本研究探討石斑魚（Epinepheus malabaricus）飼料中添加高濃度的維他命E對魚體成長、組成與各種健康指標之影響。實驗飼料五種是由以酪蛋白為蛋白源配製成的基礎飼料，分別添加五個濃度維他命E（all-rac-a-tocopherol）0、200、400、1000及3000mg/kg diet 。石斑魚稚魚（平均體重30g）養殖於室內封閉式循環流水系統，每魚缸6尾魚，每飼料組均有三重覆。經過十七週餵飼不同維他命E含量飼料，魚體增重率間（170-250%）並沒有顯著的差異。維他命E添加量與石斑魚體肝臟粗脂肪含量成正比，而與粗蛋白含量成反比，魚體肌肉脂質的極性脂肪酸w-3 HUFA隨著維他命E添加量增加而降低。餵飼實驗進行到第11週時，未添加組石斑魚每次攝食時魚體出現捕食動作及張口攝餌卻沒吃到餌料的失誤率，顯著高於其它各組，但當維他命E添加量達到400mg/kg後各添加組攝食失誤率間則無差異，顯示維他命E缺乏會負面影響石斑魚的攝食。維他命E添加亦影響肝臟天門冬胺酸轉胺酵素（AST）活性，添加量最低與最高（0與3000 mg/kg）兩組顯著高於其它三組，組織學切片顯示低濃度組肝細胞有空胞化的情形發生。魚體肌肉過氧化指標TBARS濃度隨著飼料維他命E添加量的增加而降低。高濃度維他命E的添加對馬拉巴石斑稚魚免疫能力的提昇似乎相當有限，在各項免疫學檢測中，除血清凝集力價在高濃度組（3000 mg/kg）顯著較高，溶菌酵素與超氧岐化酵素在200與400 mg/kg組間有最佳的活性外，巨噬細胞內超氧陰離子分泌量及補體活性皆不受維他命E添加影響。

This research determined the influence of dietary vitamin E levels on the growth, body composition and various health indices of juvenile Epinephelus malabaricus. Five experimental diets were formulated by adding all-rac-a-tocopherol in 0, 200, 400, 1000 and 3000mg/kg feed to the basal feed that used casein as the sole protein source. The juveniles, having an average weight of 30g, were cultivated in an indoor closed recirculation system, with 6 fishes in each tank, and 3 tanks for each experimental feed. After the 17-week feeding trial, weight gain of the fish (170-250%) was not significantly affected by the vitamin E treatments. The supplemental level of vitamin E was positively related to the lipid levels in liver, but was negatively related to the protein levels in liver. Total w-3 HUFA of polar lipids in body muscle were decreased with the increasing supply of vitamin E. It was observed on the 11th week and beyond that the fish fed with the vitamin-free diet were prone to fail in taking in feed particles although their responses to feeding were of no difference to the supplemental groups. But once the supplemental levels of vitamin E were higher than 400mg/kg no difference in feeding failure was detected, indicating deficiency of vitamin E detrimentally affected fish feeding. Plasma AST activities in the 0 and 3000mg/kg groups were significantly higher than those of the other groups. Histological sections of fish liver indicated that liver cells in low vitamin E groups showed signs of vacuolization. TBARS (thiobarbituric acid reactive substances) decreased with the increasing vitamin E levels in the feed. Vitamin E showed limited enhancement on the immune parameters studied. Except that agglutination titter was significantly higher in fish fed the high vitamin E diet (3000mg/kg) and serum lysozyme and erythrocyte SOD showed highest activity in 200 and 400mg/kg groups, intracellular super oxide anion of macrophages and serum alternative complement pathway activities were not significantly affected by vitamin E supplementation.

中文摘要……………………………………………………………. I
英文摘要……………………………………………………………. III
目錄…………………………………………………………………. V
表目錄………………………………………………………………. VI
圖目錄………………………………………………………………. VIII
文獻回顧……………………………………………………..……… 1
前言…………………………………………………………………. 11
材料與方法…………………………………………………………. 13
結果…………………………………………………………………. 34
討論………………………………………………………………….. 59
參考文獻……………………………………………………………. 65

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