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博碩士論文 etd-0702102-160624 詳細資訊
Title page for etd-0702102-160624
論文名稱
Title
綠島產肥厚肉質與蜿蜒繖軟珊瑚化學成分及癌細胞毒殺活性之研究
Chemical Constituents and Cytotoxicity of Formosan soft Corals Sarcophyton crassocaule an Xenia puerto-galerae
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
138
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2002-05-29
繳交日期
Date of Submission
2002-07-02
關鍵字
Keywords
癌症、珊瑚
Xenia puerto-galerae, soft Corals, Sarcophyton crassocaule, cancer
統計
Statistics
本論文已被瀏覽 5682 次,被下載 1969
The thesis/dissertation has been browsed 5682 times, has been downloaded 1969 times.
中文摘要
綠島產肥厚肉質(Sarcophyton crassocule Von. Marenzeller)軟珊瑚之有機層萃取物經細胞活性測試,發現其對P-388、HT-29及A-549三種癌細胞有毒殺活性,以癌細胞毒殺活性為指標,再將二氯甲烷之萃取物以矽膠管柱層析進行分離純化後,得到四個cembrane diterpenoid化合物,分別是GN16-34 (1), GN16-35 (2), GN16-40 (3), GN16-62 (4),其中化合物4是新的新化合物。
綠島產蜿蜒繖(Xenia puerto-galerae Roxas)軟珊瑚之二氯甲烷層及丙酮層粗萃取物,以矽膠管柱層析及高效能液相色層分析儀(high-performance liquid chromatography, HPLC)進行分離純化後,得到六個cadinane sesquiterpenes化合物,分別為GN44-28 (5)、GN44-30 (6)、GN44-44 (7)、GN44-66 (8)、GN44-173 (9)、GN44-149 (10)及一個diterpenes,為GN44-199 (11),其中化合物5-10是新的合物。
上述化合物經癌細胞毒殺活性測試後,化合物3及4對P-388、HT-29及A-549細胞株均有顯著的抑制作用,化合物1及2對P-388細胞株有抑制作用,化合物5及7對A-549細胞株有顯著的抑制作用,化合物10對P-388及A-549細胞株均有抑制作用。

Abstract
The methylene chloride extracts of Formosan soft coral Sarcophyton crassocule Von. Marenzeller (collected at Green Island off Taiwan) were found to exhibit significant cytotoxicity against P-388, HT-29, and A-549 cancer lines. Chromatographic separation led to the isolation of three known cembrane diterpenoids, GN16-34 (1), GN16-35 (2), GN16-40 (3), and a new cembrane, GN16-62 (4).
The methylene chloride and acetone extracts of Formosan soft coral Xenia puerto-galerae Roxas (collected at Green Island off Taiwan) were found to exhibit significant cytotoxicity against P-388 and A-549 cancer cell lines. Chromatographic separation resulted in the isolation of six new cadinane sesquiterpenes, GN44-28 (5), GN44-30 (6), GN44-44 (7), GN44-66 (8), GN44-173 (9), GN44-149 (10), and one known dicarbocyclic diterpenes with a bicyclic [4.3.1] ring system, GN44-199 (11).
Compounds 3 and 4 exhibited significant cytotoxicity against P-388, HT-29, and A-549 cancer cell lines. Compounds 1 and 2 exhibited significant cytotoxicity against P-388 cancer cell lines. Compounds 5 and 7 exhibited significant cytotoxicity against A-549 cancer cell line. compound 10 exhibited significant cytotoxicity against P-388 and A-549 cancer cell lines.

目次 Table of Contents
壹、 緒論
一、 前言
二、 Sarcophyton屬軟珊瑚之天然物化學研究回顧
三、 Xenia 屬軟珊瑚之天然物化學研究回顧
四、 研究動機及目的
貳、 肥厚肉質軟珊瑚化學成分之研究
一、 材料採集與鑑定
二、 肥厚肉質軟珊瑚之分類地位
三、 肥厚肉質軟珊瑚之萃取與分離
四、 肥厚肉質軟珊瑚化學成分之分離純化
(1) GN16-34 (1)之分離純化
(2) GN16-35 (2)之分離純化
(3) GN16-40 (3)之分離純化
(4) GN16-62 (4)之分離純化
五、 肥厚肉質軟珊瑚化學成分之結構解析
(1) GN16-34 (1)之結構解析
(2) GN16-35 (2)之結構解析
(3) GN16-40 (3)之結構解析
(4) GN16-62 (4)之結構解析
參、 蜿蜒繖軟珊瑚化學成分之研究
一、 材料採集與鑑定
二、 蜿蜒繖軟珊瑚之分類地位
三、 蜿蜒繖軟珊瑚之萃取與分離
四、 蜿蜒繖軟珊瑚化學成分之分離純化
(1) GN44-28 (5)之分離純化
(2) GN44-30 (6)之分離純化
(3) GN44-44 (7)之分離純化
(4) GN44-66 (8)之分離純化
(5) GN44-173 (9)之分離純化
(6) GN44-149 (10)之分離純化
(7) GN44-199 (11)之分離純化
五、 蜿蜒繖軟珊瑚化學成分之結構解析
(1) GN44-28 (5)之解構解析
(2) GN44-30 (6)之解構解析
(3) GN44-44 (7)之解構解析
(4) GN44-66 (8)之解構解析
(5) GN44-173 (9)之解構解析
(6) GN44-149 (10)之解構解析
(7) GN44-199 (11)之解構解析
肆、 化合物之細胞毒殺活性
伍、 結論
陸、 參考文獻
柒、 附錄
一、 儀器及材料
二、 癌細胞毒殺活性測試之分析方法
三、 MTT分析原理
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