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論文名稱 Title |
鉀離子通道結合蛋白在二價離子誘導下構型影響及聚合作用之研究
Divalent cation-induced conformational changes and oligomerization of KChIP1 |
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系所名稱 Department |
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畢業學年期 Year, semester |
語文別 Language |
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學位類別 Degree |
頁數 Number of pages |
66 |
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研究生 Author |
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指導教授 Advisor |
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召集委員 Convenor |
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口試委員 Advisory Committee |
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口試日期 Date of Exam |
2003-06-10 |
繳交日期 Date of Submission |
2003-06-19 |
關鍵字 Keywords |
聚合作用、鉀離子通道、構型變化 KChIP, conformational change, oligomerization |
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統計 Statistics |
本論文已被瀏覽 5637 次,被下載 6802 次 The thesis/dissertation has been browsed 5637 times, has been downloaded 6802 times. |
中文摘要 |
摘要 KChIPs family為鉀離子通道結合蛋白,可與電位驅動型鉀離子通道(Kv4) |
Abstract |
Abstract KChIPs are Kv channel-interacting proteins that bind to the cytoplasmic N-terminus of Kv4 |
目次 Table of Contents |
目錄 頁次 壹、中文摘要……….………...……………………………………….1 貳、英文摘要…………….….………………………………………...2 參、縮 寫……………..…….……………………………………..4 肆、緒 論.…………….….………………………………………..5 伍、材 料……….…….………………………………………….11 陸、實驗方法….………….…….……………………………………13 cDNA端點快速放大……………………………………………………..13 去氧核醣核酸序列分析……...…………………………………………..14 表現載體之建構……………...…………………………………………..14 基因轉型………………...………………………………………………..16 重組蛋白質的大量表現…………...……………………………………..17 蛋白質定量…………………...…………………………………………..18 SDS-PAGE…………………...…………………………………………...18 螢光測定……………………………...…………………………………..19 偏極光譜分析…………………...………………………………………..20 鈣離子結合能力之測定……………...…………………………………..20 蛋白質聚合反應……………...…………………………………………..21 Pull down assay……………………...…………………………………....21 西方墨點法….…………....………………………………………………22 DNA結合能力之分析…….…………….…………………………….…22 NATIVE-PAGE…………………...………………………………………23 柒、實驗結果……….……….……………………………………….24 KChIPs cDNA之選殖……………………………………………………24 重組蛋白質之表現與純化………...……………………………………..24 螢光測定………………….…..…………………………………………..26 鈣離子結合能力分析…………...………………………………………..27 二級結構圖譜分析……………………...………………………………..28 蛋白質分子間交互作用………………...………………………………..28 DRE結合能力分析……………….……………………………………..30 His-KChIP重組蛋白之抗原性偵測…………………………………….30 捌、討 論.…….……….…………………………………………31 玖、圖 表…………...……………………………………………37 拾、參考文獻…………………...……………………………………61 |
參考文獻 References |
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