汞是一種劇毒的元素，破壞中樞神經系統，對口、粘膜和牙齒有不良影響，於水中無色無味，非常難以察覺，而其化合物被廣泛應用於工業產品中，因此對於水質汙染機會就更高了，所以希望能發展出一個能做到快速靈敏的檢驗方法，在此使用磁性奈米粒子進行檢測，藉由強磁性可以做到一個快速分離，可以搭配質譜儀來做到快速檢驗的功能，而本次實驗所合成出的磁性奈米粒子具有良好的專一性，而且能於真實樣品中測得0.1fmole的汞離子濃度，對於每次實驗所需的測量時間不需超過五分鐘，也不需加入太多基質或是做其他前處理，就可以在如海水等基質複雜中樣品做檢驗。本次實驗利用磁性奈米粒子來做為SALDI(Surface-assisted laser desorption/ionization表面輔助雷射脫附游離法)來對於汞離子進行偵測，將磁性奈米粒子搭配MALD-MSI來進行對於汞離子的偵測，利用磁性奈米物質可以做到快速分離再進入到MALDI-MS進行偵測，以檢測環境中的水質汙染程度，因此用Thymine@Chitosan@Fe3O4這個磁性奈米粒子對於Hg有良好的結合性以進行分離，再使用MALDI-MS進行檢驗，因為使用此材料來做為SALDI-MS基質，藉由Thymine與Hg有良好的鍵結力，再利用磁場做分離進行檢測，開發一個快速且靈敏的分析方法，可以有效對於Hg來進行環境分析。

Mercury is a highly toxic element and it will cause damage to the central nervous system. Because it is colorless and tasteless in water, so it is hard to detect. It’s compounds which are widely used in industrial products that enhances the opportunity of water pollution. The reported methods are hard to make the method fast and selective. We use thymine modified chitosan magnetic nanoparticles(TCTS) combined with LDI-MS(laser desorption/ionization Mass Spectrometry) as SALDI-MS(Surface-assisted laser desorption/ionization Mass Spectrometry) to show good specificity and sensitivity to the Hg2+ ions. Because Thymine and Hg can easy get binding, so we use TCTS serves as a probe to detect Hg2+ ions. We use magnetic field to separate and then use LDI-MS to test the sample. We can measure Hg2+ ions(0.1ppb) in environmental samples such as tap and sea water in a very short time < 5 min. TCTS combined with LDI-MS offers a sensitive and selective method that can provide simple, inexpensive, fast test of Hg2+ ions in water.

第一章.簡介 1
1.1奈米科技 1
1.1.1發展歷史 1
1.1.2特性 2
1.1.3 合成 3
1.2磁性奈米粒子 4
1.2.1特性 4
1.2.2 應用 5
1.3 Thymine 7
1.3. MALDI 8
1.3.1歷史 8
1.3.2 原理 9
1.4汞 10
1.4.1汞中毒 10
1.4.2檢驗 11
1.5 研究目標 12
參考資料 13
第二章、使用胸線嘧啶修飾過的磁性奈米粒子對於溶液中汞離子進行專一性結合感測 17
1.前言 18
2.介紹 19
3.實驗與步驟 20
1.藥品 20
2.儀器 21
3.TCTS奈米粒子的合成 21
4.選擇性的探討 22
5.靈敏度的探討 22
6.真實樣品的分析 22
4.結果與討論 23
1.材料的鑑定 23
2.靈敏度和選擇性 28
3.真實樣品分析 39
總結 45
參考文獻 47

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