Title page for etd-0622106-154652


[Back to Results | New Search]

URN etd-0622106-154652
Author Hua Chuang
Author's Email Address No Public.
Statistics This thesis had been viewed 4598 times. Download 1946 times.
Department Biological Sciences
Year 2005
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Alteration of Innate Immune Reaction in Patients with Type 2 Diabetes Mellitus
Date of Defense 2006-05-17
Page Count 45
Keyword
  • SOCS-1
  • TLR-4
  • TNF-α
  • Abstract Diabetes mellitus (DM) is the 4th leading cause of mortality in
    Taiwan. Chronic persistent inflammation as demonstrated by higher
    proinflammatory mediators in blood has been correlated to cardiovascular
    complications of type 2 DM. The cellular and molecular mechanism of
    chronic inflammation in type 2 DM remains to be determined. This study
    was conducted to explore altered innate immunity in toll-like receptor
    (TLR) expression and signaling of monocytes from type 2 DM patients.
    Blood leukocytes from type 2 DM patients were counted and studied for
    TLR2 and TLR4 expression and signaling. Each experiment was run with
    1 to 2 type 2 DM patients, simultaneously with 1 to 2 age-matched
    normal adults as controls. 31 type 2 DM patients and 37 normal
    age-matched controls completed the study. Results showed that blood
    monocytes from type 2 DM patients had a significantly higher TLR4 but
    not TLR2 expression. Using a TLR4 ligand, lipopolysaccharide (LPS), to
    trigger TNFα production, a significantly higher TNFα production by
    blood leukocytes from type 2 DM patients than age-matched controls was
    found. The higher TNFα production by blood leukocytes from type 2 DM
    patients was associated with down-regulation of suppressor of cytokine
    signaling 1and 3 (SOCS-1 and SOCS-3) expression. We have further
    postulated that increase of oxidative stress or decrease of
    IFN-α production in type 2 DM patients was related to the alteration of
    TLR-4 response. Correction of SOCS-1 expression by addition of
    antioxidant, superoxide dismutase (SOD), but not IFN-α, significantly
    decreased TNFα production in blood leukocytes from type 2 DM patients.
    This study is the first in the literature to identify an alteration of TLR4
    expression associated with depressed SOCS-1 expression in leukocytes of
    type 2 DM patients. Results from this study highlight a potential pathway
    to improve chronic inflammation of type 2 DM patients via modulation of
    TLR4 expression and SOCS-1 mRNA expression of leukocytes.
    Advisory Committee
  • Rue-Tsuan Liu - chair
  • none - co-chair
  • Kuender-D Yang - advisor
  • Jiin-Tsuey Cheng - advisor
  • Files
  • etd-0622106-154652.pdf
  • indicate accessible in a year
    Date of Submission 2006-06-22

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have more questions or technical problems, please contact eThesys