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博碩士論文 etd-0622103-184409 詳細資訊
Title page for etd-0622103-184409
論文名稱
Title
臺灣產小孔蛸(Cistopus sp.)之黏液囊形態及分類學研究
Morphology of the Mucous Pouches and Taxonomic Study of Cistopus sp. (Cephalopoda: Octopodidae) from Taiwan
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
61
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2003-06-10
繳交日期
Date of Submission
2003-06-22
關鍵字
Keywords
章魚、分類學、組織學、頭足類、親緣關係
systematics, octopus, histology, phylogeny, Cephalopoda
統計
Statistics
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The thesis/dissertation has been browsed 5707 times, has been downloaded 2268 times.
中文摘要
小孔蛸(Cistopus sp.)是一種中到大型的章魚,在臺灣的魚市場是很常見的種類。小孔蛸屬最重要的特徵是在各個腕間膜的基部具有一個黏液囊的構造,然而卻沒有關於這個特殊構造的研究。本實驗除了描述臺灣海域小孔蛸的形態特徵之外,並以組織切片的方法觀察其黏液囊的構造,而粒線體 COI 基因則用來分析小孔蛸與章魚科其他屬之間的親緣關係。根據本研究的結果顯示,臺灣海域的小孔蛸與真正的印尼小孔蛸和安達曼海域的小孔蛸不同。臺灣的此種小孔蛸廣泛分布在亞洲大陸沿岸到印度海域,並且在黏液孔有性雙型的情形。黏液囊的內壁由柱狀上皮細胞所構成,黏液就是由這些上皮細胞所分泌,而黏液囊內壁的物質則是酸性的黏多醣類。由章魚 COI DNA 序列所作的親緣關係分析,結果顯示小孔蛸與其他章魚亞科的成員形成一個單系群。
Abstract
Cistopus sp. is a medium to large octopod and it is common in the fish markets of Taiwan. The diagnostic character of the genus Cistopus is the possession of eight mucous pouches in the web between the base of each arm. However, researches on this special structure were absent. This study is to describe the morphological characters of Cistopus sp. from Taiwanese waters and to examine the mucous pouch histologically. The mitochondrial COI gene sequences are used as a tool to analyze the phylogenetic relationships between Cistopus and other genera of the family Octopodidae. Based on the results of this study, the Cistopus of Taiwanese waters is different from the genuine C. indicus and the Cistopus of the Andaman Sea. Cistopus sp. is widely distributed from coastal Asia to India at least and presents sexual dimorphism in the openings of the mucous pouches. The inner wall of the mucous pouches is composed of columnar epithelial cells, and the mucus is secreted from the epithelial cells. The substance secreted from the inner wall is acid mucopolysaccharides. According to the phylogenetic analysis of the octopods COI DNA sequences, Cistopus is grouped with other members of subfamily Octopodinae to form a monophyletic group.
目次 Table of Contents
Acknowledgments in Chinese......................................................................................................i
Abstract in Chinese....................................................................................................................ii
Abstract in English....................................................................................................................iii
Table of Contents......................................................................................................................iv
List of Figures.............................................................................................................................v
List of Tables.............................................................................................................................vi

1. Introduction............................................................................................................................1
2. Materials and Methods..........................................................................................................5
2.1 Specimens collection.......................................................................................................5
2.2 Morphological methods..................................................................................................5
2.3 Histological methods......................................................................................................6
2.4 DNA extraction, PCR amplification, cloning, and sequencing.......................................7
2.5 Phylogenetic analysis.....................................................................................................8
3. Results..................................................................................................................................11
3.1 Taxonomic description.................................................................................................11
3.2 Morphology of the mucous pouches...........................................................................13
3.3 Phylogenetic inferences................................................................................................14
4. Discussion............................................................................................................................17
4.1 Remarks on Cistopus....................................................................................................17
4.2 Morphology and function of the mucous pouches......................................................19
4.3 Phylogenetic inferences................................................................................................20
5. Conclusions..........................................................................................................................23
6. References.............................................................................................................................24
Figures......................................................................................................................................31
Tables.......................................................................................................................................45
Appendix..................................................................................................................................53
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