of their corresponding transcription activity. The PODs patterns and transcription in the NAA-treated hypocotyls demonstrated the specific effect of NAA on POD ioszymes, indicating that NAA might suppress PODs genes expression during the adventitious root formation. The decline of PODs activity in the NAA-treated hypocotyls was accompanied by the decrease of lignin contents. We suggest that both of anionic and cationic PODs might be in part responsible for lignin synthesis in soybean hypocotyls. |
目次 Table of Contents |
TABLE OF CONTENTS Chinese abstract -------------------------------- 3 English abstract -------------------------------- 4 Table of contents ------------------------------- 5 List of tables and figures ---------------------- 6 Introduction ------------------------------------ 7 Materials and Methods --------------------------- 10 Results ----------------------------------------- 15 Discussion -------------------------------------- 18 Reference --------------------------------------- 20 Tables and figures ------------------------------ 24 |
參考文獻 References |
References Aeschbacher RA, Schiefelbein JW, Benfey PN ( 1994 ) The genetic and molecular basis of root development. Annual Review of Plant Physiology and Plant Molecular Biology 45, 25-45. Bernards MA, Fleming WD, Llewellyn DB, Priefer R, Yang X, Sabatino A, Plourde GL ( 1999 ) Biochemical characterization of the suberization- associated anionic peroxidase of potato. Plant Physiology 121, 135-145. Boudet AM ( 1998 ) A new view of lignification. Trends in Plant Science 3, 67-71. Boudet AM ( 2000 ) Lignins and lignification: Selected issues. Plant Physiology biochemistry 38, 81-96. Bruce R, West CA ( 1989 ) Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean. Plant Physiology 91, 889-897. Carpin S, Crèvecoeur M, Greppin H, Penel C ( 1999 ) Molecular cloning and tissue-specific expression of an anionic peroxidase in zucchini. Plant Physiology 120, 799-810. Christensen JH, Bauw G, Welinder KG, Montagu MV, Boerjan W ( 1998 ) Purification and characterization of peroxidases correlated with lignification in poplar xylem. Plant Physiology 118, 125-135. Fett-Neto AG, Teixeira SL, Dasilva, EAM, Anna RS ( 1992 ) Biochemical and morphological changes during in vitro rhizogenesis in cuttings of Sequoia sempervirens (D. don ) Endi. Journal of Plant Physiology 140, 720-728. Friedman R, Altman A, Zamski E ( 1979 ) Adventitious root formation in bean hyopcotyl cuttings in relation to IAA translocation and hypocotyl anatomy. Journal of Experimental Botany 30, 768-777. Gazaryan IG, Chubar TA, Mareeva EA, Lagrimini LM, Huystee RBV, Thorneley RNF ( 1999 ) Aerobic oxidation of indole-3-acetic acid catalyzed by anionic and cationic peanut peroxidase. Phytochemistry 51, 175-186. González LF, Perez F, Rojas MC ( 1999 ) Indole-3-acetic acid control on acidic oat cell wall peroxidases. Journal of Plant Growth Regulation 18, 25-31. Iiyama K, Lam TB-T, Stone BA ( 1994 ) Covalent cross-links in the cell wall. Plant Physiology 104, 315-320. Klotz KL, Lagrimini LM ( 1996 ) Phytohormone control of the tobacco anionic peroxidase promoter. Plant Molecular Biology 31, 565-573. Kristensen BK, Bloch H, Rasmussen SK ( 1999 ) Barley coleoptile peroxidases. Purification, molecular cloning, and induction by pathogens. Plant Physiology 120, 501-512. Lagrimini LM ( 1991 ) Wound-induced deposition of polyphenols in transgenic plants overexpressing peroxidase. Plant Physiology 96, 577-583. Lagrimini LM, Rothstein S ( 1987 ) Tissue specificity of tobacco peroxidase isozymes and their induction by wounding and tobacco mosaic virus infection. Plant Physiology 84, 438-442. Li T-C, Feng T-Y, Chen W-S, Liu Z-H ( 2001 ) The acute effect of copper on the levels of indole-3-acetic acid and lignin in peanut roots. Australian Journal of Plant Physiology 28, 1-6. Liu Z-H, Hsiao I-C, Pan Y-W ( 1996 ) Effect of naphthaleneacetic acid on endogenous indole-3-acetic acid, peroxidase and auxin oxidase in hypocotyl cuttings of soybean during root formation. Botanical Bulletin of Academia Sinica 37, 247-253. McDougall GJ, Stewart D, Morrison IM ( 1994 ) Cell-wall-bound oxidase from tobacco ( Nicotiana tabccum ) xylem participate in lignin formation. Planta 194, 9-14. Müsel G, Schindler T. Bergfeld R, Ruel K, Jacquet G. Lapierre C, Speth V, Schoptfer P ( 1997 ) Structure and distribution of lignin in primary and secondary cell walls of maize coleoptiles analyzed by chemical and immunological probes. Planta 201, 146-159. Normanly J, Slovin JP, Cohen JD ( 1995 ) Rethinking auxin biosynthesis and metabolism. Plant Physiology 107, 323-329. Quiroga M, Guerrero C, Botella MA, Barceló A, Amaya I, Medina MI, Alonso FJ, Forchetti SM, Tigier H, Valpuesta V ( 2000 ) A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiology. 122, 1119-1127. Sagee O, Raviv MM, Becker D, Cosse A ( 1992 ) Involvement of rooting factors and free IAA in the rootability of citrus species stem cuttings. Science Horticulture 51, 187-195. Sato Y, Sugiyama M, Górecki RJ, Fukuda H, Komamine A ( 1993 ) Interrelationship between lignin deposition and the activities of peroxidase isoenzymes in differentiating tracheary elements of Zinnia. Planta 189, 584-589. Sterjiades R, Dean JFD, Gamble G, Himmelsbach DS, Eriksson K-EL ( 1993 ) Extracellular laccases and peroxidases from sycamore maple ( Acer pseudoplatanus ) cell-suspension cultures. Planta 190, 75-87. Wang W-C, Liu Z-H ( 1999 ) Harpinpss-induced peroxidase and lignin accumulation I tobacco during the hypersensitive response. Australian Journal of Plant Physiolog. 26, 265-272. Wojtaszek P ( 1997 ) Oxidative burst: an early plant response to pathogen infection. Biochemistry Journal. 322, 681-692. |
電子全文 Fulltext |
本電子全文僅授權使用者為學術研究之目的,進行個人非營利性質之檢索、閱讀、列印。請遵守中華民國著作權法之相關規定,切勿任意重製、散佈、改作、轉貼、播送,以免觸法。 論文使用權限 Thesis access permission:校內校外完全公開 unrestricted 開放時間 Available: 校內 Campus: 已公開 available 校外 Off-campus: 已公開 available |
紙本論文 Printed copies |
紙本論文的公開資訊在102學年度以後相對較為完整。如果需要查詢101學年度以前的紙本論文公開資訊,請聯繫圖資處紙本論文服務櫃台。如有不便之處敬請見諒。 開放時間 available 已公開 available |
QR Code |