厚殼桂科(Cryptocarya)，其天然衍生物有許多生物活性被發表，像是對某些癌症的抗增生作用。然而，厚殼桂天然衍生物卻很少用在口腔癌方面。本論文使用厚殼桂科植物根部的甲醇粗萃物(MECCrt)，評估對口腔癌的可能抗增生作用。我們發現MECCrt對於此次實驗所使用的兩株口腔癌細胞Ca9-22和CAL 27，依著藥物劑量在細胞存活率上有顯著性的抑制(p < 0.01)。流式細胞分析儀實驗顯示Ca9-22和CAL 27兩株細胞都出現subG1比例增加，並產生細胞凋亡。深入探討發現在MECCrt處理下細胞內reactive oxygen species (ROS)含量，隨著處理劑量和處理時間增加而增加(p < 0.01)。MECCrt也同時顯著性的降低兩株細胞株的粒線體膜電位 (p < 0.01–0.05)。以結論是：MECCrt具有抗口腔癌的增生能力(proliferation)，其機制可能係透過細胞凋亡(apoptosis)、ROS增加以及粒線體膜電位(mitochondrial membrane potential)的去極化(depolarization)。

Cryptocarya-derived natural products were reported to have many biological effects such antiproliferation of some cancers. However, the possible antioral cancer effect of Cryptocarya-derived natural products was less addressed. In this study, we firstly the methanol extract of Cryptocarya plant root (MECCrt) to evaluate its potential function of antioral cancer. We found that MECCrt significantly reduced cell viability of two oral cancer Ca9-22 and CAL 27 cells in dose-responsive manners (p < 0.01). For the flow cytometry, the sub-G1 population and annexin V-intensity of MECCrt-treated Ca9-22 and CAL 27 cells significantly accumulated in a dose-responsive manner (p < 0.01). These apoptotic effects were associated with the findings that intracellular ROS generation were induced in MECCrt-treated Ca9-22 and CAL 27 cells in dose-responsive and time-dependent manners (p < 0.01). MECCrt also displayed significant reduction of mitochondrial membrane potential in these two cells in a dose-responsive manner (p < 0.01–0.05). Taken together, these results suggest that MECCrt has an antiproliferative potential against oral cancer cells through apoptosis, ROS induction, and mitochondria membrane depolarization.

論文審定書…………………………………………………………………...i
誌謝…………………………………………………………………………..ii
中文摘要……………………………………………………………..……...iii
英文摘要………………………………………………………………..…...iv
目錄………………………………………………………...…………..…….v
表目錄………………………………...………………………….….………vi
圖目錄………………………………………………………………..……..vii
縮寫表……………………………………………………………………...viii
壹、 介紹………………………………………………………...…………1
貳、 實驗材料與方法…………………………………………...…………3
1. 藥物製備……………………………………………....……………..3
2. 實驗材料來源及訂購方法…………………………....……………..3
3. 細胞培養……………………………………………..….……………6
4. 細胞活性…………………………………………..…………...….…6
5. 細胞週期………………………………………….………...………..7
6. 細胞凋亡分析……………………………………………...…...……9
7. 細胞內活性氧化物…………………….……...……………...…....10
8. 粒線體膜電位……………………………......……………...……..11
參、 實驗結果……………………………………..………….………….13
肆、 討論…………………………………………………..…..…………16
伍、 結論……………………………………………..…..………………17
陸、 圖表…………………………………………………..……………..18
柒、 參考文獻…………………………………………..…….………….41

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