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博碩士論文 etd-0704107-102932 詳細資訊
Title page for etd-0704107-102932
論文名稱
Title
黃鰭鯛卵細胞成熟之研究
The Studies of Oocyte Maturation in Protandrous Yellowfin Porgy, Acanthopagrus latus
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
131
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2007-05-29
繳交日期
Date of Submission
2007-07-04
關鍵字
Keywords
最後成熟激素、黃鰭鯛、激素
maturation inducing steroid, MIS, steroids, Yellowfin porgy
統計
Statistics
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中文摘要
本研究的目的為瞭解黄鰭鯛生殖腺發育、成熟,體內性激素特性,並探討黃鰭鯛卵細胞最後成熟激素之種類,探究其基礎生殖內分泌調控機制,以供後續研發與應用之依據。
一齡魚為具有產精能力之功能性雄魚 (Functional males),同時存在之卵巢組織佔全生殖腺之比例很小,以卵原細胞和卵母細胞為主,緩慢發育。二齡魚開始有性轉變發生,約有30~50 %可自然性轉變成雌魚,會性轉變成雌魚者,卵細胞在八月份開始有卵黄生成,GSI值於十一月份繁殖盛期達6.88 %最高值。三齡黃鰭鯛大約則有80 %可自然性轉變成雌魚,GSI值最高可達14.35%,明顯高於初次成熟之二齡魚,卵細胞發育過程和二齡雌魚相同。
血液中T含量,一齡魚、二齡雄魚在九~十二月較高,而於繁殖季節的十一月達最高量, 二、三齡雌魚於繁殖季節含量很低,雌魚於卵細胞最後成熟時無顯著性差異變化。血液中E2含量,一齡魚、二齡雄魚在各月份均很低, 二、三齡雌魚在九~十二月較高,而於繁殖季節的十ㄧ月達最高量,雌魚於卵細胞最後成熟時有顯著性之增多。
黃鰭鯛在最後成熟階段GSI值增至為催熟前之1.5倍,可作為判斷卵細胞是否進入最後成熟階段之依據;GSI值增至為催熟前之2倍時期,便表示卵細胞發育至GVBD階段已完成最後成熟。
在卵細胞發育成熟過程,依據外觀形態之變化,可區分為 (ㄧ) 卵黃生成前發育期:二至七月,卵群分佈以卵徑15~25 mm 之卵原細胞及100 mm 之初級卵母細胞為主。(二) 卵黃生成期:八~九月為內卵黃生成期,十月為外卵黃生成期,卵徑在卵黃蓄積完成時為400
Abstract
The objectives of this study were to investigate the characteristics and mechanism of gonad maturation including identified maturation inducing steroids and its physiological role in the final oocyte maturation (FOM) in yellowfin porgy.
yellowfin porgy, Acanthopagrus latus are functional male for the first year of life but begin to change sex during the second year. Only 30~50% of cultured yellowfin porgy are change into females in 2-year-old, while 80% fish become females at 3-year-old. Vitellogenesis were observe between August and November and have the highest gonadosomatic index (GSI %) at 6.88 % and 14.35 % in 2 and 3-year-old female yellowfin porgy respectively.
Highest serum levels of T occurred simultaneously during spawning season in one and two years of age in functional male, while highest level concentration E2 were observed at the same time in two and three years old female fish.
GSI increased 1.5-fold during FOM and reach 2-fold at germinal vesicle breakdown stage (GVBD).
Oogenesis in 3-year-old female yellowfin porgy was divided into three stages;(1) previtellogenic stage from February to July with the primary oocytes;(2) vitellogenic stage from August to October and with full growth oocyte of diameter at 400
目次 Table of Contents
章 次 頁 次
中文摘要………………………………………………………………………………… Ⅱ
英文摘要………………………………………………………………………………… Ⅳ
目 錄………………………………………………………………………………… Ⅵ
圖表目錄………………………………………………………………………………… Ⅶ
縮 寫 表………………………………………………………………………………… Ⅸ
研究架構………………………………………………………………………………… XI
緒 論 ………………………………………………………………………………… 1
材料和方法……………………………………………………………………………… 14
結 果………………………………………………………………………………… 27
討 論………………………………………………………………………………… 39
結 論………………………………………………………………………………… 59
參考文獻………………………………………………………………………………… 61
圖………………………………………………………………………………………… 77
參考文獻 References
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