一氧化碳（carbon monoxide, CO）在腦中被認為是一內生性的生物訊息分子。原血紅素氧化酶（heme oxygenase, HO）分解原血紅質（heme）而產生一氧化碳與膽綠素。在腦中，一氧化碳為一神經傳導物質與神經元的訊息分子。我們過去曾發現一氧化碳參與了中樞心臟血管調控作用與感壓反射的作用，而且許多研究指出一氧化碳會影響麩氨酸的神經傳導作用，進一步的研究則發現活化 metabotropic 麩氨酸接受器（metabotropic glutamate receptors, mGluRs）與原血紅素氧化

Carbon monoxide (CO) has been identified as an endogenous biological messenger in the brain. Heme oxygenase (HO) catalyzes the metabolism of heme to CO and biliverdin. CO has been shown to act as a neurotransmitter and neuronal messenger in the brain. We reported recently that CO was involved in central cardiovascular regulation, modulated the baroreflex, may affect glutamatergic neurotransmission, and metabotropic glutamate receptors (mGluRs) may be coupled to the activation of HO in the nucleus tractus solitarii (NTS) of rats. We also reported previously that adenosine can increase the release of glutamate in the NTS. The present study was designed to investigate the possible interaction of CO, adenosine, and mGluRs groups in the NTS. Male Sprague-Dawley rats were anesthetized with urethane, and blood pressure were monitored intra-arterially. Unilateral microinjection of ascending doses of hemin (0.01 to 3.3 nmol), a heme molecule cleaved by HO to yield CO, produced decreases in blood pressure and heart rate dose-dependently. In addition, similar cardiovascular effects were observed in adenosine (2.3 nmol) and several agonists for mGluRs groups such as DHPG (group Ⅰ) (0.03 nmol), APDC (group Ⅱ) (0.3 nmol)and L-AP4 (group Ⅲ) (0.3 nmol). These cardiovascular effects of hemin were attenuatd by prior administration of the adenosine receptor antagonist DPSPX (0.92 nmol). Similarly, pre-treatment of HO inhibitor ZnPPⅨ or ZnDPBG (1 nmol) also attenuated the depressor and bradycadic effects of adenosine. Among the mGluRs agonists, prior administration of ZnPPⅨ (1 nmol), an inhibitor of HO activity, significantly attenuated the cardiovascular effects of APDC and L-AP4, and failed to prevent the cardiovascular responses of DHPG. These results indicated an interaction between CO and adenosine, and group Ⅱ and Ⅲ mGluRs may be coupled to the activation of HO in central cardiovascular regulation.

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