本研究之目的在探討馬拉巴石斑稚魚之最適鋅需求。實驗飼料以蛋清蛋白粉與土魠鰆肌肉粉提供52%之粗蛋白。鋅含量為10.0 mg/kg的基本飼料，分別添加0、4、8、12、24、36、140 mg/kg的硫酸鋅配製成實驗飼料。馬拉巴石斑稚魚初重5.9公克，每缸十二尾，每飼料處理組各有三重複，每日餵食量約魚體濕重的4％，經十八週的飼育，飼料中之鋅含量顯著影響馬拉巴石斑稚魚的成長，無添加鋅組之增重率及飼料效率顯著低下。而鋅添加對活存率、肥滿度、肌肉組成、脊椎與鱗片灰份含量、各血液學分析皆無顯著影響。飼料鋅添加對馬拉巴石斑魚血清、肌肉、脊椎、鱗片之鋅含量造成顯著影響。就非專一性免疫為指標的研究發現：飼料鋅添加對巨噬細胞的吞噬力並無顯著影響，同樣的替代路徑補體活性、凝集力價與溶菌酵素活性皆無顯著變化。同樣的飼料鋅添加對含銅、鋅之金屬酵素，超氧歧化酵素（SOD）活性亦無造成顯著影響。將增重率、飼料效率、血清鋅含量、脊椎鋅含量與鱗片鋅含量等參數以Broken-line模式推估馬拉巴石斑稚魚鋅需求量分別為33.7、35.6、32.5、28.9及32.6 mg Zn /kg，因此為了維持馬拉巴石斑稚魚之適當成長與組織正常含鋅量，飼料最適鋅含量介於28-36 mg/kg之間。

The purpose of the present study was to quantify the optimal zinc requirements of juvenile grouper (Epinephelus malabaricus). Basal diet contained 52 % crude protein from derived egg white and mackerel（Scomberomorus commerson）muscle. This semipurified basal diet which contained 10.0 mg Zn/kg was supplemented with ZnSO4 at levels of 0, 4, 8, 12, 24, 36 or 140 mg Zn/kg, respectively. The test diets were fed to juvenile groupers with an initial weight of about 5.9 g. All treatments were triplicated, each tank had 12 fish. After 18 weeks of rearing, dietary zinc levels have significant effects on the growth of fish. Weight gain and feed efficiency were significantly lower in grouper fed the unsupplemental diet than the supplemental groups. No significant difference among fish groups was found in survival rate, condition factor, muscle composition, ash contents of vertebra and scale, as well as some hematological parameters. Zinc concentrations in serum, muscle, vertebra and scale were affected by the supplemental zinc level. There was no significant difference in nonspecific immunity parameters including phagocytosis of macrophage, alternative complement pathway (ACH50) activity, agglutination titer and lysozyme activity among dietary treatments. The activity of the copper and zinc metalloenzyme superoxide dismutas in red blood cells was also not affected by dietary zinc intake. Broken-line regression analysis of weight-gain, feed efficiency as well as serum-, vertebra- and scale zinc concentrations against dietary zinc intake indicated requirement values of 33.7, 35.6, 32.5, 28.9 and 32.6 mg Zn/kg diet, respectively. Therefore, based on the results of growth performance and tissue zinc concentrations, the dietary zinc requirement of the juvenile groupers was between 28-36 mg Zn/kg diet.

中文摘要
英文摘要
謝辭
文獻回顧
前言
材料方法
結果
討論
參考文獻
履歷表

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