迷走感覺神經的呼吸道支配與調控呼吸道神經性發炎反應有密切關係。一般常利用辣椒素來做感覺神經刺激作為痛覺及發炎相關研究，其特性能專一性刺激感覺傳入神經，引起呼吸道神經性發炎反應。在過去的研究我們發現切斷迷走神經的分支，胸迷走神經或喉返神經，經由靜脈注射辣椒素 (300 nmol/ml/kg)引起下呼吸道不同程度減弱的發炎反應。為釐清喉返神經與胸迷走神經在氣管的支配，本實驗利用辣椒素3μl (10 mg/ml)直接注射於胸迷走神經幹或胸迷走分支並做不同迷走神經分支的神經切斷手術後，並立即注射India ink 當做神經性發炎引起呼吸道血管血漿外洩的標定劑，15分鐘後灌流，結果顯示辣椒素注射於胸迷走神經幹會引起下呼吸道嚴重發炎反應 (22%)，喉返神經的切斷明顯抑制了氣管上半部的發炎反應 (左側減少77.6%，右側減少 84.5%)，而胸迷走神經的切斷則無顯著減弱，此結果指出迷走神經支配到氣管上半部主要是經由喉返神經而來。
此外辣椒素刺激迷走神經幹亦引起喉頭嚴重發炎反應，將上喉神經及喉返神經一起切斷後，India ink標定血漿外洩的血管密度明顯降低84.98%，個別切斷胸迷走神經與喉返神經也有減弱的跡象，此結果暗示迷走神經下行的分支，上喉神經、喉返神經及胸迷走神經的感覺軸突很可能來自同一迷走神經節神經元。

Vagal sensory afferent innervation corresponds to regulation of neurogenic inflammation in the airways. Capsaicin is mostly used for stimulation of sensory nerves that induce pain and inflammatory responses. It can specifically stimulate sensory afferent nerves, inducing neurogenic inflammation in the airways. According the past studies, we have found the right thoracic vagus nerve (RTVN) and right recurrent laryngeal nerve (RRLN); branches of right thoracic vagus trunk (RTVT) mediate different degree of neurogenic inflammation by intraenous injection of capsaicin (300 nmol/ml/kg). In order to investigate the innervation from the RTVN and RRLN of rat tracheobronchi and their involvement in plasma exudation, we injected 3 μl of capsaicin (10 mg/ml) into RTVT and denervated the RRLN or RTVN and used India ink as tracer dye to label the leaky microvessels. Our observation indicated that injection of capsaicin into the RTVT coud induce obvious plasma exudation in trachea (area density of leaky blood vessels was about 22%), but plasma exudation was significantly decreased after denervation of RRLN. The left upper side of trachea was decreased by 77.6% and the right upper side decreased by 84.5%. This phenomenon was not caused by denervation of RTVN. The results suggest that vagal nerve innervation of upper trachea mostly came from the RLN.

Otherwise, capsaicin injection into the RTVT also induced neurogenic inflammation in the larynx. Experimental denervation of both superior and recurrent laryngeal nerves resulted in a decrease of plasma extravasation by 84.98%. Denervation of either RTVN or RRLN also decreased the plasma extravasation in the larynx. The evidence suggest that sensory fibers in the superior laryngeal nerve, recurrent laryngeal nerve, and thoracic vagus nerve might come from the same population of vagal ganglion sensory neurons.

緒言(Introduction)……………………………………………1
研究目的(Purpose of study)…………………………………7
材料與方法(Materials and Methods)……………………….8
結果(Results)…………………………………………………17
討論(Discussions)……………………………………………21
參考文獻(References)……………………………………….25
圖……….……………………………………………………..31
Tables………………………………………………………….36

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