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博碩士論文 etd-0706100-141923 詳細資訊
Title page for etd-0706100-141923
論文名稱
Title
重組生長激素對黑鯛利用飼料蛋白質能力及免疫力之影響
Effects of recombinant growth hormone on dietary protein assimilation and immunity in the black porgy (Acanthopagrus schlegeli)
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
69
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2000-06-12
繳交日期
Date of Submission
2000-07-06
關鍵字
Keywords
蛋白質利用能力、重組生長激素、免疫力、甲硫胺酸、黑鯛
black porgy, methionine, recombinant growth hormone, protein assimilation, immunity
統計
Statistics
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The thesis/dissertation has been browsed 5672 times, has been downloaded 4298 times.
中文摘要
本實驗使用含黑鯛生長激素基因質體(pET-23a-bpGH)的大腸桿菌(BL21),經誘發表達生成黑鯛生長激素,在pH 11.3並有Cysteine催化的條件下,重新復性,再利用離子交換層析及膠體過濾層析純化出單體型態分子量為22 kDa的黑鯛生長激素。研究中進一步使用這些生長激素探討,黑鯛餵飼蛋白質品質不同的飼料時,生長激素刺激對於魚體成長及必需胺基酸蓄積的影響,由替代性補體活性及血清溶菌酵素濃度分析,觀察生長激素對於黑鯛免疫力的影響。本研究為4*3(飼料*生長激素)複因子設計,以魚粉及大豆蛋白和明膠蛋白,依不同比例配製成四種具有不同蛋白質品質的飼料,其比例分別為100 / 0(魚粉 / 大豆蛋白 + 明膠蛋白)、75 / 25、50 / 50、25 / 75。生長激素處理組為不注射、每隔三天注射一次及每隔十二天注射一次,注射於肌肉的生長激素施打劑量為0.05 µg ·g-1。試驗期間共為七十二天。實驗結果顯示:生長激素對黑鯛的增重、飼料效率、蛋白質效率及肌肉中甲硫胺酸濃度均有顯著促進作用,餵食低蛋白質品質飼料的黑鯛受生長激素刺激後,成長表現與餵食高蛋白質品質飼料者並無不同,顯示生長激素可提高黑鯛對於食物中的劣質蛋白質的利用率,促進第一限制胺基酸甲硫胺酸之吸收及蓄積。此外生長激素可活化替代性補體活性並增加血清溶菌酵素濃度,具有增強魚類免疫力之效應。
Abstract
The present study used Escherichia coli(BL21)that contained pET-23a-bpGH plasmids, to express black porgy growth hormone (bpGH). The bpGH was refolded at pH 11.3 in the presence of catalytic amounts of cysteine and purified by ion exchanger chromatography and gel filtration chromatography. The purified bpGH is a monomer and has a molecular weight of 22 kDa. Using the bpGH, the effects of the growth hormone on growth, essential amino acid deposition and nonspecific immunity in black porgy were studied. The experiment was a 4*3 (diet*GH) factorial design. Four experimental diets were formulated in that fish meal was replaced by the mixture of soya protein and gelatin so that fish meal / soya mixture = 100 / 0, 75 / 25, 50 / 50 and 25 / 75, respectively. GH treatments included non-injection, once per 3 days and once per 12 days. GH was injected intramuscularly at a dosage of 0.05 µg / g wet body weight. The growth trial lasted for 72 days. The results showed that GH administration significantly enhanced weight gain, feed efficiency, protein efficiency ratio and muscle methionine concentration of the fish. GH injection improved the growth performance of the fish fed low protein quality diets to a level equals to the groups fed high protein quality diets. These results indicate that GH injections enhanced the perferential absorption and deposition of the first limiting amino acid methionine from the diets. In addition, GH administration enhanced alternative complement activity and increased serum lysozyme concentration, implicating the enhancement of the immunity.
目次 Table of Contents
目錄
章次 頁數

目錄 5
表目錄 6
圖目錄 7
緒論 8
材料與方法 14
結果 35
討論 54
參考文獻 62
參考文獻 References
參考文獻

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