本研究主要為具有微圓柱之微渠道，在電動驅動流下的流場情形，其微圓柱型式為平形式和交錯式兩種，微圓柱大小為直徑20 μm、30 μm和40 μm三種，最後加入λDNA分子觀察拉伸情形。整個實驗以微質點影像測速儀（Micrometer resolution particle image velocimetry, μPIV）觀測流場速度分布和計算流場應變率，以雷射誘發螢光（Laser induced fluorescence, μLIF）檢測因焦耳熱效應產生之流場溫度分布，以螢光顯微鏡（Fluorescence microscope, FM）觀測DNA分子拉伸影像。實驗中微渠道以光微影製程製出，將經氧化電漿的PDMS渠道上部與底部接合，通入工作流體後，於兩端加入電場驅動，其中工作流體為1×TBE，配置黏度分別為1、20和60 cP之緩衝溶液，電場強度使用5、8、10、15、20和25 kV/m。最後經由實驗得到校正速度、流場流動情形和DNA分子在微圓柱渠道拉伸情形等等。

In this study, electrokinetic-driven flow in microchannels with different types and sizes of micropillars was observed. There were parallel and staggered micropillars in types and 20 μm, 30 μm and 40 μm in diametral sizes. At last stretched λDNA was also observed. We utilized micrometer resolution particle image velocimetry (μPIV) to observe and calculate the field of velocity. And the field of temperature caused joule heating effect was detected by laser induced fluorescence (μLIF). Used fluorescence microscope (FM) recorded the image of DNA molecules. In the experiment, microchannels were produced by photo lithogrophy process which composed of two slices of PDMS after UV/Ozone. And different viscosities and intensity of electric field in 1 x TBE buffer solution were performed to gain corrected velocity, flow field, stretched DNA in microchannels with micropillars and so on.

目　錄 i
表 目 錄 iii
圖 目 錄 iv
符號說明 vi
中文摘要 ix
Abstract x
第一章　序論 1
1-1 前言 1
1-2 DNA分子結構與性質 1
1-3 微機電系統與生醫晶片 3
1-4 背景與目的 4
1-5 文獻回顧 7
第二章 實驗系統與設備 15
2-1 μPIV及μLIF系統 15
2-2 全內反射顯微鏡 16
2-3 製程設備 17
2-4 其他實驗週邊設備 19
第三章　實驗方法及步驟 24
3-1 微流道設計與製程 24
3-2 工作流體配製 27
3-3 μPIV、μLIF和FM量測系統建立及原理 27
3-4 實驗量測參數 30
第四章　理論分析 45
4-1 校正速度理論 45
4-2 DNA分子流變學拉伸理論 46
4-3 無因次參數分析 47
第五章　誤差分析 51
第六章　結果與討論 55
6-1 校正速度量測 56
6-2 不同y位置速度量測 59
6-3 流場向量分析 62
6-4 DNA分子螢光可視化分析 64
6-5 DNA無因次參數分析 70
第七章　結論與建議 96
7-1 結論 96
7-2 建議與改進 97
參考文獻 98
附錄 A 109

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