宿主感染廣東住血線蟲後，IgE抗體會有顯著的增加，本實驗即探討不同部位體液中IgE抗體對不同品系小鼠反應的影響。
本研究選取對廣東住血線蟲有不同抵抗力之四個品系小鼠，其中BALB/c小鼠對廣東住血線蟲的感受性最高，其次為CBA小鼠，C57BL/6及C57BL/10小鼠則用以代表低感受性品系。分析各品系小鼠不同體液中對第五期幼蟲蟲體抗原與排泌抗原的特異性抗體，可知血清與腦脊髓液中的抗體反應與小鼠對腦中蟲體的清除有關，且以IgE最為重要，在腹水中則沒有影響。感受性較高的BALB/c小鼠在腦中IgE抗體增加的程度，較其他品系小鼠低，顯示腦脊髓液中的IgE對蟲體的作用可能較血清中重要。利用西方墨點法(Western blotting)分析腦脊髓液中抗第五期幼蟲抗原的IgE及IgG抗體作用，C57BL/10小鼠在第三週出現的IgE抗體中有一明顯的抗105 kDa蛋白質分子的抗體，此分子在BALB/c小鼠要到第五週才在抗蟲體抗原IgE中出現。在此二品系小鼠抗蟲體抗原的IgG分析中，抗此蛋白質的抗體也分別在相同時間出現，推測在腦脊髓液中的105 kDa抗原分子可能對引起廣東住血線蟲保護性的抗體免疫反應有相當的關連性，此蛋白質分子的作用及特性分析值得進一步研究。

The IgE response increased in hosts infected with Angiostrongylus cantonensis. This study analyzes IgE responses appeared in different body fluids of mice infected with A. cantonensis.
BALB/c, CBA, C57BL/6 and C57BL/10 strain of mice were used in the experiment. BALB/c mice exhibited the highest susceptibility to A. cantonensis, CBA mice showed intermediate susceptibility, while C57BL/6 and C57BL/10 mice showed the lowest susceptibility. Analyses of specific antibodies against L5 antigens, and ES antigens in mice showed antibodies appeared in sera and in cerebrospinal fluids(CSF), especially IgE antibodies, were related to the worm rejection happened in mouse brain. However, antibodies appeared in ascites had no influence. The level of IgE increased in brain of susceptible BALB/c mice is much lower than that increased in other strain. Our data suggested that IgE response elicited in brain might be much more important than that in serum. Western blot analyses showed that among antigens extracted from L5 both IgE and IgG responses were positive in CSF. IgE antibodies against a 105 kDa protein of L5 antigen in C57BL/10 mice appeared in third week after infection, while in BALB/c mice appeared in fifth week. IgG antibodies against 105 kDa protein appeared in the identical weeks.
Our results indicated that 105 kDa protein was related to specific antibodies responses about protective function of mice infected with A. cantonensis. Further researches will be required to understand function and characterization of this protein.

摘要………………………………………………………………Ⅰ
英文摘要…………………………………………………………Ⅱ
目錄………………………………………………………………Ⅲ
圖表目錄…………………………………………………………Ⅳ
前言………………………………………………………………1
材料與方法………………………………………………………6
結果………………………………………………………………14
討論………………………………………………………………24
參考文獻…………………………………………………………30
圖表………………………………………………………………35

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