工業與環境中有毒之鎘離子(Cd2+)為腎細胞的毒性物質。病變的腎臟
常伴隨著鈣沈積，腎病常引發不正常之腎小管鈣傳訊。本研究是以fura-2
為鈣離子指示劑，探討氯化鎘(CdCl2)對狗腎細胞(Madin Darby canine
kidney cells)內鈣離子濃度([Ca2+]i)變化之影響。當Cd2+濃度大於1

Cadmium (Cd2+) is a widely distributed industrial and environmental toxin , the
effect of Cd2+, a known nephrotoxicant, on intracellular free Ca2+ levels ([Ca2+]i) in
Madin Darby canine kidney (MDCK) cells was explored. [Ca2+]i was measured by
using the Ca2+-sensitive dye fura-2. Cd2+ increased [Ca2+]i in a concentrationdependent
manner with an EC50 of 38

目錄
誌謝.....................................................................................1
中文摘要.....................................................................................2
英文摘要.....................................................................................4
目錄.....................................................................................6
圖表目錄.....................................................................................8
專有名詞縮寫.........................................................................10
第一章緒論............................................................................11
第一節研究動機與目的...............................................11
第二節鎘的環境流佈及生理毒性.............................13
一、急性健康危害.....................................................14
二、慢性健康危害.....................................................14
三、鎘存在的形態.....................................................15
第三節鈣與細胞生理...................................................17
一、鈣在細胞中所扮演的角色................................17
二、細胞鈣訊號的作用機轉....................................18
三、鈣與細胞的毒殺和凋亡....................................22
第二章實驗設計.................................................................26
7
一、研究架構...................................................................26
第三章材料與方法...........................................................27
第一節儀器與材料......................................................27
一、實驗儀器.............................................................27
二、實驗藥品.............................................................27
三、實驗用溶液.........................................................27
第二節實驗方法............................................................28
一、細胞內游離鈣離子濃度的測定........................28
二、細胞存活率分析................................................29
三、細胞凋亡之測定……….………………………29
第三節統計分析..........................................................30
第四章結果............................................................................31
一、鎘離子所誘發之鈣離子傳訊作用....................................31
二、外鈣移除時鎘離子所誘發之[Ca2+]i 升高........................31
三、鎘離子誘發細胞內鈣蓄池釋放之機制............................32
四、PLC 對Cd2+所誘發[Ca2+]i 上升作用之影響....................32
五、PKC 在Cd2+所引起之[Ca2+]i 上升現象中的角色...........32
六、鎘離子對於腎小管細胞的毒殺作用................................33
第五章討論............................................................................48
第六章結論............................................................................51
參考文獻.....................................................................................52
8
圖表目錄
Fig.1 Generation of intracellular Ca2+ signals...........................23
Fig.2 A,family tree

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