大白鼠的動脈壓頻譜訊號可以大致為四個的頻區，即極高頻區（VHF: 5.0-9.0 Hz）、高頻區（HF: 0.8-2.4 Hz）、低頻區（LF: 0.08-0.15 Hz）與極低頻區（VLF: 0.00-0.08 Hz）。而其中低頻區與極低頻區可反應延腦網狀外側核（NRVL）經交感神經對血管壁運動的調控作用。

大白鼠經由靜脈注射內毒素（lipopolysaccharide, LPS; 15 mg/Kg i.v.）而產生急性敗血性低血壓。由頻譜分析動脈壓訊號中的低頻區與極低頻區可以約略的分為三期，第一期：動脈壓訊號中的低頻區與極低頻區的頻譜功率密度有被壓低的現象，第二期：動脈壓訊號中的低頻區與極低頻區的頻譜功率密度有增高的趨勢，而在第三期：動脈壓訊號中的低頻區與極低頻區的頻譜功率密度再次的被壓平，甚至趨近於零。

在NRVL經不同的藥物前處理aCSF、MK-801或CNQX，可以發現MK-801或CNQX減少大白鼠因為敗血性低血壓所產生的死亡率。而高濃度的MK-801（200 pmol）與高濃度的CNQX（10 pmol）維持心跳、血壓在一平穩的狀態，並減緩血壓下降的斜率。而由動脈壓頻譜分析的結果來看，在NRVL中給予高濃度高濃度的MK-801（200 pmol）與高濃度的CNQX（10 pmol）低頻中第二期的功率密度值明顯的較NRVL中經aCSF前處理來的低。

綜合實驗所得得結果，NRVL中的NMDA receptor與non-NMDA receptor在調控由大腸桿菌內毒素所引發敗血症中不可逆轉的低血壓，導致頻譜分析中NRVL的活化時間減短。而NMDA receptor與non-NMDA receptor會的活化會導致血壓急速下降，產生敗血性低血壓而導致大白鼠的死亡，並反應在LF的頻譜功率密度上。

The components of SAP signals of low- frequency (LF: 0.08-0.15 Hz) and very low- frequency (VLF: 0.00-0.08 Hz) related with vasomotor tone that reflects the activity of sympathetic premotor neuron in the nucleus reticularis ventrolateralis (NRVL). The Sprague-Dawely male rats with acute endotoxemia (lipopolysaccharide, LPS; 15 mg/Kg i.v.) induced a reduction phase (phaseⅠ), followed by partial recovery (phaseⅡ) and a secondary decrease (phase Ⅲ). The rats with acute endotoxemia display three phases based on change in the power density of LF and VLF component.

Pretreatment with microinjection of NMDA receptor antagonist, MK-801, and non-NMDA receptor antagonist, CNQX into the bilaterial NRVL prolong the survival time and prolong the duration time of phaseⅡ and phaseⅢ. Pretreatment with high concentration MK-801 (200 pmol) and CNQX (10 pmol) hold the MSAP and heart rate in the steady state and decrease the slope of MSAP falling during endotoxemia.The power density of pretreatment with high concentration MK-801 (200 pmol) and CNQX (10 pmol) was deceease.

We conclude that the rat during experimental endotoxemia decrease the duration time of NMDA and non-NMDA receptor activity in NRVL. The NMDA receptor and non-NMDA receptor activity in NRVL during endotoxemia contribute the slope of MSAP falling and cause to death.

誌謝……………………………1
中文摘要………………………4
英文摘要………………………6
緒論……………………………8
材料與方法……………………21
結果……………………………27
討論……………………………32
結論……………………………40
參考文獻………………………42
圖表……………………………53

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