蟾魚的發音肌是目前脊椎動物中收縮最快速的肌肉，並且肌肉細胞中鈣離子傳遞的速度和橫橋分離的速度也是脊椎動物中最快的。小清蛋白已知為肌肉細胞中之鈣結合蛋白，可幫助肌肉的放鬆。目前已知的小清蛋白具有數種等形，且在不同的肌肉細胞(例如:紅肌、白肌、粉紅肌)中，其表現量各異。印尼奇蟾魚(Allenbatrachus grunniens)的雌雄個體在魚鰾上皆有內生型發音肌。本論文比較雌雄個體發音肌形態，結果發現在長度、寬度、厚度及重量上，雌雄發音肌皆無顯著差異。再利用SDS-PAGE與西方墨點法分離及鑑定發音肌與體側肌中的小清蛋白，比較其總表現量，結果無顯著差異，顯示小清蛋白含量與肌肉放鬆速度並無一定關聯。進一步使用原態聚丙烯醯胺膠體電泳(Native-PAGE)分析印尼奇蟾
魚發音肌與體側肌中的小清蛋白等形，分別鑑定出2 種與4 種等形，其中小清蛋白等形1、小清蛋白等形2 及小清蛋白等形3 的分子量分別為10kDa、10.5kDa及10.5kDa，等電點為4.77、4.58及4.42。發音肌中主要為小清蛋白等形1 (Parv1)，佔總小清蛋白的94%以上，其次為小清蛋白等形2 僅佔5%；而體側肌中主要為小清蛋白等形2，佔總小清蛋白的58%，其次為小清蛋白等形1(及小清蛋白等形1a)與小清蛋白等形3 各佔約20%。小清蛋白等形1 對印尼奇蟾魚發音肌的快速放鬆影響可能最大。

The sonic muscle of toadfish is the fastest vertebrate muscle ever measured, and
the rate of transport of Ca2+ and dissociation of cross-bridge are also fatest.
Parvalbumins are Ca2+-binding proteins present in vertebrate muscle, and they can aid
muscle relaxation. Several isoforms of parvalbumin had been identified and presented
in variable proportion in different kinds of muscles (e.g. red muscle, white muscle and
pink muscle). Both male and female grunting toadfish (Allenbatrachus grunniens)
have intrinsic sonic muscles attached on swim bladders. The morphology of male and
female sonic muscle was compared, and no significant differences in both length,
width, thickness and weight were found. SDS-PAGE and western blotting were used
to determine the total parvalbumin expression and identify the parvalbumins from
sonic muscle and body white muscle. There were no significant differences in total
parvalbumin expression in sonic muscle and body white muscle. The result indicates
that there is no positive correlation between high content of parvalbumins and speed
of muscle relaxation. In native-PAGE, two and four parvalbumin isoforms were
identified from sonic muscle and body white muscle, respectively. The estimated size
of Parv1, Parv2 and Parv3 size in grunting toadfish’s sonic muscle were 10kDa,
10.5kDa and 10.5kDa, respectively, and the isoelectric points of Parv1, Parv2 and
Parv3 in grunting toadfish were 4.77, 4.58 and 4.42, respectively. In the sonic muscle,
the major parvalbumin isoform was parvalbumin isoform 1 (Parv1), which comprised
more than 94% of total parvalbumin, and parvalbumin isoform 2 (Pav2) comprised
vi
only 5% of total parvalbumin content. In body white muscle, on the other hand, the
major isoform was parvalbumin isoform 2 (Parv2) which comprised 58% of toal
parvalbumin. Both Parv1 (with Parv1a) and Parv3 comprised about 20%.
Parvalbumin isoforms were be discussed. The result supports that Parv1 has a highest
effect on the relaxation of the grunting-toadfish’s sonic muscle.

謝辭....................................................................................................................... i
中文摘要............................................................................................................... ii
英文摘要............................................................................................................... iii
目錄....................................................................................................................... v
圖目錄................................................................................................................... vi
表目錄................................................................................................................... viii
前言....................................................................................................................... 1
實驗材料與方法................................................................................................... 7
實驗結果............................................................................................................... 18
討論....................................................................................................................... 22
參考文獻............................................................................................................... 28
圖........................................................................................................................... 33
表........................................................................................................................... 46
個人履歷............................................................................................................... 48

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