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論文名稱 Title |
夜來香花芽分化前、中、後cytokinins及gibberellins含量變化之研究 Changes in Cytokinin and Gibberellin Levels Before, During and After Floral Initiation in Polianthes tuberosa |
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系所名稱 Department |
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畢業學年期 Year, semester |
語文別 Language |
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學位類別 Degree |
頁數 Number of pages |
50 |
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研究生 Author |
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指導教授 Advisor |
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召集委員 Convenor |
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口試委員 Advisory Committee |
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口試日期 Date of Exam |
2000-06-18 |
繳交日期 Date of Submission |
2000-06-28 |
關鍵字 Keywords |
細胞分裂素、開花、激勃素、夜來香 gibberellins, cytokinins, Polianthes tuberosa, flowering |
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統計 Statistics |
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中文摘要 |
本論文以研究外加與內生之細胞分裂素(cytokinins)和激勃素(gibberellins)對夜來香花芽分化過程的影響 為主要目的。在cytokinins的分析方面,以研究分析夜來香球莖在營養生長、花芽分化初期及花芽發育各期之 內生cytokinins的含量變化,另外也探討外加cytokinins處理對夜來香花芽分化及發育的相關性。結果發現外加 cytokinins有效地誘導花芽分化及其發育,尤其在花芽分化初期與花芽發育期似乎有比較明顯效用。且內生 cytokinins的含量在花芽分化初期與花芽發育期也相對較營養生長期高。在夜來香花芽分化期間,zeatin和 dihydrozeatin的含量顯著的增加,而zeatin riboside、dihydrozeatin riboside、6N-(δ2-isopentenyl) adenine和6N-(δ2-isopen -tenyl) adenine riboside則沒有顯著的變動,且含量較低。所以,花芽誘導期間 cytokinins含量的增加可能對夜來香之花的呼喚(apex evocation)扮演一個重要角色。而且,這些結果也說 明cytokinins對誘導夜來香開花及花芽發育有促進作用。 在gibberellins的分析方面,夜來香球莖之內生gibberellins經由高效能液相層析儀(HPLC)分離和生物 檢定(bioassay),最後用氣相層析質譜儀(GC-MS)分析定性。實驗結果發現,夜來香在營養生長、花芽分化初 期和花芽發育各期之內生gibberellins主要有gibberellin A19、gibberellin A20及gibberellin A53。此項結果顯示13-hydroxylated gibberellins存在於夜來香球莖組織中。此外,當夜來香從營養生長階段轉 變到花芽分化初期和花芽發育期時,也伴隨發生gibberellin A20之增加和gibberellin A19之減少。而 gibberellin A53含量在三個不同生長時期則維持一定。另外,當以gibberellin A3、gibberellin A4、 gibberellin A20和gibberellin A32處理營養生長時期的夜來香球莖(植株約5公分高)時,實驗結果發現 gibberellin A3、gibberellin A20和gibberellin A32能誘導與增進其花芽分化。說明hydroxylated C-19 gibberellins在夜來香之開花誘導作用上扮演重要角色。 |
Abstract |
We studied how the endogenous and exogenous cytokinin and gibberellin functions in floral initiation and development in tuberose. In the aspect of cytokinins. The contents of endogenous cytokinin in tuberose corms (Polianthes tuberosa L. cv. Double) at vegetative, early floral initiation, and flower development stages were investigated. We also determined the influence of exogenous cytokinin treatment on the corm apex at three different growth stages in relation to floral initiation and development in tuberose. The exogenous cytokinin effectively induced floral initiation and development, especially at the early floral initiation and flower development stages. Endogenous cytokinins were higher in early floral initiation and development stages in comparison to the vegetative stage. During floral initiation stage, the zeatin and dihydrozeatin increased significantly, while the cytokinins, zeatin riboside, dihydrozeatin riboside, 6N-(δ2-isopentenyl) adenine, and 6N-(δ2-isope -ntenyl) adenine riboside at consistently low levels. The increase of cytokinin levels in tuberose corms during floral induction suggests a role for cytokinins in tuberose apex evocation. Moreover, these results indicate that cytokinins seem to promote the development of flower buds rather than inducing flowering in tuberose. Endogenous gibberellins (GAs) in tuberose corms were isolated using high performance liquid chromatography, bioassay and identified by combined capillary gas chromatography-mass spectrumetry. Gibber -ellins A19, A20 and A53 were quantified at the vegetative, early floral initiation and flower differentiation stages. The identification of these 13-hydroxylated GAs indicates the presence of the early-13-hydroxy -lation in tuberose corms. An increase in GA20 and decrease in GA19 coincided with the transition from the vegetative phase to the stages of early floral initiation and flower differentiation. GA53 maintained at constant levels at three different growth stages. When GA3, GA4, GA20 and GA32 were applied to corms at vegetative stage (plants about 5 cm in height ), floral initiation was induced and/or promoted by several, most notably by GA3, GA20 and GA32. It is suggest that hydroxylated C-19 GAs play an important role in flower induction in tuberose. |
目次 Table of Contents |
碩士論文……………………………………………I 中文摘要……………………………………………II 英文摘要……………………………………………IV 目錄…………………………………………………VI 圖表目錄……………………………………………VII 前言…………………………………………………1 Cytokinins含量變化之分析………………………4 材料與方法…………………………………………4 植物材料………………………………………… 4 定性分析………………………………………… 5 定量分析………………………………………… 9 結果與討論……………………………………… 10 Gibberellins含量變化之分析………………… 15 材料與方法……………………………………… 15 植物材料…………………………………………15 定性分析…………………………………………16 定量分析…………………………………………19 結果與討論……………………………………… 21 參考文獻………………………………………… 26 圖表……………………………………………… 33 |
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