綠島產紫羽珊瑚(Clavularia violacea Quoy and Gaimard)之有機層萃取物經細胞活性測試，發現對P-388、HT-29及A-549三種癌細胞有毒殺活性，以癌細胞毒殺活性為指標，將有機層之萃取物以矽膠管柱層析及HPLC進行分離純化，得到一種prostanoid (1)、兩種cembranoid diterpenes (2及3)、一種aromadedrane sesquiterpene (4)、一種neodollabellane diterpene (5)、(+)-curcuphenol (6) 及兩種sesquiterpene lactones (7及8)。其中1，2，4，7及8是新化合物。化合物1- 5具有很強的癌細胞毒殺活性。

The methylene chloride and acetone extracts of Formosan soft coral Clavularia violacea (Collected at Green Island) were found to exhibit significant cytotoxicity against P-388, A-549 and HT-29 cancer cell lines. Chromatographic separation let to the isolation of one prostanoid, 1, two cembranoid deterpenes, 2 and 3, one aromadedrane-type sesquiterpene, 4, one neodollabellane diterpene, 5, (+)-curcuphenol, 6 and two sesquiterpene lactones, 7 and 8. Compounds 1, 2, 4, and were new compounds. Compounds 1-5 exhibited significant cytotoxicity against P-388, HT-29 and A-549 cancer cell.

壹、緒論 9
一、前言 9
二、Clavularia屬軟珊瑚之天然物化研究回顧 10-24
三、研究動機與目的 25
貳、紫羽珊瑚化學成分之研究 26
一、材料採集與鑑定 26
二、紫羽珊瑚之分類地位 26
三、紫羽珊瑚之萃取與分離流程 27-28
四、紫羽珊瑚化學成分之分離純化 29
(1) GN33-235 (1)之分離純化 29-31
(2) GN33-195 (2)之分離純化 32-34
(3) GN33-133 (3)之分離純化 35-37
(4) GN33-258 (4)之分離純化 38-39
(5) GN33-004 (5)之分離純化 40-42
(6) GN33-032 (6)之分離純化 43-45
(7) GN33-124 (7)之分離純化 46-48
(8) GN33-218 (8)之分離純化 49-51
五、紫羽珊瑚化學成分之結構解析 52
(1) GN33-235 (1)之結構解析 52-54
(2) GN33-195 (2)之結構解析 56-58
(3) GN33-133 (3)之結構解析 60-62
(4) GN33-258 (4)之結構解析 64-65
(5) GN33-004 (5)之結構解析 67-69
(6) GN33-032 (6)之結構解析 71-72
(7) GN33-124 (7)之結構解析 74-76
(8) GN33-218 (8)之結構解析 78-80
參、紫羽珊瑚之生物活性 82
肆、結論 83
伍、參考文獻 84-90
陸、附錄 92
一、 儀器及材料 92-93
二、 癌細胞毒殺活性測試之分析方法 94
三、 MTT分析原理 95

圖一、軟珊瑚(Clavularia violacea)之分離流程 28
圖二、GN33-235 (1)之1D數據 30-31
圖三、GN33-195 (2)之1D數據 33-34
圖四、GN33-133 (3)之1D數據 36-37
圖五、GN33-258 (4)之1D數據 39
圖六、GN33-004 (5)之1D數據 41-42
圖七、GN33-032 (6)之1D數據 44-45
圖八、GN33-124 (7)之1D數據 47-48
圖九、GN33-218 (8)之1D數據 50-51
圖十、GN33-235 (1)之2D關連圖 55
圖十一、GN33-195 (2)之2D關連圖 59
圖十二、GN33-133 (3)之2D關連圖 63
圖十三、GN33-258 (4)之2D關連圖 66
圖十四、GN33-004 (5)之2D關連圖 70
圖十五、GN33-032 (6)之2D關連圖 73
圖十六、GN33-124 (7)之2D關連圖 77
圖十七、GN33-218 (8)之2D關連圖 81

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