生長素(auxin)能誘導植物發不定根，其中，人工合成的生長激素，引朵丁酸(IBA)，能有效低促進牛樟插穗不定根的誘發。推測IBA藉由抑制過氧化酶(POX)的活性誘導逆分化在不定根發育時形成新的根源母細胞。因此，藉其他物種已發表的序列設計退化性引子，在牛樟幼根中篩選出POX cDNA，並利用5’和3’RACE完成整段的cDNA序列，CKPX1 和 CKPX3。CKPX1 與 CKPX3 推估轉譯的氨基酸序列經比對，與其他已發表的POXs相似度在40%至60%之間。並針對 CKPX 3 的上游調控區進行序列分析，發現與auxin及木質素合成相關調控序列，以及抗病與逆境誘導的調控序列，顯示CKPX3 受到生長激素的調控並參與不定根發育外，也參與植物抗病的機制中。

Auxin can induce adventitious rooting. Synthetic auxin, indole-3-butyric acid (IBA), effectively promoted the rooting in Cinnamomum kanehirae. The peroxidase (POX) activity significantly decreased in the IBA-treated tissues as compared with the control. Hence, I suggest that the inhibition on POX activity may lead to the redifferentiation processes induced by IBA, which produces the new root primordia during the formation of adventitious roots. On this investigation, I cloned POX cDNA from the young roots. Degenerate primers were designed from the conservative regions of other published POX to amplify the expectant DNA fragment. Full-length cDNA of the POX gene designated CKPX1 and CKPX3 were cloned by the method of 5'and 3' RACE. The deduced amino acid of CKPX1 and CKPX3 were compared with the previously reported POX and showed between 40% and 60% identity with those plant species. Further studies on the promoter elements of CKPX3 were found out that elements related to auxin response, lignification, pathogen invasion and stress response. The results suggest that CKPX3 may be involved in the regulate process of adventitious rooting and defense against pathogens and environment stress.

Chinese abstract ------------------------------------- I

English abstract ------------------------------------- II

Table of contents ---------------------------------- III

List of tables and figures ------------------------- IV

Introduction ------------------------------------------ 1

Materials and Methods ----------------------------- 6

Results ---------------------------------------------- 11

Discussion ------------------------------------------ 15

Reference ------------------------------------------- 19

Tables and figures --------------------------------- 24

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