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博碩士論文 etd-0619103-133648 詳細資訊
Title page for etd-0619103-133648
論文名稱
Title
鉀離子通道結合蛋白在二價離子誘導下構型影響及聚合作用之研究
Divalent cation-induced conformational changes and oligomerization of KChIP1
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
66
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2003-06-10
繳交日期
Date of Submission
2003-06-19
關鍵字
Keywords
聚合作用、鉀離子通道、構型變化
KChIP, conformational change, oligomerization
統計
Statistics
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The thesis/dissertation has been browsed 5637 times, has been downloaded 6802 times.
中文摘要
摘要
KChIPs family為鉀離子通道結合蛋白,可與電位驅動型鉀離子通道(Kv4)
Abstract
Abstract

KChIPs are Kv channel-interacting proteins that bind to the cytoplasmic N-terminus of Kv4
目次 Table of Contents
目錄
頁次
壹、中文摘要……….………...……………………………………….1
貳、英文摘要…………….….………………………………………...2
參、縮 寫……………..…….……………………………………..4
肆、緒 論.…………….….………………………………………..5
伍、材 料……….…….………………………………………….11
陸、實驗方法….………….…….……………………………………13
cDNA端點快速放大……………………………………………………..13
去氧核醣核酸序列分析……...…………………………………………..14
表現載體之建構……………...…………………………………………..14
基因轉型………………...………………………………………………..16
重組蛋白質的大量表現…………...……………………………………..17
蛋白質定量…………………...…………………………………………..18
SDS-PAGE…………………...…………………………………………...18
螢光測定……………………………...…………………………………..19
偏極光譜分析…………………...………………………………………..20
鈣離子結合能力之測定……………...…………………………………..20
蛋白質聚合反應……………...…………………………………………..21
Pull down assay……………………...…………………………………....21
西方墨點法….…………....………………………………………………22
DNA結合能力之分析…….…………….…………………………….…22
NATIVE-PAGE…………………...………………………………………23
柒、實驗結果……….……….……………………………………….24
KChIPs cDNA之選殖……………………………………………………24
重組蛋白質之表現與純化………...……………………………………..24
螢光測定………………….…..…………………………………………..26
鈣離子結合能力分析…………...………………………………………..27
二級結構圖譜分析……………………...………………………………..28
蛋白質分子間交互作用………………...………………………………..28
DRE結合能力分析……………….……………………………………..30
His-KChIP重組蛋白之抗原性偵測…………………………………….30
捌、討 論.…….……….…………………………………………31
玖、圖 表…………...……………………………………………37
拾、參考文獻…………………...……………………………………61

參考文獻 References
參考文獻
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