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論文名稱 Title |
澳洲球形海綿內共生細菌多樣性研究 Diversity of Endosymbiotic Bacteria of the Sponge, Cinachyrella australiensis |
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系所名稱 Department |
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畢業學年期 Year, semester |
語文別 Language |
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學位類別 Degree |
頁數 Number of pages |
54 |
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研究生 Author |
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指導教授 Advisor |
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召集委員 Convenor |
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口試委員 Advisory Committee |
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口試日期 Date of Exam |
2012-06-15 |
繳交日期 Date of Submission |
2012-06-30 |
關鍵字 Keywords |
螢光原位雜合、冷凍超薄切片、共生細菌、澳洲球形海綿 cryo-section, Fluorescent in situ hybridization(FISH), Cinachyrella australinesis, bacteria symbiont |
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統計 Statistics |
本論文已被瀏覽 5660 次,被下載 1027 次 The thesis/dissertation has been browsed 5660 times, has been downloaded 1027 times. |
中文摘要 |
海綿為原始的多細胞生物且為重要二次代謝物來源,在許多研究中指出其內包含多樣性共生菌群且此群聚組成狀態相當穩定。但是對於此共生細菌群如何保持穩定的組成狀態及其如何傳遞共生菌群至下一代,目前我們尚未完全明瞭此機制。為增進我們對海綿及其共生菌的瞭解,在此針對澳洲球形海綿利用螢光原位雜合法研究其共生細菌之分佈情形,並使用標定細菌的非專一性探針(EUB338)及文獻中此海綿優勢菌之資料,分別做為此研究標定海綿共生菌探針之設計,利用冷凍超薄切片進行樣本的處理,使樣本在最短的樣本處理過程中進行原位雜合,以期能充分瞭解海綿共生細菌的分佈。雜合影像顯示出多種先前研究所表現的影像,可以確認在澳洲球形海綿中共生細菌的組成相當的穩定。螢光原位雜合探針標示共生菌的位置,顯示此共生細菌為內共生細菌,及以垂直傳遞之方式將共生菌傳至子代。 |
Abstract |
Sponge are primitive multi-cellular organisms. They are important sources of secondary metabolites. In the previous studies indicated that the sponges harbor stable symbiotic microbial consortia. The mechanisms for maintenance and transmission of microbial consortia to the next generations are still not fully understood. The sponge, Cinachyrella australinesis, was chosen to further investigate relationship of the symbiotic bacteria within to the host. Fluorescent in situ hybridization (FISH)was employed with non-specific (EUB338)and specific oligonucleotide probes for bacteria. The sponge was cryo-sectioned(1μm)and hybridized with fluorescent probes. The distribution and ratios of the bacteria in the sponge agreed with those of previous studies indicating that the symbiotic bacteria of C. australiensis are stable and endosymbiotic in nature. |
目次 Table of Contents |
第一章 緒論........................................ 1- 10 第二章 材料與方................................11-14 第三章 實驗結果................................15-18 第四章 討論........................................19-22 第五章 參考文獻.................................23-29 |
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