在這研究中，我們已經發展一套高性能的多光子激發顯微
系統並成功地擷取KTP晶體與牙齒的二次倍頻影像。本系
統的高靈敏度讓我們可得到512×512 pixels的解析度和
300 seconds/frame 的速度。而從KTP晶體與牙齒表面產
生的二次倍頻訊號也仔細地透過顯微光譜、極化旋轉和波
長調整等方式來檢驗其來源。利用這些方法，我們可以保
證訊號的真實性。KTP晶體與覆蓋在牙質上的琺瑯質是已
知具有高度次序的結構，其各向異性充分顯示在它們的顯
微二次倍頻影像上。

In this study, we have developed a high performance
multi-photon microscopic system to perform second-
harmonic (SH) imaging on a tooth and a KTP crystal
. The high sensitivity of the system allows
acquisition rate of 300 seconds/frame with
resolution at 512×512 pixels. The surface SH
signal generated from the tooth and the KTP
crystal is also carefully verified through
micro-spectroscopy, polarization rotation and
wavelength tuning. In this way, we can ensure the
authenticity of the signal. KTP crystal and the
enamel that encapsulates the dentine is known to
possess highly ordered structures. The anisotropy
of the structure is revealed in the microscopic
SH images of the tooth and the KTP crystal samples.

中文摘要
英文摘要
目錄
圖目錄
表目錄
第一章：導論 1
第二章：共焦掃描顯微原理與簡介 4
2-1單光子共焦掃描顯微原理 4
2-2雙光子共焦掃描顯微激發的原理 10
2-3雷射和激發波長之選擇 12
2-4單光子和雙光子共焦掃描顯微鏡之比較 13
2-5雙光子共焦掃描顯微鏡成像的特點 17
第三章：非線性共焦掃描顯微原理與簡介 18
3-1二次倍頻產生原理 19
3-2偏振原理 23
3-3實驗系統之架設 30
3-3.1雷射光源 31
3-3.2影像掃描系統 32
3-3.3偵測系統 33
第四章：實驗結果及分析 37
4-1實驗目的 37
4-2樣品簡介 38
4-2.1非線性光學晶體-KTP 38
4-2.2牙齒 39
4-3實驗方法 40
4-4觀測結果及分析 43
4-4.1由非線性光學顯微光譜儀得到的結果 43
4-4.2由非線性光學掃描顯微鏡得到的結果 49
第五章：結論及未來展望 60

參考書目 61
附錄A 實驗用各式濾光片之光譜 64

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