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博碩士論文 etd-0710101-185230 詳細資訊
Title page for etd-0710101-185230
論文名稱
Title
果蠅DmSmt3蛋白與Cactus蛋白交互作用
Interaction Between DmSmt3 and Cactus of Drosophila malenogaster
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
74
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2001-07-02
繳交日期
Date of Submission
2001-07-10
關鍵字
Keywords
果蠅DmSmt3、Cactus
DmSmt3, Cactus
統計
Statistics
本論文已被瀏覽 5699 次,被下載 3125
The thesis/dissertation has been browsed 5699 times, has been downloaded 3125 times.
中文摘要
中文摘要
果蠅Smt3、Cactus、Dorsal分別和脊椎動物SUMO-1、IкBα、NF-кB為同源蛋白,Cactus/IкBα是Rel家族轉錄因子Dorsal/NF-кB的抑制蛋白。研究報告指出,SUMO-1和IкBα結合後可以穩定IкBα,進而抑制NF-кB進入細胞核活化基因表現,而果蠅Smt3則和Dorsal鍵結,使Dorsal可以進入細胞核活化轉錄工作。Smt3/SUMO-1對於Cactus/IкBα和Dorsal/NF-кB的調控在果蠅和脊椎動物中有所不同。
本論文想要探討的是dmSmt3是否會和Cactus鍵結?我們建構了dmSmt3、Cactus、dmubc9等融合蛋白,另外也將Cactus平均分成三段。利用兔子紅血球萃取液和果蠅幼蟲萃取液,進行dmSmt3體外共價鍵結實驗,實驗結果顯示,dmSmt3在體外不會與Cactus共價鍵結,因此這些蛋白質雖然為同源蛋白,但在不同物種中蛋白質交互作用不盡相同。
另外,我們發現cactus與dmSmt3在果蠅幼蟲萃取液進行體外共價鍵結時,cactus有水解的情形產生,此外,單一cac-2融合蛋白可與兔子紅血球萃取液中和果蠅幼蟲萃取液內的許多大分子量蛋白質鍵結,但當cac-2與dmSmt3在果蠅幼蟲萃取液中進行體外共價鍵結反應時,結果只剩下cac-2融合蛋白,因而,dmSmt3可能引發蛋白質水解作用將與cac-2鍵結的蛋白質水解,也可能cac-2鍵結需要的輔助蛋白被dmSmt3水解或是與dmSmt3鍵結,而使得無法與cac-2鍵結。
Abstract
Abstract
DmSmt3, Cactus and Dorsal of Drosophila are homologs of vertebrate SUMO-1, IкBα and NF-кB. Cactus/IкBα is an inhibitor of Dorsal/ NF-кB, the rel family of transcription factor. Conjugation of SUMO-1 to IкBα is thought to stabilize IкBα. The stabilized IкBα inhibits NF-кB to enter the nucleus so that inhibits NF-кB gene expression activation. It was reported that DmSmt3 can bind to Dorsal and allow dorsal to enter the nucleus and activate transcription. It implies that the mechanism of Drosophila is different from vertebrate.
We wonder if DmSmt3 can conjugate to Cactus just like in the vertebrate system. DmSmt3, Cactus and Dmubc9 are cloned and expressed. A series of three deletion of Cactus are constructed (cac-1,cac-2,cac-3). We use reticulocyte lysate and Drosophila larvae extract to perform the in vitro covalent bonding experiment. We find that DmSmt3 does not bind to Cactus. It indicates that though cactus is homologus to IкBα , the interaction may be different among different species.
Otherwise, we find that Cactus degrades in the DmSmt3 covalent bonding experiment in the Drosophila larvae extract. Another finding is that cac-2 can bind to lots of proteins of the reticulocyte lysate and Drosophila larvae extract. But after adding of DmSmt3 in the Drosophila larvae extract, the cac-2 binding proteins are disappeared. We think that DmSmt3 can induce protein degradation mechanism of cac-2 binding proteins, or DmSmt3 degrades the auxiliary proteins which aid cac-2 binding to its target proteins, or DmSmt3 bind to the auxiliary proteins so that auxiliary proteins can not bind to cac-2.
