於本論文中我們比較了單光子及雙光子激發對阿拉伯芥( Arabidopsis thaliana )原生質體的影響，並且可利用高空間解析度之時基顯微光譜來研究阿拉伯芥原生質體內葉綠體在雷射激發下的反應。我們發現在脈衝雷射所造成之多光子激發下，葉綠體的螢光光譜顯示出戲劇性的變化，而且原生質體被迅速地破壞。相較之下，利用連續雷射作單光子激發時，其原生質體之生命力並沒有受到影響。除此之外，我們亦利用低溫顯微光譜對PPV作量測，在低溫下發現PPV之螢光將會產生紅位移之傾向，而且非輻射性的能量轉移大幅降低，使其螢光強度大幅增強。

In this thesis the effects of single photon and multi-photon excitation on protoplasts from Arabidopsis thaliana are compared. Time-lapsed micro-spectroscopy at high spatial resolution is employed to study the response of chloroplasts within the protoplasts from Arabidopsis thaliana. We have found that the fluorescence spectra of chloroplasts exhibits dramatic changes and the protoplasts are rapidly damaged under multi-photon excitation as a result of pulsed laser illumination. In contrast, single photon excitation of chloroplasts with cw laser is relatively inert to the vitality of the protoplasts. In addition to, we have built an ultrafast laser excited cryogenic micro-spectroscopy setup to study the photoluminescence of PPV thin film. We found that the spectrum of PPV’s photoluminescence should shift toward longer wavelength and the non-radiative transition should be suppressed as a result of longer electron coherence length at low temperature.

中文摘要
英文摘要

第一章 導論 8

第二章 實驗樣品介紹 15
2-1 葉片及葉綠體之結構 16
2-2 葉綠體的吸收光譜與作用光譜 18
2-3 阿拉伯芥 20
2-4 PPV薄膜 20

第三章 顯微光譜系統 22
3-1 實驗目的 22
3-2 實驗系統之介紹與架設 23
3-3 實驗方法 32
3-4 實驗結果與分析 33

第四章 低溫顯微光譜系統 41
4-1 實驗目的 41
4-2 實驗系統之架設 41
4-3 實驗方法 42
4-4 實驗結果與分析 43

第五章 結論與未來展望 45

附錄一 共焦掃描顯微鏡成像之原理 47
6-1 薄透鏡之繞射 47
6-2 三維同調影像的形成 56

附錄二 顯微光譜程式 63

參考文獻 65

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