目次 Table of Contents
目錄
壹.中文摘要∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙1
貳.英文摘要∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙2
參.緒論∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙4
一.果蠅Smt3蛋白質介紹∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙4
I.果蠅Smt3的基因結構和分佈∙∙∙∙∙∙∙∙∙∙∙4
II.果蠅Smt3的功能∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙5
二.果蠅Cactus蛋白質介紹∙∙∙∙∙∙∙∙∙∙∙∙∙∙5
I.Cactus的基因結構和分佈∙∙∙∙∙∙∙∙∙∙∙∙6
II.Cactus在胚胎發育所扮演的角色(附錄一) ∙∙∙∙∙∙7
III.Cactus蛋白質結構(附錄二) ∙∙∙∙∙∙∙∙∙∙∙8
IV.Cactus其他功能∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙9
三.哺乳動物中與dmSmt3和Cactus同源蛋白質的研究∙∙∙∙10
I.SUMO-1/dmSmt3對IкBα/Cactus調控的異同∙∙∙∙∙10
II.SUMO-1與其受質蛋白質鍵結區的研究∙∙∙∙∙∙10
四.Smt3和相關蛋白ubiquitin的作用機制∙∙∙∙∙∙∙∙∙∙11
I.ubiquiitn的作用方式(附錄三) ∙∙∙∙∙∙∙∙∙∙∙11
II.Smt3/SUMO-1作用方式(附錄四) ∙∙∙∙∙∙∙∙∙12
五.果蠅Smt3和Cactus可能的關係∙∙∙∙∙∙∙∙∙∙∙∙13
肆.材料∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙14
伍.實驗方法∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙17
一. 從成蠅抽取RNA∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙17
二.寡核甘酸序列的製作∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙17
三.反轉錄聚合脢連鎖反應∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙18
四.質體的建構(TA cloning) ∙∙∙∙∙∙∙∙∙∙∙∙∙∙19
五.質體DNA的少量製備∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙20
六.DNA序列分析∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙21
七.各cDNA轉殖到pQE30、pGEX-4T-3∙∙∙∙∙∙∙∙∙22
八.蛋白質的表現∙∙∙∙∙∙∙∙∙∙∙∙∙∙23
九.SDS PAGE∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙24
十.蛋白質的純化∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙24
I.6-His-融合蛋白的純化∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙25
II.GST-融合蛋白的純化∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙26
III.蛋白質的濃縮和保存∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙27
十一.蛋白質定量∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙27
十二.試管內dmSmt3共價鍵結實驗∙∙∙∙∙∙∙∙∙∙∙∙27
十三.西方漬點法∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙28
十四.果蠅幼蟲蛋白質的萃取∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙29
十五.乳酸脫氫脢活性分析∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙30
陸.實驗結果∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙31
一.各蛋白質的質體建構∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙31
二. 各融合蛋白質的表現及純化∙∙∙∙∙∙∙∙∙∙∙∙31
I.GST-融合蛋白部分∙∙∙∙∙∙∙∙∙∙∙∙∙∙31
II.6-His-融合蛋白部分∙∙∙∙∙∙∙∙∙∙∙∙∙∙32
III.純化後品質∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙32
三.dmSmt3試管內共價鍵結實驗∙∙∙∙∙∙∙∙∙∙∙32
I.以RET進行共價鍵結實驗∙∙∙∙∙∙∙∙∙∙∙∙32
II.以果蠅幼蟲萃取液進行共價鍵結實驗∙∙∙∙∙∙∙34
柒.討論∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙36
一.各融合蛋白的純化∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙36
二.以RET進行體外dmSmt3共價鍵結實驗∙∙∙∙∙∙∙∙36
三. 以果蠅幼蟲萃取液進行體外dmSmt3共價鍵結實驗∙∙∙∙39
四. 總結∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙41
捌.圖表∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙43
玖.附錄∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙61
拾.參考文獻附錄∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙68
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